easy spray ion source  (Thermo Fisher)


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    Name:
    EASY Spray Sources
    Description:
    Nanoflow HPLC separations and nano electrospray ionization can maximize LC MS performance but they rely on numerous critical and error prone connections to achieve optimum results Thermo Scientific EASY Spray Source uses integrated temperature controlled column emitter units that require only a single easy connection The result is state of the art performance narrow peaks maximum separation efficiency and outstanding MS sensitivity for both expert and nonexpert users
    Catalog Number:
    es081
    Price:
    None
    Applications:
    Industrial & Applied Science|Industrial Mass Spectrometry
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher easy spray ion source
    Nanoflow HPLC separations and nano electrospray ionization can maximize LC MS performance but they rely on numerous critical and error prone connections to achieve optimum results Thermo Scientific EASY Spray Source uses integrated temperature controlled column emitter units that require only a single easy connection The result is state of the art performance narrow peaks maximum separation efficiency and outstanding MS sensitivity for both expert and nonexpert users
    https://www.bioz.com/result/easy spray ion source/product/Thermo Fisher
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    easy spray ion source - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Capture of micrococcin biosynthetic intermediates reveals C-terminal processing as an obligatory step for in vivo maturation
    Article Snippet: .. A Thermo Scientific EASY-Spray source and column (PepMap, C18, 3 µm, 100A, 75 µm × 15 µm) was used with a Waters Nano Acquity HPLC system and a Thermo Scientific LTQ-Orbitrap XL mass spectrometer for LCMS analysis. .. Zip-Tipped samples were diluted as appropriate in 0.1% formic acid in water and injected onto the column, which was maintained at 45 °C.

    Flow Cytometry:

    Article Title: Developing a Multiplexed Quantitative Cross-linking Mass Spectrometry Platform for Comparative Structural Analysis of Protein Complexes
    Article Snippet: .. Mixed peptide samples were analyzed utilizing a Thermo Scientific™ EASY-nLC™ 1000 UPLC system coupled on-line to a Thermo Scientific™ Orbitrap Fusion Lumos™ MS. A Thermo Scientific™ EASY-Spray™ source with a 25 cm × 75 μm PepMap EASY-Spray Column was used to separate peptides over a 55 min acetonitrile gradient of 6% to 35% at a flow rate of 300 nL/min. .. Each mixed peptide sample was analyzed using three individual acquisition methods: 1) a targeted ID-MS3 acquisition optimized for DSSO cross-linked peptide identification, 2) a MultiNotch MS3 acquisition featuring synchronous precursor selection (SPS) , and 3) a combined ID-MS3 targeted acquisition with additional SPS-MS3 for all precursor ions selected for ID-MS3 .

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Capture of micrococcin biosynthetic intermediates reveals C-terminal processing as an obligatory step for in vivo maturation
    Article Snippet: .. A Thermo Scientific EASY-Spray source and column (PepMap, C18, 3 µm, 100A, 75 µm × 15 µm) was used with a Waters Nano Acquity HPLC system and a Thermo Scientific LTQ-Orbitrap XL mass spectrometer for LCMS analysis. .. Zip-Tipped samples were diluted as appropriate in 0.1% formic acid in water and injected onto the column, which was maintained at 45 °C.

    Generated:

    Article Title: Identification of Arenin, a Novel Kunitz-Like Polypeptide from the Skin Secretions of Dryophytes arenicolor
    Article Snippet: .. Eluents A (0.1% FA) and B (90% ACN, 0.1% FA) were used to establish the following 60-min gradient: 7–18% B for 30 min, 18–32% B for 15 min, 32–50% B for 4 min, 50–90% B for 1 min, 90–5% B for 0.1 min, 5% B for 7.9 min, 5–7% B for 2 min. Spray was generated using a Thermo Scientific EASY-Spray source (Bremen, Germany) at 1.75 kV. .. QExactive mass spectrometer was set to positive mode for data acquisition with Xcalibur 3.0.63 software (Thermo Fisher Scientific Inc., Bremen, Germany) software alternating between full Fourier transform-mass spectrometry (FT-MS) (350−1600 m / z , resolution 75,000, with 1 μscan per spectrum) and FT-MS/MS (resolution 35000, with 1 μscan per spectrum).

