e coli novablue  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore e coli novablue
    E Coli Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue/product/Millipore
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    e coli novablue - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The PCR amplified insert was purified with SpinPrep Gel DNA Kit (Novagen, Darmstadt, Germany) and cloned via LIC into vector pET-32 Xa/LIC (EMD Millipore, Darmstadt, Germany). .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: .. Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) and JM109 ( ) were used as hosts for cloning. ..

    Article Title: S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
    Article Snippet: .. Escherichia coli NovaBlue and E. coli Rosetta(DE3) (Novagen, Madison, WI) were used as cloning and expression hosts, respectively. .. The E. coli strains were cultivated in Luria-Bertani (LB) medium, supplemented with kanamycin (Fisher Bioreagents) (30 μg/ml) and/or chloramphenicol (Sigma) (34 μg/ml).

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Article Title: IgG Endopeptidase SeMac does not Inhibit Opsonophagocytosis of Streptococcus equi Subspecies equi by Horse Polymorphonuclear Leukocytes
    Article Snippet: .. Escherichia coli Novablue and BL21(DE3) (Novagen, Madison, Wis.) were used for gene cloning and protein expression, respectively. .. S. equi and GAS strains were routinely grown in Todd-Hewitt broth (Difco Laboratories, Detroit, Mich.) supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C.

    Article Title: Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
    Article Snippet: .. E. coli NovaBlue (Novagen, Inc., Madison, WI, USA) was used as the cloning host for recombinant DNA manipulations. ..

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. Escherichia coli NovaBlue (Novagen) was used for gene cloning and BL21(DE3) (Novagen) was for GlmU protein expression. .. Cloning plasmid pJET1.2 blunt (Thermo) with the ampicillin resistance gene was used for cloning genes and re-sequencing the target genes.

    Amplification:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The PCR amplified insert was purified with SpinPrep Gel DNA Kit (Novagen, Darmstadt, Germany) and cloned via LIC into vector pET-32 Xa/LIC (EMD Millipore, Darmstadt, Germany). .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: The amplified fragments were digested with restriction enzymes and ligated into restriction enzyme–digested pET-33b(+) (Novagen). .. Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted.

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Thermal cycling conditions were: 95 °C for 3 min, followed by 32 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 1 min, and followed by a final incubation at 72 °C for 5 min. Amplification products were separated in 1.5% agarose gel in TAE buffer and stained with EthBr, then gel images were captured in a DNR Bio-Imaging System MF-ChemiBIS 2.0. (Mahale HaHamisha, Jerusalem, Israel) using Gel-Capture software. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: .. In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. Plasmid DNA was phenol–chloroform extracted, ethanol precipitated and resuspended in deionized and diethyl pyrocarbonate-treated water.

    Filtration:

    Article Title: Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
    Article Snippet: The mycelium in culture medium was separated from the culture medium by filtration using Miracloth (Calbiochem, Darmstadt, Germany) and was washed by distilled water. .. E. coli NovaBlue (Novagen, Inc., Madison, WI, USA) was used as the cloning host for recombinant DNA manipulations.

    TA Cloning:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Quantitative RT-PCR:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. .. Then, RT-qPCR was then performed using a thermal cycler StepOnePlus™ Real-Time PCR System (Applied Biosystems., Foster City, CA, USA).

    Incubation:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. For colony PCR, single colonies of screened positive clones were suspended in 50 µL of water, incubated at 99 °C for 5 min and centrifuged at 12,000× g for 1 min.

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Thermal cycling conditions were: 95 °C for 3 min, followed by 32 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 1 min, and followed by a final incubation at 72 °C for 5 min. Amplification products were separated in 1.5% agarose gel in TAE buffer and stained with EthBr, then gel images were captured in a DNR Bio-Imaging System MF-ChemiBIS 2.0. (Mahale HaHamisha, Jerusalem, Israel) using Gel-Capture software. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    Activity Assay:

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany). .. Sfβgly mutants were selected based on the activity upon 4 mM p-nitrophenyl β-glucoside (NPβglc) and 14 mM cellobiose.

