e scherichia coli novablue de3 competent cell  (Millipore)


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    Structured Review

    Millipore e scherichia coli novablue de3 competent cell
    E Scherichia Coli Novablue De3 Competent Cell, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e scherichia coli novablue de3 competent cell/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e scherichia coli novablue de3 competent cell - by Bioz Stars, 2020-03
    84/100 stars

    Related Products / Commonly Used Together

    pet32a-klcbf1

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    Related Articles

    Clone Assay:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: Paragraph title: Cloning of the KlCBF1 Gene and Cbf1 Protein Purification ... The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction.

    Amplification:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: Cloning of the KlCBF1 Gene and Cbf1 Protein Purification The coding region of CBF1 was amplified from the K. lactis KB101 strain genomic DNA and cloned into the pET32 plasmid (named as pET32a-KlCBF1 ) with XhoI (New England Biolabs) and EcoRI (New England Biolabs) sites. .. The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction.

    Protease Inhibitor:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction. .. IPTG was added to the culture to the final concentration of 1 mM, and the induced culture was grown for additional 4 h. Cells were then centrifuged to harvest and resuspended in 25 ml binding buffer (20 mM Tris-HCl, 300 mM NaCl, pH 7.5), with protease inhibitor (protease Inhibitor cocktail set I, Calbiochem) to prevent protein degradation.

    Protein Purification:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: Paragraph title: Cloning of the KlCBF1 Gene and Cbf1 Protein Purification ... The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction.

    Concentration Assay:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction. .. IPTG was added to the culture to the final concentration of 1 mM, and the induced culture was grown for additional 4 h. Cells were then centrifuged to harvest and resuspended in 25 ml binding buffer (20 mM Tris-HCl, 300 mM NaCl, pH 7.5), with protease inhibitor (protease Inhibitor cocktail set I, Calbiochem) to prevent protein degradation.

    Transformation Assay:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: .. The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction. ..

    Binding Assay:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction. .. IPTG was added to the culture to the final concentration of 1 mM, and the induced culture was grown for additional 4 h. Cells were then centrifuged to harvest and resuspended in 25 ml binding buffer (20 mM Tris-HCl, 300 mM NaCl, pH 7.5), with protease inhibitor (protease Inhibitor cocktail set I, Calbiochem) to prevent protein degradation.

    Plasmid Preparation:

    Article Title: Identifying Cis-Regulatory Changes Involved in the Evolution of Aerobic Fermentation in Yeasts
    Article Snippet: Cloning of the KlCBF1 Gene and Cbf1 Protein Purification The coding region of CBF1 was amplified from the K. lactis KB101 strain genomic DNA and cloned into the pET32 plasmid (named as pET32a-KlCBF1 ) with XhoI (New England Biolabs) and EcoRI (New England Biolabs) sites. .. The pET32a-KlCBF1 was then transformed into E scherichia coli NovaBlue (DE3) competent cell (Novagen) for protein induction.