easypack roche protease inhibitor cocktail  (Roche)

 
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    Structured Review

    Roche easypack roche protease inhibitor cocktail
    Easypack Roche Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easypack roche protease inhibitor cocktail/product/Roche
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    easypack roche protease inhibitor cocktail - by Bioz Stars, 2020-04
    89/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Protein expression and purification Full-length yhbE and yhbF were amplified using the following primers: 4309/4305 or 4310/4305 for his 6- yhbE/yhbE and 4306/4308 or 4307/4308 for his 6- yhbF /yhbF , and were each cloned into pET16b (Novagen) or pET24d (Novagen), after digestion with Nco I and Bam HI. .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Amplification:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Protein expression and purification Full-length yhbE and yhbF were amplified using the following primers: 4309/4305 or 4310/4305 for his 6- yhbE/yhbE and 4306/4308 or 4307/4308 for his 6- yhbF /yhbF , and were each cloned into pET16b (Novagen) or pET24d (Novagen), after digestion with Nco I and Bam HI. .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Protease Inhibitor:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics). .. The lysate was then centrifuged for 40 min at 16,000 rpm using JA-25.50 rotor (Beckman Coulter).

    Size-exclusion Chromatography:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Purification was done using Ni-ion affinity and size exclusion chromatography. .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Construct:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Each construct was expressed individually or was coexpressed with the second protein in Escherichia coli BL21 (DE3). .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Purification:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Paragraph title: Protein expression and purification ... The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Expressing:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: Paragraph title: Protein expression and purification ... The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics).

    Lysis:

    Article Title: Bacillus subtilis Bactofilins Are Essential for Flagellar Hook- and Filament Assembly and Dynamically Localize into Structures of Less than 100 nm Diameter underneath the Cell Membrane
    Article Snippet: .. The pellet was resuspended in 10 ml lysis buffer per gram of cells containing 20 mM Na-HEPES pH 7.5, 250 mM NaCl, 10 mM MgCl2 , 10 mM KCl, and 1 tablet of Mini EDTA-free, EASYpack (Roche) protease inhibitor cocktail and pressed through M1-10L Microfluidizer (Microfluidics). .. The lysate was then centrifuged for 40 min at 16,000 rpm using JA-25.50 rotor (Beckman Coulter).

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  • 99
    Roche protease inhibitor cocktail
    Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 8944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
    Average 99 stars, based on 8944 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Roche phosphatase inhibitor cocktail
    Phosphatase Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphatase inhibitor cocktail/product/Roche
    Average 99 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    phosphatase inhibitor cocktail - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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