e coli o104 h4 strains ec 01  (ATCC)


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    ATCC e coli o104 h4 strains ec 01
    E Coli O104 H4 Strains Ec 01, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    e coli o104 h4 strains ec 01  (ATCC)


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    ATCC e coli o104 h4 strains ec 01
    <t> E. coli </t> strains used in the present study.
    E Coli O104 H4 Strains Ec 01, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli o104 h4 strains ec 01/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli o104 h4 strains ec 01 - by Bioz Stars, 2024-09
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    1) Product Images from "Shiga-Toxin-Producing Strains of Escherichia coli O104:H4 and a Strain of O157:H7, Which Can Cause Human Hemolytic Uremic Syndrome, Differ in Biofilm Formation in the Presence of CO 2 and in Their Ability to Grow in a Novel Cell Culture Medium"

    Article Title: Shiga-Toxin-Producing Strains of Escherichia coli O104:H4 and a Strain of O157:H7, Which Can Cause Human Hemolytic Uremic Syndrome, Differ in Biofilm Formation in the Presence of CO 2 and in Their Ability to Grow in a Novel Cell Culture Medium

    Journal: Microorganisms

    doi: 10.3390/microorganisms11071744

    E. coli strains used in the present study.
    Figure Legend Snippet: E. coli strains used in the present study.

    Techniques Used:

    CO 2 enhances biofilm formation by the E. coli O104:H4 strains but not the E. coli O157:H7 strains. The E. coli strains that were examined were E. coli O104:H4 (strains Ec01, Ec02, and Ec03), ATCC 25922, and O157:H7. The cultures were incubated in MH II broth overnight at 37 °C. All strains were examined in triplicate, and figures represent 1 of 3 separate studies. Only a single well of the triplicate is shown ( A , B ). A. Biofilm formation by the E. coli strains in a 96-well plate with and without CO 2 . B. Biofilm formation by the E. coli strains in 5 mL culture tubes with and without CO 2 . ( C ) Quantitative crystal violet analysis of the amount of biofilm formed by the E. coli strains in 5 mL culture tubes with and without CO 2 . In ( C ), results are presented as geometric mean with standard error of the mean. p -values are shown for the comparison of the amount of biofilm formation by E. coli O157:H7 with (orange line) and without (blue line) CO 2 and the amount of biofilm formation by the O104:H4 strains under the same conditions. p -values, when significant, are also shown for comparison of biofilm formation between individual strains with and without CO 2 .
    Figure Legend Snippet: CO 2 enhances biofilm formation by the E. coli O104:H4 strains but not the E. coli O157:H7 strains. The E. coli strains that were examined were E. coli O104:H4 (strains Ec01, Ec02, and Ec03), ATCC 25922, and O157:H7. The cultures were incubated in MH II broth overnight at 37 °C. All strains were examined in triplicate, and figures represent 1 of 3 separate studies. Only a single well of the triplicate is shown ( A , B ). A. Biofilm formation by the E. coli strains in a 96-well plate with and without CO 2 . B. Biofilm formation by the E. coli strains in 5 mL culture tubes with and without CO 2 . ( C ) Quantitative crystal violet analysis of the amount of biofilm formed by the E. coli strains in 5 mL culture tubes with and without CO 2 . In ( C ), results are presented as geometric mean with standard error of the mean. p -values are shown for the comparison of the amount of biofilm formation by E. coli O157:H7 with (orange line) and without (blue line) CO 2 and the amount of biofilm formation by the O104:H4 strains under the same conditions. p -values, when significant, are also shown for comparison of biofilm formation between individual strains with and without CO 2 .

