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Becton Dickinson e coli xl1 blue
E Coli Xl1 Blue, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli xl1 blue/product/Becton Dickinson
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
e coli xl1 blue - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

Clone Assay:

Article Title: Isoxyl Activation Is Required for Bacteriostatic Activity against Mycobacterium tuberculosis ▿
Article Snippet: .. Escherichia coli XL1-blue, the strain used for cloning experiments, was propagated in Luria-Bertani broth (pH 7.5) (Bactotryptone [10 g/liter], Bacto yeast extract [5 g/liter], NaCl [5 g/liter]) (Becton Dickinson, Sparks, MD). .. M. smegmatis mc2 155 was grown in Middlebrook 7H9 medium (Difco) supplemented with ADC and 0.05% Tween 80, Luria-Bertani broth supplemented with 0.05% Tween 80, or MM63 minimal medium supplemented with 0.025% tyloxapol.

Article Title: Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: Biosynthesis and Impact on the Persistence in mice
Article Snippet: .. E. coli XL1-blue, the strain used for cloning experiments, was propagated in Luria Bertani (LB) broth (pH 7.5) (Becton Dickinson, Sparks, MD). .. M. tuberculosis H37Rv was grown at 37°C in Middlebrook 7H9 broth supplemented with 10% ADC enrichment (Difco) and 0.05% Tween 80, in minimal Sauton's medium as surface pellicles or on agar Middlebrook 7H11 medium supplemented with OADC (Difco).

Article Title: Bordetella pertussis Expresses a Functional Type III Secretion System That Subverts Protective Innate and Adaptive Immune Responses ▿
Article Snippet: .. For routine cloning and conjugation, E. coli XL1-Blue and SM10λpir were grown at 37°C on Luria-Bertani (LB) agar or LB broth (BD Difco) supplemented with the appropriate antibiotics (ampicillin, 150 μg/ml; gentamicin, 10 μg/ml; kanamycin, 25 μg/ml). .. Gentamicin-resistant Δ bscN derivatives of B. pertussis 12743 and B. bronchiseptica RB50, in which a 0.5-kb internal portion of the bscN gene was removed and replaced with a 0.7-kb fragment containing a gentamicin resistance cassette, were constructed as follows.

Selection:

Article Title: Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: Biosynthesis and Impact on the Persistence in mice
Article Snippet: E. coli XL1-blue, the strain used for cloning experiments, was propagated in Luria Bertani (LB) broth (pH 7.5) (Becton Dickinson, Sparks, MD). .. For selection of allelic exchange mutants, 2% sucrose was added to the solid medium.

Conjugation Assay:

Article Title: Bordetella pertussis Expresses a Functional Type III Secretion System That Subverts Protective Innate and Adaptive Immune Responses ▿
Article Snippet: .. For routine cloning and conjugation, E. coli XL1-Blue and SM10λpir were grown at 37°C on Luria-Bertani (LB) agar or LB broth (BD Difco) supplemented with the appropriate antibiotics (ampicillin, 150 μg/ml; gentamicin, 10 μg/ml; kanamycin, 25 μg/ml). .. Gentamicin-resistant Δ bscN derivatives of B. pertussis 12743 and B. bronchiseptica RB50, in which a 0.5-kb internal portion of the bscN gene was removed and replaced with a 0.7-kb fragment containing a gentamicin resistance cassette, were constructed as follows.

Mutagenesis:

Article Title: Isoxyl Activation Is Required for Bacteriostatic Activity against Mycobacterium tuberculosis ▿
Article Snippet: M. tuberculosis H37Rv (ATCC 25618), the mutT1 transposon mutant of M. tuberculosis Mt103 (MT1K) , and M. bovis BCG 1173P2 (Pasteur strain) were grown in Middlebrook 7H9 medium (Difco) supplemented with ADC (0.2% dextrose, 0.5% bovine serum albumin fraction V, 0.085% NaCl, 0.0003% beef catalase) and 0.05% Tween 80 or on solid Middlebrook 7H11 medium (Difco) supplemented with OADC (0.005% oleic acid, 0.2% dextrose, 0.5% bovine serum albumin fraction V, 0.085% NaCl, 0.0003% beef catalase). .. Escherichia coli XL1-blue, the strain used for cloning experiments, was propagated in Luria-Bertani broth (pH 7.5) (Bactotryptone [10 g/liter], Bacto yeast extract [5 g/liter], NaCl [5 g/liter]) (Becton Dickinson, Sparks, MD).

