Structured Review

Stratagene e coli xl1 blue mrf host cells
E Coli Xl1 Blue Mrf Host Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli xl1 blue mrf host cells/product/Stratagene
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
e coli xl1 blue mrf host cells - by Bioz Stars, 2020-04
84/100 stars

Related Products / Commonly Used Together

twenty 150-mm petri dishes

Images

Related Articles

Clone Assay:

Article Title: Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences †
Article Snippet: Phage plaques were allowed to form for 3 h at 42°C on Escherichia coli XL1-Blue MRF′ host cells (Stratagene) grown on 150-mm NZY agar plates. .. Antigenic cDNA clones were identified by screening the filters with cerebrospinal fluid (CSF) from a horse that had been naturally infected with S. neurona and exhibited a high titer of intrathecal antibodies against S. neurona in a Western blot analysis.

Amplification:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: .. The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C. ..

Isolation:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: Paragraph title: Isolation of the P37 gene clone. ... The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C.

Infection:

Article Title: Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences †
Article Snippet: Phage plaques were allowed to form for 3 h at 42°C on Escherichia coli XL1-Blue MRF′ host cells (Stratagene) grown on 150-mm NZY agar plates. .. Antigenic cDNA clones were identified by screening the filters with cerebrospinal fluid (CSF) from a horse that had been naturally infected with S. neurona and exhibited a high titer of intrathecal antibodies against S. neurona in a Western blot analysis.

Purification:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: Total DNA was purified from cultured B. burgdorferi cells as previously described ( ). .. The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C.

Incubation:

Article Title: Bacterial flagellin is a dominant antigen in Crohn disease
Article Snippet: .. Briefly, twenty 150-mm petri dishes were plated with E. coli XL1-Blue MRF′ host cells (Stratagene) and approximately 3 ∞ 104 PFU of the unamplified library and were incubated at 42°C until plaques formed. .. Dry nitrocellulose filters (Schleicher and Schuell, Keene, New Hampshire, USA), pre-wet with 10 mM isopropyl β-thiogalactopyranoside (IPTG), were placed on the plates, which were then incubated overnight at 37°C.

Article Title: Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences †
Article Snippet: Phage plaques were allowed to form for 3 h at 42°C on Escherichia coli XL1-Blue MRF′ host cells (Stratagene) grown on 150-mm NZY agar plates. .. When plaques became visible, the agar was overlaid with nitrocellulose filters previously soaked in 10 mM isopropyl-β- d -thiogalactopyranoside (IPTG), and the plates were incubated for an additional 3 h at 37°C.

Cell Culture:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: Total DNA was purified from cultured B. burgdorferi cells as previously described ( ). .. The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C.

Expressing:

Article Title: Bacterial flagellin is a dominant antigen in Crohn disease
Article Snippet: Paragraph title: Expression screening. ... Briefly, twenty 150-mm petri dishes were plated with E. coli XL1-Blue MRF′ host cells (Stratagene) and approximately 3 ∞ 104 PFU of the unamplified library and were incubated at 42°C until plaques formed.

Western Blot:

Article Title: Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences †
Article Snippet: Phage plaques were allowed to form for 3 h at 42°C on Escherichia coli XL1-Blue MRF′ host cells (Stratagene) grown on 150-mm NZY agar plates. .. Antigenic cDNA clones were identified by screening the filters with cerebrospinal fluid (CSF) from a horse that had been naturally infected with S. neurona and exhibited a high titer of intrathecal antibodies against S. neurona in a Western blot analysis.

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C. .. A serum sample from a Lyme disease patient that had a strong IgG response to the P37 antigen, as demonstrated by Western blotting, was selected.

cDNA Library Assay:

Article Title: Sarcocystis neurona Merozoites Express a Family of Immunogenic Surface Antigens That Are Orthologues of the Toxoplasma gondii Surface Antigens (SAGs) and SAG-Related Sequences †
Article Snippet: Paragraph title: Immunoscreening of S. neurona cDNA library. ... Phage plaques were allowed to form for 3 h at 42°C on Escherichia coli XL1-Blue MRF′ host cells (Stratagene) grown on 150-mm NZY agar plates.

Construct:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: A genomic DNA library of B. burgdorferi B31 (low passage, < 10 passages) was constructed in the phage lambda vector ZapExpress (Stratagene, La Jolla, Calif.) as follows. .. The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C.

Plasmid Preparation:

Article Title: The Borrelia burgdorferi 37-Kilodalton Immunoblot Band (P37) Used in Serodiagnosis of Early Lyme Disease Is the flaA Gene Product
Article Snippet: A genomic DNA library of B. burgdorferi B31 (low passage, < 10 passages) was constructed in the phage lambda vector ZapExpress (Stratagene, La Jolla, Calif.) as follows. .. The phage library was plated onto E. coli XL1-Blue MRF′ host cells (Stratagene) and amplified, titers were determined, and the library was stored at 4°C.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Stratagene escherichia coli xl1 blue mrf host cells
    Escherichia Coli Xl1 Blue Mrf Host Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli xl1 blue mrf host cells/product/Stratagene
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli xl1 blue mrf host cells - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results