    Expressing:

    Article Title: S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
    Article Snippet: .. Escherichia coli NovaBlue and E. coli Rosetta(DE3) (Novagen, Madison, WI) were used as cloning and expression hosts, respectively. .. The E. coli strains were cultivated in Luria-Bertani (LB) medium, supplemented with kanamycin (Fisher Bioreagents) (30 μg/ml) and/or chloramphenicol (Sigma) (34 μg/ml).

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: 2.1 Random mutagenesis and library screening A sample of the expression vector pCAL coding for the wild-type Sfβgly was submitted to random mutagenesis using the GeneMorph II kit (Agilent Technologies; Santa Clara, US) following the manufacturer's instructions. .. The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany).

    Article Title: IgG Endopeptidase SeMac does not Inhibit Opsonophagocytosis of Streptococcus equi Subspecies equi by Horse Polymorphonuclear Leukocytes
    Article Snippet: .. Escherichia coli Novablue and BL21(DE3) (Novagen, Madison, Wis.) were used for gene cloning and protein expression, respectively. .. S. equi and GAS strains were routinely grown in Todd-Hewitt broth (Difco Laboratories, Detroit, Mich.) supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C.

    Article Title: Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome
    Article Snippet: Plasmid Vectors, Bacterium Strains, and Growth Conditions The protein expression vector used was pETBlue-2. .. E. coli NovaBlue (Novagen) strain was used as the screening host for the pETBlue-2 bearing inserts.

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: .. Escherichia coli NovaBlue (Novagen) was used for gene cloning and BL21(DE3) (Novagen) was for GlmU protein expression. .. Cloning plasmid pJET1.2 blunt (Thermo) with the ampicillin resistance gene was used for cloning genes and re-sequencing the target genes.

    Transformation Assay:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. Positive clones were screened for via ampicillin and kanamycin resistance.

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: .. Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted. ..

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Conjugation Assay:

    Article Title: Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides
    Article Snippet: .. Escherichia coli NovaBlue (Novagen, Darmstadt, Germany) and S17-1 strains were used for DNA manipulation and conjugation transfer, respectively. ..

    Sequencing:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The gene insert was amplified using primers 5′-GGTATTGAGGGTCGCATATTATATTATTTA-3′ and 5′-AGAGGAGAGTTAGAGCCATAATAATATTTA-3′ (IDTDNA), which contained the requisite ligation-independent cloning (LIC); overhangs underlined in the sequence above. .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: The sequence of primers was as follows: IL-6 (sense) 5′-CTGCTCCTGGTGTTGCCT-3′, IL-6 (antisense) 5′-CTGAAGAGGTGAGTGGCTGT-3′; IL-8 (sense) 5′-ACTGAGAGTGATTGAGAGTGG-3′, IL-8 (antisense) 5′-AGTTTTCCTTGGGGTCCAGA-3′; CXCL1 (sense) 5′-GACCAGAAGGGAGGAGGAAG-3′, CXCL1 (antisense) 5′-ATGCTCAAACACATTAGGCACA-3′; CCL2 (sense) 5′-AAGCAGAAGTGGGTTCAGGATT-3′, CCL2 (antisense) 5′-TTGGGTTGTGGAGTGAGTGTT-3′; Beta actin (sense) 5′-GTTGCGTTACACCCTTTCTTG-3′, Beta actin (antisense) 5′-CACCTTCACCGTTCCAGTTT-3’. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    Ligation:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The gene insert was amplified using primers 5′-GGTATTGAGGGTCGCATATTATATTATTTA-3′ and 5′-AGAGGAGAGTTAGAGCCATAATAATATTTA-3′ (IDTDNA), which contained the requisite ligation-independent cloning (LIC); overhangs underlined in the sequence above. .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Library Screening:

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: Paragraph title: Random mutagenesis and library screening ... The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany).

