ecor v digested pbluescript sk plasmid  (Millipore)


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    Structured Review

    Millipore ecor v digested pbluescript sk plasmid
    Ecor V Digested Pbluescript Sk Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v digested pbluescript sk plasmid/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v digested pbluescript sk plasmid - by Bioz Stars, 2020-08
    85/100 stars

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    Incubation:

    Article Title: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous
    Article Snippet: .. The cDNAs were purified on Sephadex G-50 columns (GE Healthcare), mixed with EcoR V-digested pBluescript SK plasmid and 2 U of T4-DNA ligase, incubated at 15°C for 16 h, and dialyzed through a 0.025-μm pore nitrocellulose disk (Millipore). .. The ligation mixture was used to transform E. coli DH5α cells using electroporation, and the white colonies that developed on the LB-amp-XGal plates were analyzed by colony-PCR with the vector primers M13F and M13R.

    Plasmid Preparation:

    Article Title: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous
    Article Snippet: .. The cDNAs were purified on Sephadex G-50 columns (GE Healthcare), mixed with EcoR V-digested pBluescript SK plasmid and 2 U of T4-DNA ligase, incubated at 15°C for 16 h, and dialyzed through a 0.025-μm pore nitrocellulose disk (Millipore). .. The ligation mixture was used to transform E. coli DH5α cells using electroporation, and the white colonies that developed on the LB-amp-XGal plates were analyzed by colony-PCR with the vector primers M13F and M13R.

    Purification:

    Article Title: Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous
    Article Snippet: .. The cDNAs were purified on Sephadex G-50 columns (GE Healthcare), mixed with EcoR V-digested pBluescript SK plasmid and 2 U of T4-DNA ligase, incubated at 15°C for 16 h, and dialyzed through a 0.025-μm pore nitrocellulose disk (Millipore). .. The ligation mixture was used to transform E. coli DH5α cells using electroporation, and the white colonies that developed on the LB-amp-XGal plates were analyzed by colony-PCR with the vector primers M13F and M13R.

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  • 85
    Millipore ecor v digested pbluescript sk plasmid
    Ecor V Digested Pbluescript Sk Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v digested pbluescript sk plasmid/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v digested pbluescript sk plasmid - by Bioz Stars, 2020-08
    85/100 stars
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