e faecium l50 14  (Millipore)


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    Name:
    Lipopolysaccharides rough strains from Escherichia coli J5
    Description:

    Catalog Number:
    l5014
    Price:
    None
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    Structured Review

    Millipore e faecium l50 14

    https://www.bioz.com/result/e faecium l50 14/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e faecium l50 14 - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma). .. After 24h, culture media was collected for ELISA and cell or tissue lysates were harvested for Western blot.

    Immunohistochemistry:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide ( Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide (Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.

    Expressing:

    Article Title: Cloning and Heterologous Production of Hiracin JM79, a Sec-Dependent Bacteriocin Produced by Enterococcus hirae DCH5, in Lactic Acid Bacteria and Pichia pastoris
    Article Snippet: Transformants of Lactobacillus lactis , L. sakei Lb790, Enterococcus faecalis JH2-2, and E. faecium L50/14-2 were selected with 5 μg/ml of chloramphenicol (Sigma Chemical Co., St. Louis, MO). .. An EasySelect Pichia expression kit, the expression vector pPICZαA, the wild-type P. pastoris X-33 host, and zeocin were obtained from Invitrogen Life Technologies (Madrid, Spain).

    Plasmid Preparation:

    Article Title: Cloning and Heterologous Production of Hiracin JM79, a Sec-Dependent Bacteriocin Produced by Enterococcus hirae DCH5, in Lactic Acid Bacteria and Pichia pastoris
    Article Snippet: Transformants of Lactobacillus lactis , L. sakei Lb790, Enterococcus faecalis JH2-2, and E. faecium L50/14-2 were selected with 5 μg/ml of chloramphenicol (Sigma Chemical Co., St. Louis, MO). .. An EasySelect Pichia expression kit, the expression vector pPICZαA, the wild-type P. pastoris X-33 host, and zeocin were obtained from Invitrogen Life Technologies (Madrid, Spain).

    Polymerase Chain Reaction:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma). .. Total RNA was extracted from the cells or tissue explants using the RNeasy Mini Kit (Qiagen) for PCR.

    Protease Inhibitor:

    Article Title: TAK-242, a small-molecule inhibitor of Toll-like receptor 4 signalling, unveils similarities and differences in lipopolysaccharide- and lipidinduced inflammation and insulin resistance in muscle cells
    Article Snippet: .. LPS from Escherichia coli J5 (L5014), stearate (S4751), cytochalasin B, 2-DG, protease inhibitor cocktail and other chemicals, unless otherwise noted, were from Sigma. .. Insulin (Novolin-R) was obtained from Novo Nordisk.

    CCK-8 Assay:

    Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
    Article Snippet: LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). .. LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA).

    Cell Culture:

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: Stimulation assays Primary RASFs were isolated and cultured as described previously [ ], and cells were used between passages 3 and 7. .. RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA).

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: Primary RASFs were isolated and cultured as described previously [ ], and cells were used between passages 3 and 7. .. RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
    Article Snippet: LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). .. Reagents for quantitative polymerase chain reaction (qPCR) analysis, including reverse transcriptase kit from Promega (Madison, WI, USA) and Thunderbird SYBR qPCR Mix from Toyobo, Co., Ltd., (Osaka, Japan).

    Activation Assay:

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA). .. Activation of PI3K/AKT pathways was blocked using LY294002 (Calbiochem, MCE, New Jersey, USA) and NF-κB inhibitor PDTC (M4005, Abmole Bioscience, Hong Kong, China).

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA). .. Activation of PI3K/AKT pathways was blocked using LY294002 (Calbiochem, MCE, New Jersey, USA) and NF-κB inhibitor PDTC (M4005, Abmole Bioscience, Hong Kong, China).

    other:

    Article Title: Fetal Fibronectin Signaling Induces Matrix Metalloproteases and Cyclooxygenase-2 (COX-2) in Amnion Cells and Preterm Birth in Mice *
    Article Snippet: Phenylmethanesulfonyl fluoride (PMSF; P7626) and lipopolysaccharides (rough strains) from Escherichia coli J5 (L-5014) were purchased from Sigma.

    Activity Assay:

    Article Title: Cloning and Heterologous Production of Hiracin JM79, a Sec-Dependent Bacteriocin Produced by Enterococcus hirae DCH5, in Lactic Acid Bacteria and Pichia pastoris
    Article Snippet: Paragraph title: Bacterial strains, plasmids, growth conditions, and antimicrobial activity assays. ... Transformants of Lactobacillus lactis , L. sakei Lb790, Enterococcus faecalis JH2-2, and E. faecium L50/14-2 were selected with 5 μg/ml of chloramphenicol (Sigma Chemical Co., St. Louis, MO).

