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Agilent technologies e coli xl1 blue electroporation competent cells
E Coli Xl1 Blue Electroporation Competent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli xl1 blue electroporation competent cells/product/Agilent technologies
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
e coli xl1 blue electroporation competent cells - by Bioz Stars, 2020-04
90/100 stars

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Electroporation:

Article Title: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1
Article Snippet: .. The resulting phagemid DNA from the Fab fragment library construction was transformed into E. coli XL1-Blue electroporation competent cells (Agilent Technologies). .. The volume of the cultivation was increased with Super Broth (SB) medium supplemented with carbenicillin (20 μg/ml) and tetracycline (10 μg/ml) and incubated for an hour at 37°C with agitation followed by a second supplementation with carbenicillin (50 μg/ml).

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol. .. Clones containing the EFG1 gene with the SAT1 - CamR cassette were selected on LB plates containing 34 μg/ml chloramphenicol.

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. Plasmid DNA was isolated by E. coli DNA miniprep as previously described .

Transfection:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. To re-evaluate the suppressor activity of the identified candidate gene, suppressor plasmid was transfected to yeast again and performed spot assay.

Selection:

Article Title: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1
Article Snippet: Paragraph title: Selection of the mouse IgG antibody libraries ... The resulting phagemid DNA from the Fab fragment library construction was transformed into E. coli XL1-Blue electroporation competent cells (Agilent Technologies).

Agarose Gel Electrophoresis:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: PCR product was digested with EcoRI and HindIII for 1 h at 37 °C and separated by 1% agarose gel electrophoresis. .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Clone Assay:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: Paragraph title: Cloning EFG1 from C. tropicalis ... Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Isolation:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. Plasmid DNA was isolated by E. coli DNA miniprep as previously described .

Ligation:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol. .. Clones containing the EFG1 gene with the SAT1 - CamR cassette were selected on LB plates containing 34 μg/ml chloramphenicol.

Construct:

Article Title: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1
Article Snippet: The selection of rMal d 1 and rApi g 1-specific antibodies from the constructed Fab fragment libraries was based on the specific protein-protein interactions. .. The resulting phagemid DNA from the Fab fragment library construction was transformed into E. coli XL1-Blue electroporation competent cells (Agilent Technologies).

Sequencing:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: Transformants with the correct insertion at the center of the endogenous EFG1 sequence were selected on YEPD plates containing 400 μg/ml of nourseothricin and verified by colony PCR. .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Relatively big colonies were picked up and subjected to colony PCR using primers 5′-AAGATCAACACTGCCAAAGCG-3′ and 5′-AGCCATATCAATACGGCGAACATC-3′ to amplify DNA sequence of gene inserted in plasmid. .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Polymerase Chain Reaction:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: Transformants with the correct insertion at the center of the endogenous EFG1 sequence were selected on YEPD plates containing 400 μg/ml of nourseothricin and verified by colony PCR. .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: PCR product was digested with EcoRI and HindIII for 1 h at 37 °C and separated by 1% agarose gel electrophoresis. .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Incubation:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: The cell suspension in each well was plated onto SC-His agar plate and incubated for 3 days at 30 °C. .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Amplification:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: Based on the sequence of the amplified fragment and conserved regions further out on the C. albicans gene, more primer pairs were designed to further extend the amplified region by PCR ( Table S1 ). .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Activity Assay:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. To re-evaluate the suppressor activity of the identified candidate gene, suppressor plasmid was transfected to yeast again and performed spot assay.

Cell Culture:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: The 8 groups of transformants were cultivated in SC-His medium until mid-log phase, seeded to 96 well-plate and cultured in YPD liquid medium with 100 μM BITC for 3 days at 30 °C. .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Marker:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: These two regions were PCR-fused to the edges of a segment containing the SAT1 marker, the CamR marker, and an E. coli origin of replication that was PCR-amplified from plasmid pSFS2A ( ). .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Transformation Assay:

Article Title: Isolation of Mal d 1 and Api g 1 - specific recombinant antibodies from mouse IgG Fab fragment libraries – Mal d 1-specific antibody exhibits cross-reactivity against Bet v 1
Article Snippet: .. The resulting phagemid DNA from the Fab fragment library construction was transformed into E. coli XL1-Blue electroporation competent cells (Agilent Technologies). .. The volume of the cultivation was increased with Super Broth (SB) medium supplemented with carbenicillin (20 μg/ml) and tetracycline (10 μg/ml) and incubated for an hour at 37°C with agitation followed by a second supplementation with carbenicillin (50 μg/ml).

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: The resulting cassette (5′ EFG1 homology—drug markers and origin of replication—3′ EFG1 homology) was transformed into C. tropicalis cells as previously described. .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. Plasmid DNA was isolated by E. coli DNA miniprep as previously described .

Over Expression:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Paragraph title: Screening for overexpression suppressors of antiproliferation by BITC in yeast ... Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

Spot Test:

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate. .. To re-evaluate the suppressor activity of the identified candidate gene, suppressor plasmid was transfected to yeast again and performed spot assay.

Plasmid Preparation:

Article Title: Finding a Missing Gene: EFG1 Regulates Morphogenesis in Candida tropicalis
Article Snippet: These two regions were PCR-fused to the edges of a segment containing the SAT1 marker, the CamR marker, and an E. coli origin of replication that was PCR-amplified from plasmid pSFS2A ( ). .. Genomic DNA from the transformants was extracted, digested with Xho I and Nhe I separately, circularized by ligation, and electroporated into E. coli XL1-Blue Electroporation-Competent Cells (Agilent Technologies) following the manufacturer’s protocol.

Article Title: Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12
Article Snippet: Plasmid DNA from colony with the same band pattern frequently seen here (more than 2 times) was isolated by Yeast DNA miniprep as previously described . .. Escherichia coli XL1-Blue Electroporation Competent Cell (Agilent Technologies, Santa Clara, CA) was transformed with the isolated yeast DNA by electroporation and plated on ampicillin-containing LB plate.

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    Agilent technologies e coli xl1 blue electroporation competent cells
    E Coli Xl1 Blue Electroporation Competent Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue electroporation competent cells/product/Agilent technologies
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue electroporation competent cells - by Bioz Stars, 2020-04
    90/100 stars
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