    DNA Sequencing:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. Insertion was verified by colony PCR (EMD Millipore) and DNA sequencing.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Paragraph title: 4.6. RT-PCR and Quantitative Real-Time RT-PCR (RT-qPCR) ... In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    Recombinant:

    Article Title: Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae
    Article Snippet: .. Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) was used as the host strain for recombinant DNA manipulation. ..

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: Paragraph title: 3.1. Construction of Recombinant Plasmid ... The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae
    Article Snippet: Escherichia coli NovaBlue (Novagen, Darmstadt, Germany) strain was used for DNA manipulation. .. E. coli Rosetta™(DE3)pLysS (Novagen) strain was used to produce recombinant proteins.

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: Paragraph title: Recombinant Proteins ... Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted.

    Article Title: Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
    Article Snippet: .. E. coli NovaBlue (Novagen, Inc., Madison, WI, USA) was used as the cloning host for recombinant DNA manipulations. ..

    Article Title: Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome
    Article Snippet: E. coli NovaBlue (Novagen) strain was used as the screening host for the pETBlue-2 bearing inserts. .. The bacterial strain for the expression of the recombinant protein was E. coli BL21(DE3)pLysS (Novagen).

    Mutagenesis:

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: Paragraph title: Random mutagenesis and library screening ... The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany).

    Isolation:

    Article Title: Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides
    Article Snippet: Strains and media R. sphaeroides HJ, which was isolated in a previous study [ ], and R. sphaeroides 2.4.1 were used as a host strain and a source of genes, respectively. .. Escherichia coli NovaBlue (Novagen, Darmstadt, Germany) and S17-1 strains were used for DNA manipulation and conjugation transfer, respectively.

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany). .. Isolated colonies (960) were screened following the procedures previously described .

    Article Title: IgG Endopeptidase SeMac does not Inhibit Opsonophagocytosis of Streptococcus equi Subspecies equi by Horse Polymorphonuclear Leukocytes
    Article Snippet: The other 4 strains were isolated in 2003 from horses with strangles in Livingston (designated strain SEM1) [ ], Pony, Great Fall, and Norris in Montana. .. Escherichia coli Novablue and BL21(DE3) (Novagen, Madison, Wis.) were used for gene cloning and protein expression, respectively.

    Microscopy:

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: Cultures were examined by phase-contrast microscopy for purity and typical strain morphology before use. .. Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) and JM109 ( ) were used as hosts for cloning.

    Purification:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The PCR amplified insert was purified with SpinPrep Gel DNA Kit (Novagen, Darmstadt, Germany) and cloned via LIC into vector pET-32 Xa/LIC (EMD Millipore, Darmstadt, Germany). .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted. .. Recombinant proteins (rEch_1067 and rDPA) were purified from the soluble fraction with Ni-agarose (Sigma–Aldrich).

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: .. In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. Plasmid DNA was phenol–chloroform extracted, ethanol precipitated and resuspended in deionized and diethyl pyrocarbonate-treated water.

    Polymerase Chain Reaction:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: The PCR amplified insert was purified with SpinPrep Gel DNA Kit (Novagen, Darmstadt, Germany) and cloned via LIC into vector pET-32 Xa/LIC (EMD Millipore, Darmstadt, Germany). .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) and JM109 ( ) were used as hosts for cloning. .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen).

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: The DNA fragments encoding the full-length Ech_1067 without signal peptide (1–30 aa) and the full-length diaminopimelate aminotransferase (DPA, Ech_0886) were amplified by polymerase chain reaction (PCR) using E. chaffeensis chromosomal DNA as a template. .. Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted.

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. .. Each reaction was prepared in a reaction volume of 25 μL, using 1 μL of cDNA, set primers at a concentration of 200 nM each, and reagent SYBR Green (Power SYBR® Green PCR Master Mix; Applied Biosystems).