Isolation:

Article Title: Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
Article Snippet: Isolates A1-6, B1-2 and C1-2 were thought to belong to the different clonal groups because the strains were isolated from the same farm at the same time and showed the same genotyping results. .. Escherichia coli XL1-Blue were grown in Luria-Bertani medium (BD, Becton, Dickinson Co.).

Cell Culture:

Article Title: Genomic and Biochemical Characterization of Acinetobacter Podophage Petty Reveals a Novel Lysis Mechanism and Tail-Associated Depolymerase Activity
Article Snippet: .. Recombinant strains of E. coli XL1-Blue and BL21(DE3) were cultured on LB (10 g/liter of Bacto tryptone [BD], 5 g/liter of yeast extract, 10 g/liter of NaCl) with kanamycin (40 μg/ml). ..

Article Title: Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
Article Snippet: The A. pleuropneumoniae isolates were cultured in chocolate II agar (BD, Becton, Dickinson Co., Detroit, MI, U.S.A.) or in heart infusion (HI) medium (BD) supplemented with 0.3% yeast extract (dried yeast extract-S, Nippon Seiyaku, Tokyo, Japan) and 0.005% β-nicotinamide adenine dinucleotide (NAD) (Oriental Yeast, Tokyo, Japan). .. Escherichia coli XL1-Blue were grown in Luria-Bertani medium (BD, Becton, Dickinson Co.).

Concentration Assay:

Article Title: Bordetella pertussis Expresses a Functional Type III Secretion System That Subverts Protective Innate and Adaptive Immune Responses ▿
Article Snippet: For allelic exchange WT B. pertussis 12743 was first rendered streptomycin resistant by subculture in increasing sublethal concentrations of streptomycin (final concentration, 100 μg/ml). .. For routine cloning and conjugation, E. coli XL1-Blue and SM10λpir were grown at 37°C on Luria-Bertani (LB) agar or LB broth (BD Difco) supplemented with the appropriate antibiotics (ampicillin, 150 μg/ml; gentamicin, 10 μg/ml; kanamycin, 25 μg/ml).

other:

Article Title: LppX is a lipoprotein required for the translocation of phthiocerol dimycocerosates to the surface of Mycobacterium tuberculosis
Article Snippet: E. coli XL1-blue and BL21DE3pLysS were maintained in Luria Bertani (LB) broth (pH 7.5) (Becton Dickinson, Sparks, MD).

Infection:

Article Title: Isolation and characterization of atypical Actinobacillus pleuropneumoniae serovar 15 lacking the apxIICA genes in Japan
Article Snippet: Six atypical strains of serovar 15 (A1, A2, A3, A4, A5, A6) isolated from the lung lesions of pneumonic naturally infected, dead pigs from farm A, and five representative field strains (B1, B2, C1, C2, D1) of serovar 15 isolated from farms B, C and D in Japan were used in this study. .. Escherichia coli XL1-Blue were grown in Luria-Bertani medium (BD, Becton, Dickinson Co.).

Recombinant:

Article Title: Genomic and Biochemical Characterization of Acinetobacter Podophage Petty Reveals a Novel Lysis Mechanism and Tail-Associated Depolymerase Activity
Article Snippet: .. Recombinant strains of E. coli XL1-Blue and BL21(DE3) were cultured on LB (10 g/liter of Bacto tryptone [BD], 5 g/liter of yeast extract, 10 g/liter of NaCl) with kanamycin (40 μg/ml). ..

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    Becton Dickinson e coli xl1 blue
    E Coli Xl1 Blue, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue/product/Becton Dickinson
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue - by Bioz Stars, 2020-04
    92/100 stars
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