    Quantitation Assay:

    Article Title: Protein lysine de-2-hydroxyisobutyrylation by CobB in prokaryotes
    Article Snippet: .. PRM analysis Quantitation using PRM was performed on an Orbitrap Q Exactive Plus mass spectrometer with an EASY-Spray source coupled to a Nano-LC system (EASY-nLC 1000, Thermo Fisher Scientific, Waltham, MA). ..

    Mass Spectrometry:

    Article Title: Protein lysine de-2-hydroxyisobutyrylation by CobB in prokaryotes
    Article Snippet: .. PRM analysis Quantitation using PRM was performed on an Orbitrap Q Exactive Plus mass spectrometer with an EASY-Spray source coupled to a Nano-LC system (EASY-nLC 1000, Thermo Fisher Scientific, Waltham, MA). ..

    Article Title: The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae
    Article Snippet: .. The ressolubilized peptides were analyzed by liquid chromatography (LC) coupled to an Orbitrap Q-Exactive mass spectrometer (Thermo Scientific) using a nano spray ionization source (Easy-Spray, Thermo Scientific). .. Reverse phase peptide separation was performed with an Ultimate 3000 system (Thermo Scientific) fitted with a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm × 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL min-1 .

    Article Title: Developing a Multiplexed Quantitative Cross-linking Mass Spectrometry Platform for Comparative Structural Analysis of Protein Complexes
    Article Snippet: .. Mixed peptide samples were analyzed utilizing a Thermo Scientific™ EASY-nLC™ 1000 UPLC system coupled on-line to a Thermo Scientific™ Orbitrap Fusion Lumos™ MS. A Thermo Scientific™ EASY-Spray™ source with a 25 cm × 75 μm PepMap EASY-Spray Column was used to separate peptides over a 55 min acetonitrile gradient of 6% to 35% at a flow rate of 300 nL/min. .. Each mixed peptide sample was analyzed using three individual acquisition methods: 1) a targeted ID-MS3 acquisition optimized for DSSO cross-linked peptide identification, 2) a MultiNotch MS3 acquisition featuring synchronous precursor selection (SPS) , and 3) a combined ID-MS3 targeted acquisition with additional SPS-MS3 for all precursor ions selected for ID-MS3 .

    Article Title: Capture of micrococcin biosynthetic intermediates reveals C-terminal processing as an obligatory step for in vivo maturation
    Article Snippet: .. A Thermo Scientific EASY-Spray source and column (PepMap, C18, 3 µm, 100A, 75 µm × 15 µm) was used with a Waters Nano Acquity HPLC system and a Thermo Scientific LTQ-Orbitrap XL mass spectrometer for LCMS analysis. .. Zip-Tipped samples were diluted as appropriate in 0.1% formic acid in water and injected onto the column, which was maintained at 45 °C.

    Article Title: A Proteomic Approach to Identify Alterations in the Small Ubiquitin-like Modifier (SUMO) Network during Controlled Mechanical Ventilation in Rat Diaphragm Muscle *
    Article Snippet: .. The peptides from each gel section were analyzed using a Q Exactive HF Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) equipped with an EASY-Spray ion source (Thermo Fisher Scientific). .. The peptides were separated by reversed phase LC using a Dionex UltiMate 3000 RSLC nanosystem (Thermo Fisher Scientific).

    Article Title: Cytoplasmic glycoengineering of Apx toxin fragments in the development of Actinobacillus pleuropneumoniae glycoconjugate vaccines
    Article Snippet: .. The eluted peptides from C18 column LC eluant were sprayed into the mass spectrometer by means of an Easy-Spray source (Thermo Fisher Scientific Inc.). ..

    Liquid Chromatography:

    Article Title: The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae
    Article Snippet: .. The ressolubilized peptides were analyzed by liquid chromatography (LC) coupled to an Orbitrap Q-Exactive mass spectrometer (Thermo Scientific) using a nano spray ionization source (Easy-Spray, Thermo Scientific). .. Reverse phase peptide separation was performed with an Ultimate 3000 system (Thermo Scientific) fitted with a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm × 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN, 0.1% FA at 10 μL min-1 .

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  • Bioz Stars
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  • 99
    Thermo Fisher easy spray ion source
    Easy Spray Ion Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easy spray ion source/product/Thermo Fisher
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    easy spray ion source - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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    Thermo Fisher easy spray nanoelectrospray ion source
    Easy Spray Nanoelectrospray Ion Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easy spray nanoelectrospray ion source/product/Thermo Fisher
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    easy spray nanoelectrospray ion source - by Bioz Stars, 2020-07
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