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. PCR products were purified with Wizard Plus SV Gel and PCR Clean-Up Systems (Promega).

    Construct:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. Positive clones were screened for via ampicillin and kanamycin resistance.

    Article Title: Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae
    Article Snippet: Strains, media, and primers Saccharomyces cerevisiae GCIΔGLR1 , GSH1 /GSH2 cocktail δ-integrated and GLR1 deleted YPH499 (ABC1193/NBRC 10505) strain was previously constructed [ ] and used for glutathione production in this study. .. Escherichia coli NovaBlue (Novagen, Darmstadt, Germany) strain was used for DNA manipulation.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) and JM109 ( ) were used as hosts for cloning. .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen).

    Plasmid Preparation:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. Positive clones were screened for via ampicillin and kanamycin resistance.

    Article Title: Treponema denticola PrcB Is Required for Expression and Activity of the PrcA-PrtP (Dentilisin) Complex ▿
    Article Snippet: Escherichia coli NovaBlue (Novagen, Inc., Madison, WI) and JM109 ( ) were used as hosts for cloning. .. Plasmid vector pSTBlue-1 (Novagen) was used for direct cloning of PCR products, and 6×His-tagged constructs were made in pET28b (Novagen).

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted. .. Escherichia coli BL21 (DE3) (Novagen) cells were transformed with the plasmid and induced to express the recombinant proteins with isopropyl-thio-ß- d -galactoside (Gold Biotechnology).

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. ..

    Article Title: A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
    Article Snippet: 2.1 Random mutagenesis and library screening A sample of the expression vector pCAL coding for the wild-type Sfβgly was submitted to random mutagenesis using the GeneMorph II kit (Agilent Technologies; Santa Clara, US) following the manufacturer's instructions. .. The random Sfβgly mutants library was used to transform E. coli NovaBlue(DE3) (Novagen, Darmstadt, Germany).

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. Plasmid DNA was phenol–chloroform extracted, ethanol precipitated and resuspended in deionized and diethyl pyrocarbonate-treated water.

    Article Title: Characterization of a Novel Serine Protease Inhibitor Gene from a Marine Metagenome
    Article Snippet: Paragraph title: 3.1. Plasmid Vectors, Bacterium Strains, and Growth Conditions ... E. coli NovaBlue (Novagen) strain was used as the screening host for the pETBlue-2 bearing inserts.

    Article Title: The Inhibitory Effect of GlmU Acetyltransferase Inhibitor TPSA on Mycobacterium tuberculosis May Be Affected Due to Its Methylation by Methyltransferase Rv0560c
    Article Snippet: Escherichia coli NovaBlue (Novagen) was used for gene cloning and BL21(DE3) (Novagen) was for GlmU protein expression. .. Cloning plasmid pJET1.2 blunt (Thermo) with the ampicillin resistance gene was used for cloning genes and re-sequencing the target genes.

    Software:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Thermal cycling conditions were: 95 °C for 3 min, followed by 32 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 1 min, and followed by a final incubation at 72 °C for 5 min. Amplification products were separated in 1.5% agarose gel in TAE buffer and stained with EthBr, then gel images were captured in a DNR Bio-Imaging System MF-ChemiBIS 2.0. (Mahale HaHamisha, Jerusalem, Israel) using Gel-Capture software. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    SYBR Green Assay:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. .. Each reaction was prepared in a reaction volume of 25 μL, using 1 μL of cDNA, set primers at a concentration of 200 nM each, and reagent SYBR Green (Power SYBR® Green PCR Master Mix; Applied Biosystems).

    Positron Emission Tomography:

    Article Title: Characterization and Recombinant Expression of Terebrid Venom Peptide from Terebra guttata
    Article Snippet: .. The pET-32 Xa/LIC:Tgu6.1 plasmid construct was transformed into E. coli NovaBlue obtained from Novagen. .. Positive clones were screened for via ampicillin and kanamycin resistance.