    Infection:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: .. Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide ( Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: .. Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide (Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.

    Quantitative RT-PCR:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide ( Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Protein and mRNA were harvested from the cells and tissue explants for Western blot or qRT-PCR to quantify thrombin/prothrombin protein or mRNA production, respectively.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide (Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Protein and mRNA were harvested from the cells and tissue explants for Western blot or qRT-PCR to quantify thrombin/prothrombin protein or mRNA production, respectively.

    Isolation:

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: Stimulation assays Primary RASFs were isolated and cultured as described previously [ ], and cells were used between passages 3 and 7. .. RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA).

    Article Title: E2F2 directly regulates the STAT1 and PI3K/AKT/NF-κB pathways to exacerbate the inflammatory phenotype in rheumatoid arthritis synovial fibroblasts and mouse embryonic fibroblasts
    Article Snippet: Primary RASFs were isolated and cultured as described previously [ ], and cells were used between passages 3 and 7. .. RASFs were plated in 24-well plates (3–5 × 105 cells/well) and stimulated for 12 h with LPS (from Escherichia coli J5; Sigma, St Louis, MO, USA).

    Modification:

    Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
    Article Snippet: Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin, Basement Membrane Matrix and phosphate-buffered saline (PBS) were obtained from Invitrogen™ (Thermo Fisher Scientific, Inc., Waltham, MA, USA). .. LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA).

    Western Blot:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide ( Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Protein and mRNA were harvested from the cells and tissue explants for Western blot or qRT-PCR to quantify thrombin/prothrombin protein or mRNA production, respectively.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma). .. After 24h, culture media was collected for ELISA and cell or tissue lysates were harvested for Western blot.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide (Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Protein and mRNA were harvested from the cells and tissue explants for Western blot or qRT-PCR to quantify thrombin/prothrombin protein or mRNA production, respectively.

    Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
    Article Snippet: .. LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). .. Antibodies against HIF-1α, epidermal growth factor (EGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF2), TLR4, caspase-3, p-ASK1, p-p38 and myeloid differentiation primary response gene 88 (MyD88) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Hybridization:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: All cell types or tissue explants were confirmed to be free of Mycoplasma and Ureaplasma contamination using a chemiluminescent-labelled single-stranded DNA probe hybridization method (MTC-NI kit, Millipore). .. Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma).

    Immunofluorescence:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells were plated in the same culture conditions as amnion cells for 48 h. Purity of primary cells was confirmed using immunofluorescence staining for cytokeratin and vimentin to distinguish amnion/chorion from decidua cells. .. Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma).

    Derivative Assay:

    Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
    Article Snippet: .. LPS derived from Escherichia coli J5 (catalog no., L5014) was obtained from Sigma-Aldrich (St. Louis, MO, USA) and ECL® Plus Western Blotting Detection System was from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). .. Antibodies against HIF-1α, epidermal growth factor (EGF), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF2), TLR4, caspase-3, p-ASK1, p-p38 and myeloid differentiation primary response gene 88 (MyD88) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Staining:

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide ( Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells were plated in the same culture conditions as amnion cells for 48 h. Purity of primary cells was confirmed using immunofluorescence staining for cytokeratin and vimentin to distinguish amnion/chorion from decidua cells. .. Tissue explants (0.5 cm × 0.5 cm) and primary cells (2×106 cells/well in 6-well plates) were treated with and without live U. parvum (1×105 , 1×106 , or 1×107 cfu/ml) or lipopolysaccharide (LPS, 100ng/ml or 1000ng/ml, Escherichia coli J5, L-5014, Cat# L5014, Sigma).

    Article Title: Infection-Induced Thrombin Production: A Potential Novel Mechanism for Preterm Premature Rupture of Membranes (PPROM)
    Article Snippet: Cells or tissue explants were infected with live U. parvum (1 × 105 , 1 × 106 , or 1 × 107 colony forming units (cfu)/ml) or lipopolysaccharide (Escherichia coli J5, L-5014, Sigma, 100 ng/ml or 1000 ng/ml) for 24 hours. .. Tissue explants were fixed for immunohistochemistry staining of thrombin/prothrombin.