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one-step tunel in situ apoptosis kit (green - by Bioz Stars, 2026-07
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Therapeutic effects of M2-EVs@DNase I on sepsis-induced liver and kidney injury. (A-D) Serum levels of ALT, AST, UREA, and CREZ in each group. (E, F) Quantification of M1 (F4/80 + iNOS + ) and M2 (F4/80 + CD206 + ) macrophages, respectively. (G) Quantification of <t>TUNEL-positive</t> cells. (H) Quantitative analysis of mean fluorescence intensity of KIM-1. (I) Representative CLSM images of immunofluorescence staining for F4/80 (pink), iNOS (green), and DAPI (blue) of kidney tissues from each group. (J) Representative CLSM images of immunofluorescence staining for F4/80 (pink), CD206 (green), and DAPI (blue) of kidney tissues from each group. (K) Representative TUNEL staining images (green) with DAPI nuclear counterstaining (blue). (L) Representative CLSM images of immunofluorescence staining for KIM-1 (green) and DAPI (blue) in the renal tissues. Scale bar = 50 μm. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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Therapeutic effects of M2-EVs@DNase I on sepsis-induced liver and kidney injury. (A-D) Serum levels of ALT, AST, UREA, and CREZ in each group. (E, F) Quantification of M1 (F4/80 + iNOS + ) and M2 (F4/80 + CD206 + ) macrophages, respectively. (G) Quantification of <t>TUNEL-positive</t> cells. (H) Quantitative analysis of mean fluorescence intensity of KIM-1. (I) Representative CLSM images of immunofluorescence staining for F4/80 (pink), iNOS (green), and DAPI (blue) of kidney tissues from each group. (J) Representative CLSM images of immunofluorescence staining for F4/80 (pink), CD206 (green), and DAPI (blue) of kidney tissues from each group. (K) Representative TUNEL staining images (green) with DAPI nuclear counterstaining (blue). (L) Representative CLSM images of immunofluorescence staining for KIM-1 (green) and DAPI (blue) in the renal tissues. Scale bar = 50 μm. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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cat e ck a320 - by Bioz Stars, 2026-07
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Therapeutic effects of M2-EVs@DNase I on sepsis-induced liver and kidney injury. (A-D) Serum levels of ALT, AST, UREA, and CREZ in each group. (E, F) Quantification of M1 (F4/80 + iNOS + ) and M2 (F4/80 + CD206 + ) macrophages, respectively. (G) Quantification of <t>TUNEL-positive</t> cells. (H) Quantitative analysis of mean fluorescence intensity of KIM-1. (I) Representative CLSM images of immunofluorescence staining for F4/80 (pink), iNOS (green), and DAPI (blue) of kidney tissues from each group. (J) Representative CLSM images of immunofluorescence staining for F4/80 (pink), CD206 (green), and DAPI (blue) of kidney tissues from each group. (K) Representative TUNEL staining images (green) with DAPI nuclear counterstaining (blue). (L) Representative CLSM images of immunofluorescence staining for KIM-1 (green) and DAPI (blue) in the renal tissues. Scale bar = 50 μm. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Deoxynucleotidyl Transferase Dutp Nick End Labeling Tunel Staining, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Therapeutic effects of M2-EVs@DNase I on sepsis-induced liver and kidney injury. (A-D) Serum levels of ALT, AST, UREA, and CREZ in each group. (E, F) Quantification of M1 (F4/80 + iNOS + ) and M2 (F4/80 + CD206 + ) macrophages, respectively. (G) Quantification of TUNEL-positive cells. (H) Quantitative analysis of mean fluorescence intensity of KIM-1. (I) Representative CLSM images of immunofluorescence staining for F4/80 (pink), iNOS (green), and DAPI (blue) of kidney tissues from each group. (J) Representative CLSM images of immunofluorescence staining for F4/80 (pink), CD206 (green), and DAPI (blue) of kidney tissues from each group. (K) Representative TUNEL staining images (green) with DAPI nuclear counterstaining (blue). (L) Representative CLSM images of immunofluorescence staining for KIM-1 (green) and DAPI (blue) in the renal tissues. Scale bar = 50 μm. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: International Journal of Pharmaceutics: X

Article Title: Engineered M2 macrophage-derived vesicles deliver DNase I for cfDNA clearance and multi-organ protection in sepsis

doi: 10.1016/j.ijpx.2026.100528

Figure Lengend Snippet: Therapeutic effects of M2-EVs@DNase I on sepsis-induced liver and kidney injury. (A-D) Serum levels of ALT, AST, UREA, and CREZ in each group. (E, F) Quantification of M1 (F4/80 + iNOS + ) and M2 (F4/80 + CD206 + ) macrophages, respectively. (G) Quantification of TUNEL-positive cells. (H) Quantitative analysis of mean fluorescence intensity of KIM-1. (I) Representative CLSM images of immunofluorescence staining for F4/80 (pink), iNOS (green), and DAPI (blue) of kidney tissues from each group. (J) Representative CLSM images of immunofluorescence staining for F4/80 (pink), CD206 (green), and DAPI (blue) of kidney tissues from each group. (K) Representative TUNEL staining images (green) with DAPI nuclear counterstaining (blue). (L) Representative CLSM images of immunofluorescence staining for KIM-1 (green) and DAPI (blue) in the renal tissues. Scale bar = 50 μm. n = 3. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The One-Step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488) was bought from Elabscience® Biotechnology Co., Ltd. (Wuhan, China).

Techniques: TUNEL Assay, Fluorescence, Immunofluorescence, Staining