Structured Review

Stratagene e coli novablue
E Coli Novablue, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli novablue/product/Stratagene
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
e coli novablue - by Bioz Stars, 2020-05
92/100 stars

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Mutagenesis:

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Transformation Assay:

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Variant Assay:

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Expressing:

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

Article Title: Functional analysis of the Helicobacter pullorum N-linked protein glycosylation system
Article Snippet: .. A variant, pQEhgpAN46Q, was created by site directed mutagenesis using primers hgpAN46SDM-F and hgpAN46SDM-R. Plasmids pQEhgpA and pQEhgpAN46Q were transformed into E. coli Novablue (Stratagene) cells harboring pACYCpglB::aphA (24) along with plasmids expressing either C. jejuni pglB (pMAF10), H. pullorum pglB1 (pMLHp1) or H. pullorum pglB2 (pMLHp2) ( ). .. Transformants were grown overnight at 37°C, diluted 1 in 100 in LB broth supplemented with appropriate antibiotics, grown to an optical density at 600 nm (A600 ) of 0.6 and expression of hgpA his and pglB genes induced with 1 mM IPTG and 0.2% (w/v) arabinose.

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    Stratagene novablue de3 escherichia coli competent cells
    Novablue De3 Escherichia Coli Competent Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli novablue
    E Coli Novablue, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue/product/Stratagene
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli novablue - by Bioz Stars, 2020-05
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      Buy from Supplier

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