e coli xl1 blue cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e coli xl1 blue cells
    E Coli Xl1 Blue Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli xl1 blue cells/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli xl1 blue cells - by Bioz Stars, 2020-04
    85/100 stars

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    Related Articles

    Electroporation Bacterial Transformation:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, a minimum of 32 clones was selected, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Clone Assay:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, a minimum of 32 clones was selected, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs). ..

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs). ..

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen). .. Positive entry clones were confirmed by colony PCR screening with primers M13Forward and M13Reverse which bind to internal vector sequences adjacent to the att sites.

    Centrifugation:

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3. .. Protein expression was induced with 1 mM isopropyl 1-thio- d -galactopyranoside (Fermentas AB, Vilnius, Lithuania), followed by further incubation for 4 h. Cells were harvested by centrifugation, were resuspended in solution A (100 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM KCl, 10% glycerol, 0.5% Tween 20, 1 mM aminocaproic acid, and 1 mM phenylmethylsulfonyl fluoride) supplemented with 100 mg of lysozyme per milliliter (Sigma-Aldrich), and were incubated at 4°C for 30 min.

    Amplification:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: The amplification of BTN3A3Like alleles was only successful by using a specific exon 2 5′primer and an exon 4 3′primer (Suppl. .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: FDX2 was amplified from human FDX2 cDNA cloned in pCMV-SPORT6 (Dharmacon). .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: FDX2 was amplified from human FDX2 cDNA cloned in pCMV-SPORT6 (Dharmacon). .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Mutagenesis:

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs). ..

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs). ..

    Isolation:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: Paragraph title: RNA isolation, cDNA preparation, cloning and sequencing ... The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    TA Cloning:

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Construct:

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: A pCMHIS vector was constructed by inserting the HIS3 marker between the Sfo I and Eco RI sites of pCM190. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: A pCM HIS vector was constructed by inserting the HIS3 marker between the Sfo I and Eco RI sites of pCM190. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: We therefore constructed three Gateway destination vectors for expression of target proteins fused to MBP-His7 , GST-His7 , and NusA-His6 (pD-MAL1, pD-GEX1, and pD-Nus1, respectively). .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen).

    Purification:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: Paragraph title: Expression and purification of recombinant PvNod22 ... E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: Paragraph title: Cloning, expression, and purification of VraR. ... The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: All three destination vectors contained the sequence coding for a His-tag, enabling purification of either protein fusion by His-tag affinity to Ni-NTA agarose. .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen).

    Sequencing:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: Paragraph title: RNA isolation, cDNA preparation, cloning and sequencing ... The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Paragraph title: Cloning and sequencing ... Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: All three destination vectors contained the sequence coding for a His-tag, enabling purification of either protein fusion by His-tag affinity to Ni-NTA agarose. .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen).

    Marker:

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: A pCMHIS vector was constructed by inserting the HIS3 marker between the Sfo I and Eco RI sites of pCM190. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: A pCM HIS vector was constructed by inserting the HIS3 marker between the Sfo I and Eco RI sites of pCM190. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Incubation:

    Article Title: AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis
    Article Snippet: N. meningitidis was grown on BHI media with 5% Levanthal's supplement, and incubated at 37°C in the presence of 5% CO2 . .. E. coli XL1-blue cells (Invitrogen, for recombinant work) and ER2566 (New England Biolabs), for protein expression, were propagated in LB media.

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3. .. Protein expression was induced with 1 mM isopropyl 1-thio- d -galactopyranoside (Fermentas AB, Vilnius, Lithuania), followed by further incubation for 4 h. Cells were harvested by centrifugation, were resuspended in solution A (100 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM KCl, 10% glycerol, 0.5% Tween 20, 1 mM aminocaproic acid, and 1 mM phenylmethylsulfonyl fluoride) supplemented with 100 mg of lysozyme per milliliter (Sigma-Aldrich), and were incubated at 4°C for 30 min.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The clones were incubated in Luria-Bertani (LB) broth with shaking at 35°C.

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen). .. In the LR reaction, plasmid preparations of entry clones were incubated with the destination vectors pD-MAL1, pD-GEX1, and pD-Nus1, respectively, in order to generate E. coli SCS1 expression clones for MBP-His7 or GST-His7 fusion proteins.

    DNA Sequencing:

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Expressing:

    Article Title: AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis
    Article Snippet: .. E. coli XL1-blue cells (Invitrogen, for recombinant work) and ER2566 (New England Biolabs), for protein expression, were propagated in LB media. ..

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: Paragraph title: Expression and purification of recombinant PvNod22 ... E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: We therefore constructed three Gateway destination vectors for expression of target proteins fused to MBP-His7 , GST-His7 , and NusA-His6 (pD-MAL1, pD-GEX1, and pD-Nus1, respectively). .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen).

