e coli novablue  (Millipore)


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    Name:
    Escherichia coli
    Description:

    Catalog Number:
    ec9637
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    Structured Review

    Millipore e coli novablue
    Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli <t>NovaBlue</t> plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus

    https://www.bioz.com/result/e coli novablue/product/Millipore
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    e coli novablue - by Bioz Stars, 2020-10
    99/100 stars

    Images

    1) Product Images from "Characterization of Fluorescent Chimeras of Cholera Toxin and Escherichia coli Heat-Labile Enterotoxins Produced by Use of the Twin Arginine Translocation System "

    Article Title: Characterization of Fluorescent Chimeras of Cholera Toxin and Escherichia coli Heat-Labile Enterotoxins Produced by Use of the Twin Arginine Translocation System

    Journal:

    doi: 10.1128/IAI.73.6.3627-3635.2005

    Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli NovaBlue plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus
    Figure Legend Snippet: Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli NovaBlue plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus

    Techniques Used: Fluorescence, Expressing, Incubation

    Related Articles

    Clone Assay:

    Article Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system
    Article Snippet: .. Cloning, co-expression and purification of the ExbBD complex The E. coli exbB and exbD genes were subcloned into vector pET-21b (Novagen). ..

    Flow Cytometry:

    Article Title: Antibodies Set Boundaries Limiting Microbial Metabolite Penetration and the Resultant Mammalian Host Response
    Article Snippet: .. Determining the extent of 13 C-labeling in E. coli HA107 used for stable isotope tracing The free intracellular metabolites were extracted from the cell pellet with Millipore water at 80°C and analyzed by non-targeted flow injection analysis as described above. ..

    Positron Emission Tomography:

    Article Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system
    Article Snippet: .. Cloning, co-expression and purification of the ExbBD complex The E. coli exbB and exbD genes were subcloned into vector pET-21b (Novagen). ..

    Polyacrylamide Gel Electrophoresis:

    Article Title: The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome
    Article Snippet: .. The mRNA analogues poly(U) and GAA-GAC-UAA-AAA, as well as E. coli tRNAPhe were purchased from Sigma-Aldrich. tRNAAsp from yeast was purified by conter-current chromatography, followed by separation by polyacrylamide gel electrophoresis. .. L-[14C(U)]Phenylalanine (18GBqxmmol-1) was from Perkin Elmer.

    Chromatography:

    Article Title: The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome
    Article Snippet: .. The mRNA analogues poly(U) and GAA-GAC-UAA-AAA, as well as E. coli tRNAPhe were purchased from Sigma-Aldrich. tRNAAsp from yeast was purified by conter-current chromatography, followed by separation by polyacrylamide gel electrophoresis. .. L-[14C(U)]Phenylalanine (18GBqxmmol-1) was from Perkin Elmer.

    Cell Culture:

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

    Purification:

    Article Title: The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome
    Article Snippet: .. The mRNA analogues poly(U) and GAA-GAC-UAA-AAA, as well as E. coli tRNAPhe were purchased from Sigma-Aldrich. tRNAAsp from yeast was purified by conter-current chromatography, followed by separation by polyacrylamide gel electrophoresis. .. L-[14C(U)]Phenylalanine (18GBqxmmol-1) was from Perkin Elmer.

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Article Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system
    Article Snippet: .. Cloning, co-expression and purification of the ExbBD complex The E. coli exbB and exbD genes were subcloned into vector pET-21b (Novagen). ..

    other:

    Article Title: Resistance to peroxynitrite in Neisseria gonorrhoeae
    Article Snippet: The ability of peroxynitrite to kill various gonococcal and meningococcal strains, as well as E. coli DH10B, was tested by directly adding peroxynitrite (Calbiochem) at final concentrations of 1 or 2 mM, or no addition, to exponential-phase cultures diluted into fresh GCK broth plus NaHCO3 .

    Article Title: Cardiac and hepatic role of r-AtHSP70: basal effects and protection against ischemic and sepsis conditions
    Article Snippet: LPS treatment To evaluate the possibility of r-AtHSP70 to counteract E. coli LPS (Sigma-Aldrich) -induced sepsis, animals were divided in three groups: (i ) treatment (i.p.) with a placebo of saline solution; (ii ) treatment by a single dose (i.p.) of LPS (5 mg/kgbw); (iii ) treatment with a single dose of LPS plus r-AtHSP70 (5 mg/kgbw).

    Expressing:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Lysis:

    Article Title: MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma
    Article Snippet: .. BL-21 E.Coli cells expressing MYCN-6×His were lysed in His lysis buffer (50 mM NaH2PO4, 1% Triton, 1 μg/μl lysozyme, 1 mM PMSF, 300 mM NaCl, and 20 mM imidazole), purified using HIS-Select Nickel Affinity Gel (Sigma Aldrich) and eluted with 350 mM imidazole in His lysis buffer without lysozyme. .. Purified MYCN-6×His protein was incubated with glutathione beads coated with equimolar amounts of either purified GST or GST-p53 overnight at 4°C.

    Injection:

    Article Title: Antibodies Set Boundaries Limiting Microbial Metabolite Penetration and the Resultant Mammalian Host Response
    Article Snippet: .. Determining the extent of 13 C-labeling in E. coli HA107 used for stable isotope tracing The free intracellular metabolites were extracted from the cell pellet with Millipore water at 80°C and analyzed by non-targeted flow injection analysis as described above. ..

    Plasmid Preparation:

    Article Title: Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants
    Article Snippet: .. For construction of E.coli expression plasmid pKN203 a Nde I– Bam HI full-length hDBR1 cDNA fragment from pKN201 was subcloned into Nde I and Bam HI digested pET28a (Novagen). .. The yeast expression plasmids pKN205 and pKN206 were constructed by inserting an Eco RI– Bam HI full-length hDBR1 cDNA fragment from pKN201 into S.cerevisiae expression vectors pRS314GU ( ) and pRS316GU ( ) digested with Eco RI and Bam HI, respectively.

    Article Title: Control of siRNA expression using the Cre-loxP recombination system
    Article Snippet: .. For residual E.coli lysate, pTriEx-3 Hygro empty vector was introduced into E.coli strain RosettaBlue(DE3)pLacI (Novagen) and cultured in LB medium that contained 1% glucose, 34 µg/ml chloramphenicol, 12.5 µg/ml tetracycline and 100 µg/ml ampicillin at 37°C until absorbance at 600 nm reached 0.7. .. After addition of IPTG to a final concentration of 1 mM, E.coli were harvested and treated in the same way as that of pTriEx-3 Hygro-TAT-NLS-Cre or pTriEx-3 Hygro-TAT-NLS-EGFP.

    Article Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system
    Article Snippet: .. Cloning, co-expression and purification of the ExbBD complex The E. coli exbB and exbD genes were subcloned into vector pET-21b (Novagen). ..

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