    Techniques Used: Incubation, Comparison

    E. coli O104:H4 strains appear to grow in a novel cell culture medium but the E. coli O157:H7 strain does not. Live E. coli strains were examined for their ability to activate TLR2 or 4. The figure presents representative results from one of three studies we conducted with live bacterial strains after overnight incubation at 37 °C. ( A ) Bacterial strains and TLR control antigens were added in triplicate wells of HEK294 cells expressing TLR2 or 4 in an HBD cell culture medium overnight at 37 °C with 5% CO 2 in 96-well plates. Note the color reaction difference between the E. coli O104:H4 strains and the ATCC 25922 and O157:H7 strains. ( B ) The figure shows the amount of TLR2 or 4 activation by live E. coli strains. The results are presented as geometric means with standard errors of the mean. p -values are also shown comparing the amount of TLR2 activated by E. coli O157:H7 to the amount of TLR2 activated by the other E. coli strains (in blue). The differences between the results of TLR4 activation by E. coli O157:H7 and the other strains were not significant. Statistical comparisons are also shown between the amount of TLR2 and 4 activation of the individual E. coli strains. ( C ) Reaction using live E. coli strains with HEK293 cells not expressing TLR2 or 4 (null cells). ( D ) Incubation of live E. coli strains in HBD cell culture medium or DMEM cell culture maintenance medium without HEK293 cells.
    Figure Legend Snippet: E. coli O104:H4 strains appear to grow in a novel cell culture medium but the E. coli O157:H7 strain does not. Live E. coli strains were examined for their ability to activate TLR2 or 4. The figure presents representative results from one of three studies we conducted with live bacterial strains after overnight incubation at 37 °C. ( A ) Bacterial strains and TLR control antigens were added in triplicate wells of HEK294 cells expressing TLR2 or 4 in an HBD cell culture medium overnight at 37 °C with 5% CO 2 in 96-well plates. Note the color reaction difference between the E. coli O104:H4 strains and the ATCC 25922 and O157:H7 strains. ( B ) The figure shows the amount of TLR2 or 4 activation by live E. coli strains. The results are presented as geometric means with standard errors of the mean. p -values are also shown comparing the amount of TLR2 activated by E. coli O157:H7 to the amount of TLR2 activated by the other E. coli strains (in blue). The differences between the results of TLR4 activation by E. coli O157:H7 and the other strains were not significant. Statistical comparisons are also shown between the amount of TLR2 and 4 activation of the individual E. coli strains. ( C ) Reaction using live E. coli strains with HEK293 cells not expressing TLR2 or 4 (null cells). ( D ) Incubation of live E. coli strains in HBD cell culture medium or DMEM cell culture maintenance medium without HEK293 cells.

    Techniques Used: Cell Culture, Incubation, Control, Expressing, Activation Assay

    Differential growth of E. coli O104:H4 and O157:H7 strains in HBD cell culture medium. Single colonies of the E. coli O104:H4 or O157:H7 (and ATCC 25922) strains from SBA plates were used to inoculate 2 mL of MH II broth and cultures were incubated overnight at 37 °C. Overnight E. coli cultures were used to inoculate 12.5 mL of HBD cell culture medium (0.25 mL inoculum) or 12 mL MH II broth (0.12 mL inoculum) in 125 mL flasks and cultures were incubated at 37 °C with shaking at 180 rpm. Growth was followed by an increase in absorbance at 600 nm. The figure presents representative results from one of three independent studies. ( A ) Growth of the E . coli strains in HBD cell culture medium followed for 48 h. ( B ) Appearance of the E. coli cultures in HBD cell culture medium after 48 h. ( C ) Growth of the E. coli strains in MH II broth after 7 h of incubation.
    Figure Legend Snippet: Differential growth of E. coli O104:H4 and O157:H7 strains in HBD cell culture medium. Single colonies of the E. coli O104:H4 or O157:H7 (and ATCC 25922) strains from SBA plates were used to inoculate 2 mL of MH II broth and cultures were incubated overnight at 37 °C. Overnight E. coli cultures were used to inoculate 12.5 mL of HBD cell culture medium (0.25 mL inoculum) or 12 mL MH II broth (0.12 mL inoculum) in 125 mL flasks and cultures were incubated at 37 °C with shaking at 180 rpm. Growth was followed by an increase in absorbance at 600 nm. The figure presents representative results from one of three independent studies. ( A ) Growth of the E . coli strains in HBD cell culture medium followed for 48 h. ( B ) Appearance of the E. coli cultures in HBD cell culture medium after 48 h. ( C ) Growth of the E. coli strains in MH II broth after 7 h of incubation.

    Techniques Used: Cell Culture, Incubation

    Growth of E. coli O104:H4 strains in HBD cell culture medium starting with single colonies. A single colony from an overnight growth on SBA plates was used to inoculate 2 mL of HBD medium and cultures were incubated overnight at 37 °C with 5% CO 2 . The figure presents representative results from one of three independent studies. There were color differences between the E. coli O104:H4 strains and the ATCC 25922 and O157:H7 strains. This, along with the pH differences (pH ~5.0 vs. 8.5–9.0), reflected the growth of the E. coli O104:H4 strains. The purple color of the E. coli ATCC and O157:H7 cultures may have been derived from endogenous alkaline phosphatase that came from the original inoculum.
    Figure Legend Snippet: Growth of E. coli O104:H4 strains in HBD cell culture medium starting with single colonies. A single colony from an overnight growth on SBA plates was used to inoculate 2 mL of HBD medium and cultures were incubated overnight at 37 °C with 5% CO 2 . The figure presents representative results from one of three independent studies. There were color differences between the E. coli O104:H4 strains and the ATCC 25922 and O157:H7 strains. This, along with the pH differences (pH ~5.0 vs. 8.5–9.0), reflected the growth of the E. coli O104:H4 strains. The purple color of the E. coli ATCC and O157:H7 cultures may have been derived from endogenous alkaline phosphatase that came from the original inoculum.

    Techniques Used: Cell Culture, Incubation, Derivative Assay

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    ATCC e coli o104 h4 strains ec 01
    E Coli O104 H4 Strains Ec 01, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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