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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors <t>E64d</t> and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.
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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors <t>E64d</t> and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.
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Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors E64d and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.

Journal: bioRxiv

Article Title: Functional Landscape of Motifs within the Sarbecovirus Spike Cytoplasmic Tail

doi: 10.64898/2026.05.06.723231

Figure Lengend Snippet: Neutralization curves of VSV pseudoviruses carrying FL, Δ13, and Δ17 spike to the indicated monoclonal neutralizing antibodies ( a ), serum samples collected after SARS-CoV-2 pandemic ( b ), hACE2 ( c ), and the protease inhibitors E64d and camostat ( d ). Antibody and serum neutralization assays, as well as hACE2 inhibition assays, were performed in Vero E6 cells, whereas protease inhibitor assays were conducted in Vero E6-ACE2-TMPRSS2 cells. Numbers in each panel denote IC 50 or ID 50 of pseudoviruses carrying FL (black), Δ13 (red), and Δ17 (blue) spikes.

Article Snippet: To test the effect of host protease inhibitors on pseudovirus entry, Vero E6-ACE2-TMPRSS2 cells (4×104 cells/well) were pre-incubated for 2 hours with serially diluted cathepsin L inhibitor E64d (MedChemExpress, CAS# 88321-09-9) or TMPRSS2 inhibitor camostat mesylate (MedChemExpress, CAS# 59721-29-8).

Techniques: Neutralization, Inhibition, Protease Inhibitor