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TaKaRa e coli stellar
E Coli Stellar, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli stellar/product/TaKaRa
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
e coli stellar - by Bioz Stars, 2020-07
99/100 stars

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Molecular Cloning:

Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

Amplification:

Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

Article Title: The Different Effects of Substrates and Nucleotides on the Complex Formation of ABC Transporters
Article Snippet: .. All plasmids were amplified by transforming them into E. coli Stellar Competent Cells (Takara) and the DNA sequences were verified by Sanger sequencing. .. For each protein, the plasmid was transformed in E. coli BL21(DE3) (New England Biolabs).

Clone Assay:

Article Title: Analysis of Recombinant H7N9 Wild-Type and Mutant Viruses in Pigs Shows that the Q226L Mutation in HA Is Important for Transmission
Article Snippet: .. The synthesized segments ( ) were cloned into the modified bidirectional influenza virus reverse genetics vectors pBZ61A18 (PB2, PB1, and PA) and pBZ61A15 (HA, NP, NA, M, and NS) using the recombination-based method ( ) and transformed into Stella-competent Escherichia coli cells (Clontech). ..

Article Title: HAfTs are novel lncRNA transcripts from aflatoxin exposure
Article Snippet: .. RACE cycling conditions were for 25 cycles at 94°C for 30 sec, 68°C for 30 sec, and 72°C for 3 min. In-Fusion® directional cloning of RACE products was performed using Stellar™ competent E . coli (Clontech) prior to Sanger sequencing. ..

Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

Modification:

Article Title: Analysis of Recombinant H7N9 Wild-Type and Mutant Viruses in Pigs Shows that the Q226L Mutation in HA Is Important for Transmission
Article Snippet: .. The synthesized segments ( ) were cloned into the modified bidirectional influenza virus reverse genetics vectors pBZ61A18 (PB2, PB1, and PA) and pBZ61A15 (HA, NP, NA, M, and NS) using the recombination-based method ( ) and transformed into Stella-competent Escherichia coli cells (Clontech). ..

Synthesized:

Article Title: Analysis of Recombinant H7N9 Wild-Type and Mutant Viruses in Pigs Shows that the Q226L Mutation in HA Is Important for Transmission
Article Snippet: .. The synthesized segments ( ) were cloned into the modified bidirectional influenza virus reverse genetics vectors pBZ61A18 (PB2, PB1, and PA) and pBZ61A15 (HA, NP, NA, M, and NS) using the recombination-based method ( ) and transformed into Stella-competent Escherichia coli cells (Clontech). ..

Size-exclusion Chromatography:

Article Title: HAfTs are novel lncRNA transcripts from aflatoxin exposure
Article Snippet: .. RACE cycling conditions were for 25 cycles at 94°C for 30 sec, 68°C for 30 sec, and 72°C for 3 min. In-Fusion® directional cloning of RACE products was performed using Stellar™ competent E . coli (Clontech) prior to Sanger sequencing. ..

Construct:

Article Title: Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin
Article Snippet: .. Cutinase expression constructs, in the pET30f vector [ ], was used to transform to E. coli Stellar™ Competent Cells (Clontech Laboratories, Mountain View, CA). .. Positive transformants were verified using colony PCR, restriction digestion and DNA sequencing analysis.

Polymerase Chain Reaction:

Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

Expressing:

Article Title: Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin
Article Snippet: .. Cutinase expression constructs, in the pET30f vector [ ], was used to transform to E. coli Stellar™ Competent Cells (Clontech Laboratories, Mountain View, CA). .. Positive transformants were verified using colony PCR, restriction digestion and DNA sequencing analysis.

Sequencing:

Article Title: HAfTs are novel lncRNA transcripts from aflatoxin exposure
Article Snippet: .. RACE cycling conditions were for 25 cycles at 94°C for 30 sec, 68°C for 30 sec, and 72°C for 3 min. In-Fusion® directional cloning of RACE products was performed using Stellar™ competent E . coli (Clontech) prior to Sanger sequencing. ..

