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Agilent technologies e coli xl1 blue supercompetent cells
E Coli Xl1 Blue Supercompetent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli xl1 blue supercompetent cells/product/Agilent technologies
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
e coli xl1 blue supercompetent cells - by Bioz Stars, 2020-04
94/100 stars

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Amplification:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen). .. The mutations were verified by DNA sequencing.

Modification:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: For both mutants, the following primers were designed to introduce the desired mutations: AIM-1-Q157N-F (5′-GACCGCACCGACCCG AAC TTCGAGGTGGCCGAAC-3′) and AIM-1-Q157N-R (5′-GTTCGGCCACCTCGAA GTT CGGGTCGGTGCGGTC-3′) for the Q157N mutant and AIM-1-Q157A-F (5′-CCGCACCGACCCG GC ATTCGAGGTGGC-3′) and AIM-1-Q157A-R (5′ GCCACCTCGAAT GC CGGGTCGGTGCGG-3′) for the Q157A mutant (the modified bases are underlined). .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen).

Mutagenesis:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen). .. The mutations were verified by DNA sequencing.

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: The E310A mutant was introduced into pCW-4B1#7 plasmid using the QuikChange® II site-directed mutagenesis kit (Agilent). .. Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent).

Introduce:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: For both mutants, the following primers were designed to introduce the desired mutations: AIM-1-Q157N-F (5′-GACCGCACCGACCCG AAC TTCGAGGTGGCCGAAC-3′) and AIM-1-Q157N-R (5′-GTTCGGCCACCTCGAA GTT CGGGTCGGTGCGGTC-3′) for the Q157N mutant and AIM-1-Q157A-F (5′-CCGCACCGACCCG GC ATTCGAGGTGGC-3′) and AIM-1-Q157A-R (5′ GCCACCTCGAAT GC CGGGTCGGTGCGG-3′) for the Q157A mutant (the modified bases are underlined). .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen).

Purification:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen). .. The mutations were verified by DNA sequencing.

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: Paragraph title: Protein expression and purification ... Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent).

Polymerase Chain Reaction:

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: .. Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent). .. The sequence of the mutant construct was confirmed by DNA sequencing, and the coding sequence was excised with NdeI and SalI and subcloned into pCW expression vector.

Construct:

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent). .. The sequence of the mutant construct was confirmed by DNA sequencing, and the coding sequence was excised with NdeI and SalI and subcloned into pCW expression vector.

Expressing:

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: Paragraph title: Protein expression and purification ... Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent).

Sequencing:

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: The forward mutagenic primer sequence is 5′-GACACGTTCATGTTCG C AGGTCATGACACCAC-3′ with the altered nucleotide underlined. .. Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent).

Transformation Assay:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen). .. The mutations were verified by DNA sequencing.

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: .. Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent). .. The sequence of the mutant construct was confirmed by DNA sequencing, and the coding sequence was excised with NdeI and SalI and subcloned into pCW expression vector.

Plasmid Preparation:

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: .. Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent). .. The sequence of the mutant construct was confirmed by DNA sequencing, and the coding sequence was excised with NdeI and SalI and subcloned into pCW expression vector.

DNA Sequencing:

Article Title: Crystal Structure of the Mobile Metallo-?-Lactamase AIM-1 from Pseudomonas aeruginosa: Insights into Antibiotic Binding and the Role of Gln157
Article Snippet: Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen). .. Amplified DNA from the mutagenesis reaction was digested with DpnI to cleave the parental DNA (nonmutated), purified, and transformed into E. coli XL1-Blue supercompetent cells (Agilent) and subsequently into E. coli BL21(DE3) (Invitrogen).

Article Title: Noncovalent interactions dominate dynamic heme distortion in cytochrome P450 4B1
Article Snippet: Following PCR, the PCR mixture was digested with DpnI to remove the plasmid template and then transformed into E. coli XL1-Blue supercompetent cells (Agilent). .. The sequence of the mutant construct was confirmed by DNA sequencing, and the coding sequence was excised with NdeI and SalI and subcloned into pCW expression vector.

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    Agilent technologies e coli xl1 blue supercompetent cells
    E Coli Xl1 Blue Supercompetent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue supercompetent cells/product/Agilent technologies
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue supercompetent cells - by Bioz Stars, 2020-04
    94/100 stars
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