    Gel Extraction:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Polymerase Chain Reaction:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen). .. Positive entry clones were confirmed by colony PCR screening with primers M13Forward and M13Reverse which bind to internal vector sequences adjacent to the att sites.

    Sonication:

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3. .. Cell lysate was centrifuged (30 min at 10,000 × g ), washed twice with solution A, and sonicated ( , lanes 1 and 4).

    Transformation Assay:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, a minimum of 32 clones was selected, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: .. E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3. .. Protein expression was induced with 1 mM isopropyl 1-thio- d -galactopyranoside (Fermentas AB, Vilnius, Lithuania), followed by further incubation for 4 h. Cells were harvested by centrifugation, were resuspended in solution A (100 mM Tris-HCl [pH 7.5], 100 mM NaCl, 10 mM KCl, 10% glycerol, 0.5% Tween 20, 1 mM aminocaproic acid, and 1 mM phenylmethylsulfonyl fluoride) supplemented with 100 mg of lysozyme per milliliter (Sigma-Aldrich), and were incubated at 4°C for 30 min.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™). .. Per animal, 24 to 48 bacterial clones were picked, and plasmid DNA was isolated using a standard mini-preparation procedure.

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen). .. Positive entry clones were confirmed by colony PCR screening with primers M13Forward and M13Reverse which bind to internal vector sequences adjacent to the att sites.

    Recombinant:

    Article Title: AP endonuclease paralogues with distinct activities in DNA repair and bacterial pathogenesis
    Article Snippet: .. E. coli XL1-blue cells (Invitrogen, for recombinant work) and ER2566 (New England Biolabs), for protein expression, were propagated in LB media. ..

    Article Title: Nodulin 22, a Novel Small Heat-Shock Protein of the Endoplasmic Reticulum, Is Linked to the Unfolded Protein Response in Common Bean
    Article Snippet: Paragraph title: Expression and purification of recombinant PvNod22 ... E. coli XL1-Blue cells transformed with pQE30- PvNod 22 ( ) were grown in Luria Bertani broth (Gibco BRL) medium containing 100 μg of ampicillin per milliliter at 37°C until adsorbance at 600 nm reached 0.3.

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Plasmid Preparation:

    Article Title: Analysis of macaque BTN3A genes and transcripts in the extended MHC: conserved orthologs of human γδ T cell modulators
    Article Snippet: A final extension step was performed at 72 °C for 7 min. PCR products were purified using a geneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. The cloned amplicons were then transformed in Escherichia coli -XL1-blue cells with the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: VraR Binding to the Promoter Region of agr Inhibits Its Function in Vancomycin-Intermediate Staphylococcus aureus (VISA) and Heterogeneous VISA
    Article Snippet: .. The amplified product was cloned into pGEM-T vector (Promega) using the TA cloning procedure and transformed into Escherichia coli XL1-Blue cells (Invitrogen). vraR was subcloned into the expression vector pET28a (Invitrogen), and the recombinant plasmid was transformed into E. coli BL21(DE3). .. The transformants were checked for the insert by using colony PCR and DNA sequencing.

    Article Title: Determining Mhc-DRB profiles in wild populations of three congeneric true lemur species by noninvasive methods
    Article Snippet: Cloning and sequencing PCR products were purified using a GeneJet Gel Extraction Kit (Thermo Scientific™), and the purified amplicons were cloned into the pJET vector using the CloneJET PCR cloning kit, both according to the manufacturer’s guidelines (Thermo Scientific™). .. Next, the cloned amplicons were transformed in Escherichia coli XL1-blue cells by using the TransformAid Bacterial Transformation Kit (Thermo Scientific™).

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: This plasmid and the pCMHIS empty-vector were used to transform the mxr1Δ /mxr2Δ strain. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Mitochondrial Ferredoxin Determines Vulnerability of Cells to Copper Excess
    Article Snippet: This plasmid and the pCM HIS empty-vector were used to transform the mxr1Δ / mxr2Δ strain. .. All DNA cloning and genetic manipulations were in Escherichia coli XL1-Blue cells (Invitrogen) or high-efficiency NEB 5-alpha competent E. coli for mutagenesis (New England BioLabs).

    Article Title: Homologous high-throughput expression and purification of highly conserved E coli proteins
    Article Snippet: .. In order to create entry clones, we performed BP recombination reactions between pQTEV2 containing the gene of interest and the Gateway donor vector pDONR221, followed by the transformation of E. coli XL1-blue cells, according to the manufacturer's instructions (Invitrogen). .. Positive entry clones were confirmed by colony PCR screening with primers M13Forward and M13Reverse which bind to internal vector sequences adjacent to the att sites.

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    Thermo Fisher e coli xl1 blue competent cells
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