ecor i hf  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    EcoRI HF
    Description:
    EcoRI HF 50 000 units
    Catalog Number:
    r3101l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs ecor i hf
    EcoRI HF
    EcoRI HF 50 000 units
    https://www.bioz.com/result/ecor i hf/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecor i hf - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Isolation:

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To set up the linearization reactions, 20 units of EcoRI-HF® (New England Biolabs®) was mixed with 500 ng of isolated DNA (40 units/µg DNA) in CutSmart® buffer (New England Biolabs®), pH 7.9, in a total volume of 50 µL for 30 min at 37 °C. .. The CutSmart® buffer (New England Biolabs®) contains 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 µg/mL BSA.

    Purification:

    Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
    Article Snippet: .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and PstI-HF (NEB) for 20 min at 37°C followed by purification with PCR Clean-up kit (Macherey Nagel). .. Ligation was mediated by T7 DNA ligase (NEB) to construct mUAV, Level 1 and Level 2 Acceptor Vectors.

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Polymerase Chain Reaction:

    Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly
    Article Snippet: .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and PstI-HF (NEB) for 20 min at 37°C followed by purification with PCR Clean-up kit (Macherey Nagel). .. Ligation was mediated by T7 DNA ligase (NEB) to construct mUAV, Level 1 and Level 2 Acceptor Vectors.

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

    Article Title: Efficient Dual-Negative Selection for Bacterial Genome Editing
    Article Snippet: .. Vectors were digested using EcoRI-HF and BamHI-HF (New England BioLabs) for 1h at 37°C, or PCR-amplified, and purified on agarose gel. ..

    Transformation Assay:

    Article Title: Genetic transformation of Spizellomyces punctatus, a resource for studying chytrid biology and evolutionary cell biology
    Article Snippet: .. 2.5 µg of genomic DNA of wild type (WT) and the four transformed strains of Spizellomyces (EM20C-1,2,3,4) was digested in a final volume of 50μL with 100U of EcoRI-HF (NEB R3101S) or HindIII-HF (NEB R3104S) for 24 hr at 37°C. .. After assessing the quality of the digestion by gel electrophoresis, the reaction was heat inactivated and 48µL of the digested DNA was incubated with 1µL of T4 ligase (400u/µL) for 48 hr at 4°C.

    Plasmid Preparation:

    Article Title: Depurination of colibactin-derived interstrand cross-links
    Article Snippet: .. To prepare the linearized DNA, the 2686 bp pUC19 vector (New England Biolabs®) was linearized with 20 units/µg EcoRI-HF® (New England Biolabs®) and the linearized DNA was purified using a PCR clean-up kit (New England Biolabs®), eluted into 10 mM Tris (pH 8.0), and quantified using a nanodrop. .. For each reaction with E.coli , 800 ng of linearized pUC19 DNA was added to 200 µL (6.2 µM base pairs) of M9-CA medium inoculated with 1.2 × 107 bacteria pre-grown to exponential phase in the M9-CA medium.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs ecor i hf
    Ecor I Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i hf/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecor i hf - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    New England Biolabs hf ecor i high fidelity
    Hf Ecor I High Fidelity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hf ecor i high fidelity/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hf ecor i high fidelity - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results