Article Title: The Different Effects of Substrates and Nucleotides on the Complex Formation of ABC Transporters
Article Snippet: .. All plasmids were amplified by transforming them into E. coli Stellar Competent Cells (Takara) and the DNA sequences were verified by Sanger sequencing. .. For each protein, the plasmid was transformed in E. coli BL21(DE3) (New England Biolabs).

Transformation Assay:

Article Title: Analysis of Recombinant H7N9 Wild-Type and Mutant Viruses in Pigs Shows that the Q226L Mutation in HA Is Important for Transmission
Article Snippet: .. The synthesized segments ( ) were cloned into the modified bidirectional influenza virus reverse genetics vectors pBZ61A18 (PB2, PB1, and PA) and pBZ61A15 (HA, NP, NA, M, and NS) using the recombination-based method ( ) and transformed into Stella-competent Escherichia coli cells (Clontech). ..

Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

Article Title: Molecular elements in FGF19 and FGF21 defining KLB/FGFR activity and specificity
Article Snippet: .. Stellar E. coli competent cells (Clontech) were transformed to amplify the plasmid, which was harvested with QIA Miniprep kit (Qiagen). .. The positive clones isolated by LB/Ampicillin agar plate selection were confirmed by DNA sequencing and OrigamiB (DE3) E.coli cells (Novagen) for FGF21/19/23 analogs and BL21 (DE3) E. coli cells (Novagen) for FGF1/2 analogs were transformed for protein expression.

Plasmid Preparation:

Article Title: Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei
Article Snippet: .. 636763,Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) were used for plasmid construction and amplification using standard molecular cloning techniques (Sambrook and Russell, ). .. Generation of gene replacement cassettes To express fluorescently labeled transcription factors XYR1 and CRE1 from their native loci, we choose a simultaneous knock-out/knock-in strategy.

Article Title: A SNARE-Like Protein and Biotin Are Implicated in Soybean Cyst Nematode Virulence
Article Snippet: .. The resulting PCR product was treated with cloning enhancer, the plasmid was circularized using the In-Fusion HD enzyme premix and was transformed into E . coli Stellar competent cells following the manufacturers protocol (Clontech). .. The resulting plasmid containing HgSLP-1 missing the signal peptide was verified for accuracy by sequencing the nematode gene as described above.

Article Title: The “speed gene” effect of myostatin arises in Thoroughbred horses due to a promoter proximal SINE insertion
Article Snippet: .. The In-Fusion cloning was performed as per manufacturers’ recommendations and the plasmid was transformed into StellarTM competent cells (TaKaRa-Clontech) and was grown at 37°C. .. Subsequently, the plasmid DNA was isolated and sequenced using M13 primers ( 5'-TGT AAA ACG ACG GCC AGT-3' (forward), 5'-CAG GAA ACA GCT ATG ACC-3' (reverse)) by MWG eurofins, to ascertain where the transcription start site was on the sequence, as the inserted fragment sequence would begin at the transcription start site, preceded by the universal primer sequence.

Article Title: Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin
Article Snippet: .. Cutinase expression constructs, in the pET30f vector [ ], was used to transform to E. coli Stellar™ Competent Cells (Clontech Laboratories, Mountain View, CA). .. Positive transformants were verified using colony PCR, restriction digestion and DNA sequencing analysis.

Article Title: Molecular elements in FGF19 and FGF21 defining KLB/FGFR activity and specificity
Article Snippet: .. Stellar E. coli competent cells (Clontech) were transformed to amplify the plasmid, which was harvested with QIA Miniprep kit (Qiagen). .. The positive clones isolated by LB/Ampicillin agar plate selection were confirmed by DNA sequencing and OrigamiB (DE3) E.coli cells (Novagen) for FGF21/19/23 analogs and BL21 (DE3) E. coli cells (Novagen) for FGF1/2 analogs were transformed for protein expression.

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    TaKaRa escherichia coli stellar cells
    Escherichia Coli Stellar Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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