e coli xl1 blue supercompetent cells  (Millipore)


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    Structured Review

    Millipore e coli xl1 blue supercompetent cells
    E Coli Xl1 Blue Supercompetent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue supercompetent cells/product/Millipore
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue supercompetent cells - by Bioz Stars, 2020-04
    89/100 stars

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    Related Articles

    Amplification:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen). .. Plasmid DNA was extracted (QIAprep Spin Miniprep Kit, Qiagen) and verified by DNA sequencing (DNA Sequencing Unit, University of Dundee).

    In Vitro:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: Protein production and purification of the Pa FabF C164Q mutant In vitro site-directed mutagenesis using the Agilent QuikChange kit was carried out using the Pa FabF gene carrying the pNic28-Bsa4 vector to mutate Cys164 to Gln164. .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Methylation:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The PCR product generated using the forward primer 5′-GCCCTCACCACCGCC CAG ACCACCGGTACCCAC-3′ and the reverse primer 5′-GTGGGTACCGGTGGT CTG GGCGGTGGTGAGGGC-3′ was treated with the DpnI restriction enzyme (Biolabs) to digest the methylated template plasmid. .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Mutagenesis:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: Paragraph title: Protein production and purification of the Pa FabF C164Q mutant   ... The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Purification:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: Paragraph title: Protein production and purification of the Pa FabF C164Q mutant   ... The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Protein Purification:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen). .. Overexpression and protein purification were performed as described for wild-type Pa FabF but using phosphate buffer [50 mM Na2 HPO4 pH 7.8, 300 mM NaCl, 10%(v /v ) glycerol, 0.5 mM DTT].

    Polymerase Chain Reaction:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The PCR product generated using the forward primer 5′-GCCCTCACCACCGCC CAG ACCACCGGTACCCAC-3′ and the reverse primer 5′-GTGGGTACCGGTGGT CTG GGCGGTGGTGAGGGC-3′ was treated with the DpnI restriction enzyme (Biolabs) to digest the methylated template plasmid. .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Generated:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The PCR product generated using the forward primer 5′-GCCCTCACCACCGCC CAG ACCACCGGTACCCAC-3′ and the reverse primer 5′-GTGGGTACCGGTGGT CTG GGCGGTGGTGAGGGC-3′ was treated with the DpnI restriction enzyme (Biolabs) to digest the methylated template plasmid. .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    DNA Sequencing:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen). .. Plasmid DNA was extracted (QIAprep Spin Miniprep Kit, Qiagen) and verified by DNA sequencing (DNA Sequencing Unit, University of Dundee).

    CTG Assay:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The PCR product generated using the forward primer 5′-GCCCTCACCACCGCC CAG ACCACCGGTACCCAC-3′ and the reverse primer 5′-GTGGGTACCGGTGGT CTG GGCGGTGGTGAGGGC-3′ was treated with the DpnI restriction enzyme (Biolabs) to digest the methylated template plasmid. .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen).

    Over Expression:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen). .. Overexpression and protein purification were performed as described for wild-type Pa FabF but using phosphate buffer [50 mM Na2 HPO4 pH 7.8, 300 mM NaCl, 10%(v /v ) glycerol, 0.5 mM DTT].

    Plasmid Preparation:

    Article Title: Structures of Pseudomonas aeruginosa β-ketoacyl-(acyl-carrier-protein) synthase II (FabF) and a C164Q mutant provide templates for antibacterial drug discovery and identify a buried potassium ion and a ligand-binding site that is an artefact of the crystal form
    Article Snippet: .. The Pa FabF-C164Q encoding plasmid was amplified by transforming E. coli XL1-Blue supercompetent cells (Novagen). .. Plasmid DNA was extracted (QIAprep Spin Miniprep Kit, Qiagen) and verified by DNA sequencing (DNA Sequencing Unit, University of Dundee).