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GE Healthcare e xo sap
E Xo Sap, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 7498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e xo sap/product/GE Healthcare
Average 95 stars, based on 7498 article reviews
Price from $9.99 to $1999.99
e xo sap - by Bioz Stars, 2020-12
95/100 stars

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DNA Extraction:

Article Title: Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students
Article Snippet: .. Bacterial DNA extraction was performed by using the Illustra tissue and cells prep genomic mini spin kit (GE Healthcare, Little Chalfont, UK), according to manufacturer´s instructions, following an adapted protocol described by Pereira et al. [ ]. ..

Amplification:

Article Title: Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population
Article Snippet: .. After PCR amplification, amplicons were processed with Illustra Exonuclease I and Alkaline Phosphatase (GE Healthcare and Life Science, Little Chalfont, United Kingdom) to digest excess primers. .. The cycle sequencing reaction was then performed with the Big Dye Terminator v3.1 Cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Combining GWAS and RNA-Seq Approaches for Detection of the Causal Mutation for Hereditary Junctional Epidermolysis Bullosa in Sheep
Article Snippet: .. The amplicons were purified using Illustra Exonuclease I and alkaline phosphatase (Illustra ExoProStar 1-Step, GE Healthcare Life Sciences) treatment and were dideoxy-sequenced in both directions using the Big Dye Terminator Cycle Sequencing Kit, v3.1 (Applied Biosystems) using the same primers used for the fragment amplification. .. After sequencing on an ABI Prism 3130 automatic sequencer (Applied Biosystems), the sequence data were analyzed using SeqScape v2.5 (Applied Biosystems).

Article Title: Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
Article Snippet: .. After the RT reaction, primer-dimers and small fragments were removed by 0.5 U Exonuclease (GE Healthcare, E70073Z) treatment for 1 h at 42 °C. cDNA libraries were amplified with KAPA Hifi HotStart ReadyMix (KAPA Biosystems, KK2602) and custom PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT-3’) with the following PCR program: 1) 98 °C 3 min, 25 cycles of 98 °C 20 s, 67 °C 15 s 72 C 6 min, 3) 72 C 5 min. cDNA libraries of 84 cells were multiplexed and short fragments were eliminated by Agencourt Ampure XP beads (Beckman Coulter, A63880, ratio of 0.8:1 beads to library volume). .. The quality of multiplexed cDNA libraries was examined with a 2100 Bioanalyzer (Agilent) according to the manufacturer’s protocol. cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024).

Isolation:

Article Title: Galectin-1-deficient mice are protected against Trypanosoma cruzi infection through altered neutrophil migration and production of reactive oxygen species and nitric oxide
Article Snippet: .. The genomic DNA was isolated using Illustra™ Tissue and cell genomic Prep mini spin kit (GE, USA), according to the manufacturer’s instructions. .. The amount of recovered genomic DNA was quantified with NanoDrop ND 2000 (Thermo Scientific, USA) and stored at −20 °C.

Purification:

Article Title: Combining GWAS and RNA-Seq Approaches for Detection of the Causal Mutation for Hereditary Junctional Epidermolysis Bullosa in Sheep
Article Snippet: .. The amplicons were purified using Illustra Exonuclease I and alkaline phosphatase (Illustra ExoProStar 1-Step, GE Healthcare Life Sciences) treatment and were dideoxy-sequenced in both directions using the Big Dye Terminator Cycle Sequencing Kit, v3.1 (Applied Biosystems) using the same primers used for the fragment amplification. .. After sequencing on an ABI Prism 3130 automatic sequencer (Applied Biosystems), the sequence data were analyzed using SeqScape v2.5 (Applied Biosystems).

Polymerase Chain Reaction:

Article Title: Mutation screening of GRIN2B in schizophrenia and autism spectrum disorder in a Japanese population
Article Snippet: .. After PCR amplification, amplicons were processed with Illustra Exonuclease I and Alkaline Phosphatase (GE Healthcare and Life Science, Little Chalfont, United Kingdom) to digest excess primers. .. The cycle sequencing reaction was then performed with the Big Dye Terminator v3.1 Cycle sequencing kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Profiling microglia from AD donors and non-demented elderly in acute human post-mortem cortical tissue
Article Snippet: .. After the RT reaction, primer-dimers and small fragments were removed by 0.5 U Exonuclease (GE Healthcare, E70073Z) treatment for 1 h at 42 °C. cDNA libraries were amplified with KAPA Hifi HotStart ReadyMix (KAPA Biosystems, KK2602) and custom PCR primer (5’-AAGCAGTGGTATCAACGCAGAGT-3’) with the following PCR program: 1) 98 °C 3 min, 25 cycles of 98 °C 20 s, 67 °C 15 s 72 C 6 min, 3) 72 C 5 min. cDNA libraries of 84 cells were multiplexed and short fragments were eliminated by Agencourt Ampure XP beads (Beckman Coulter, A63880, ratio of 0.8:1 beads to library volume). .. The quality of multiplexed cDNA libraries was examined with a 2100 Bioanalyzer (Agilent) according to the manufacturer’s protocol. cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024).

Sequencing:

Article Title: Combining GWAS and RNA-Seq Approaches for Detection of the Causal Mutation for Hereditary Junctional Epidermolysis Bullosa in Sheep
Article Snippet: .. The amplicons were purified using Illustra Exonuclease I and alkaline phosphatase (Illustra ExoProStar 1-Step, GE Healthcare Life Sciences) treatment and were dideoxy-sequenced in both directions using the Big Dye Terminator Cycle Sequencing Kit, v3.1 (Applied Biosystems) using the same primers used for the fragment amplification. .. After sequencing on an ABI Prism 3130 automatic sequencer (Applied Biosystems), the sequence data were analyzed using SeqScape v2.5 (Applied Biosystems).

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    GE Healthcare e xo sap
    E Xo Sap, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e xo sap/product/GE Healthcare
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e xo sap - by Bioz Stars, 2020-12
    95/100 stars
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