    Article Title: Penicillin-Binding Protein of Ehrlichia chaffeensis: Cytokine Induction Through MyD88-Dependent Pathway
    Article Snippet: The amplified fragments were digested with restriction enzymes and ligated into restriction enzyme–digested pET-33b(+) (Novagen). .. Escherichia coli Novablue (Novagen) was transformed and the plasmids were extracted.

    Agarose Gel Electrophoresis:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Thermal cycling conditions were: 95 °C for 3 min, followed by 32 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 1 min, and followed by a final incubation at 72 °C for 5 min. Amplification products were separated in 1.5% agarose gel in TAE buffer and stained with EthBr, then gel images were captured in a DNR Bio-Imaging System MF-ChemiBIS 2.0. (Mahale HaHamisha, Jerusalem, Israel) using Gel-Capture software. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    In Vitro:

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: .. In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. Plasmid DNA was phenol–chloroform extracted, ethanol precipitated and resuspended in deionized and diethyl pyrocarbonate-treated water.

    Concentration Assay:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time. .. Each reaction was prepared in a reaction volume of 25 μL, using 1 μL of cDNA, set primers at a concentration of 200 nM each, and reagent SYBR Green (Power SYBR® Green PCR Master Mix; Applied Biosystems).

    DNA Purification:

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: .. In vitro characterization of the riboswitch Plasmids were amplified in E. coli Novablue (Novagen) and purified with Wizard Plus SV Minipreps DNA Purification System (Promega). .. Plasmid DNA was phenol–chloroform extracted, ethanol precipitated and resuspended in deionized and diethyl pyrocarbonate-treated water.

    Staining:

    Article Title: Phospholipase D from Loxosceleslaeta Spider Venom Induces IL-6, IL-8, CXCL1/GRO-α, and CCL2/MCP-1 Production in Human Skin Fibroblasts and Stimulates Monocytes Migration
    Article Snippet: Thermal cycling conditions were: 95 °C for 3 min, followed by 32 cycles of 95 °C for 30 s, 58 °C for 30 s, and 72 °C for 1 min, and followed by a final incubation at 72 °C for 5 min. Amplification products were separated in 1.5% agarose gel in TAE buffer and stained with EthBr, then gel images were captured in a DNR Bio-Imaging System MF-ChemiBIS 2.0. (Mahale HaHamisha, Jerusalem, Israel) using Gel-Capture software. .. In addition, each gene was cloned into a cloning vector pCR2.1® -TOPO® using the TOPO TA Cloning® kit (Invitrogen) and transformed into E. coli Novablue (EMD Chemicals-Novagen Brand., Madison, WI, USA) Then, cloned gene plasmids were purified and used to prepare a standard curve for quantitative RT-PCR (RT-qPCR) in real time.

    Epifluorescence Microscopy:

    Article Title: S-Layer Homology Domain Proteins Csac_0678 and Csac_2722 Are Implicated in Plant Polysaccharide Deconstruction by the Extremely Thermophilic Bacterium Caldicellulosiruptor saccharolyticus
    Article Snippet: Escherichia coli NovaBlue and E. coli Rosetta(DE3) (Novagen, Madison, WI) were used as cloning and expression hosts, respectively. .. Escherichia coli NovaBlue and E. coli Rosetta(DE3) (Novagen, Madison, WI) were used as cloning and expression hosts, respectively.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Millipore novablue
    Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue/product/Millipore
    Average 98 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    novablue - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    84
    Millipore e coli novablue gigasingles
    E Coli Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue gigasingles/product/Millipore
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli novablue gigasingles - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    83
    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    83
    Millipore escherichia coli bacteria
    Escherichia Coli Bacteria, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bacteria/product/Millipore
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bacteria - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    Image Search Results