e coli novablue  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Millipore e coli novablue
    Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli <t>NovaBlue</t> plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus
    E Coli Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue/product/Millipore
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    e coli novablue - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Characterization of Fluorescent Chimeras of Cholera Toxin and Escherichia coli Heat-Labile Enterotoxins Produced by Use of the Twin Arginine Translocation System "

    Article Title: Characterization of Fluorescent Chimeras of Cholera Toxin and Escherichia coli Heat-Labile Enterotoxins Produced by Use of the Twin Arginine Translocation System

    Journal:

    doi: 10.1128/IAI.73.6.3627-3635.2005

    Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli NovaBlue plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus
    Figure Legend Snippet: Analysis of CT chimera fluorescence. (A) Expression of the GFP-CT and RFP-CT chimeras in E. coli after an overnight incubation with 0.2% l -arabinose: E. coli NovaBlue plus pTatABCE plus pJKT35 with FITC filter (frame 1) and NovaBlue plus pTatABCE plus

    Techniques Used: Fluorescence, Expressing, Incubation

    Related Articles

    Blocking Assay:

    Article Title: Collagen Induces Maturation of Human Monocyte-Derived Dendritic Cells by Signaling through Osteoclast-Associated Receptor
    Article Snippet: Most blocking experiments were performed with the use of several different high-affinity anti-OSCAR–blocking mAbs and the two above-mentioned isotype controls with identical results. .. ColIV from human placenta was purchased from Sigma-Aldrich.

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: Cells were washed and incubated with 1 % bovine serum albumine (BSA) to block unspecific binding of the antibodies. .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT.

    Article Title: Spatial and Temporal Analysis of Extracellular Matrix Proteins in the Developing Murine Heart: A Blueprint for Regeneration
    Article Snippet: .. Following a 30-min incubation at room temperature in a blocking solution, rabbit anti-mouse primary antibodies were incubated at 4°C overnight in a blocking solution containing anti-COLI (1:100, Abcam ab21286), anti-COLIV (1:50, Millipore AB748), anti-ELN (1:200, Millipore AB2039), or anti-FN (1:100, Millipore AB2033). .. SHG of whole ventricles (ventricle thickness up to ∼1.9 mm thickness; imaging depth up to ∼340 μm) was examined using multiphoton laser scanning microscopy (MPLSM).

    Incubation:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control). .. Following overnight incubation, supernatants were collected and frozen at -80°C and batch tested using MSD Human ProInflammatory 9-Plex Tissue Culture Kit K15007B-1 (measuring GM-CSF, IFN-γ, IL-1β, IL-10, IL-12p70, IL-2, IL-6, IL-8, TNF-α) run on a Meso Scale Diagnostics MESO QuickPlex SQ 120 instrument.

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: Extracellular bacteria were labelled by incubation with a goat polyclonal anti-E. coli antibody (Abcam, Cambridge, UK) diluted 1:500 in PBS with 1 % BSA for 60 min at room temperature (RT), washed and then incubated with a secondary donkey anti- goat IgG-DyLight549 conjugated antibody (Jackson ImmunoResearch Europe Ltd., Suffolk, UK) (red fluorescence) diluted 1:500 (in PBS with 1 % BSA) for 90 min at RT. .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT.

    Article Title: Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System
    Article Snippet: Scanning-Electron Microscope (SEM) Analysis Salmonella cells treated by the cell-free supernatants from the E. coli secretion system were fixed with a volume fraction of 2.5% glutaraldehyde (Sigma-Aldrich, Milwaukee, WI, USA) for 24 h at 4 °C. .. The samples were rinsed with sterile PBS buffer thrice, and then dehydrated with 30%, 50%, 70%, 80%, 90% and 100% (v /v ) graded ethanol, successively, with 15 min incubation at each concentration.

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control). .. Following overnight incubation, supernatants were collected and frozen at -80°C and batch tested using MSD Human ProInflammatory 9-Plex Tissue Culture Kit K15007B-1 (measuring GM-CSF, IFN-γ, IL-1β, IL-10, IL-12p70, IL-2, IL-6, IL-8, TNF-α) run on a Meso Scale Diagnostics MESO QuickPlex SQ 120 instrument.

    Article Title: Spatial and Temporal Analysis of Extracellular Matrix Proteins in the Developing Murine Heart: A Blueprint for Regeneration
    Article Snippet: .. Following a 30-min incubation at room temperature in a blocking solution, rabbit anti-mouse primary antibodies were incubated at 4°C overnight in a blocking solution containing anti-COLI (1:100, Abcam ab21286), anti-COLIV (1:50, Millipore AB748), anti-ELN (1:200, Millipore AB2039), or anti-FN (1:100, Millipore AB2033). .. SHG of whole ventricles (ventricle thickness up to ∼1.9 mm thickness; imaging depth up to ∼340 μm) was examined using multiphoton laser scanning microscopy (MPLSM).

    Infection:

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: Immunofluorescence of UPEC in the presence of host bladder epithelial cells Bladder 5637 cells were seeded onto well glass chamber slides (SPL Lifesciences Co., Ltd., Pocheon-city, Gyeonggi-Do, Korea), grown to subconfluency and infected with ESBL isolates as described above. .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT.

    Ex Vivo:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Paragraph title: Isolation, culture and ex vivo stimulation of Monocyte Derived Macrophages (MDMs) ... MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Derivative Assay:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Paragraph title: Isolation, culture and ex vivo stimulation of Monocyte Derived Macrophages (MDMs) ... MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Immunohistochemistry:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: .. For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used. .. Immunoreactivity was detected using fluorescence-conjugated goat anti-rabbit IgG (1:200; Chemicon).

    Article Title: Spatial and Temporal Analysis of Extracellular Matrix Proteins in the Developing Murine Heart: A Blueprint for Regeneration
    Article Snippet: Paragraph title: Fluorescence IHC ... Following a 30-min incubation at room temperature in a blocking solution, rabbit anti-mouse primary antibodies were incubated at 4°C overnight in a blocking solution containing anti-COLI (1:100, Abcam ab21286), anti-COLIV (1:50, Millipore AB748), anti-ELN (1:200, Millipore AB2039), or anti-FN (1:100, Millipore AB2033).

    Cell Culture:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: Paragraph title: Immunohistochemistry of cultured cells, harvested PDLSC sheets, and regenerated bio-root tissues ... For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used.

    Confocal Laser Scanning Microscopy:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used. .. For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used.

    Immunostaining:

    Article Title: Critical Endothelial Regulation by LRP5 during Retinal Vascular Development
    Article Snippet: .. Antibodies The following antibodies were used for immuno-staining: ColIV (EMD Millipore), CD31 (BD Pharmingen), fibronectin (Sigma), F4/80 (Invitrogen), Alexa Fluor 488 (or 555) conjugated anti-rabbit secondary antibody (Invitrogen). .. Immunofluorescence Microscopy For whole-mount retina IF staining, eyes were fixed in 4% PFA in PBS for 30min at room temperature (RT) or overnight at 4°C.

    Binding Assay:

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: Cells were washed and incubated with 1 % bovine serum albumine (BSA) to block unspecific binding of the antibodies. .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT.

    Immunofluorescence:

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: Paragraph title: Immunofluorescence of UPEC in the presence of host bladder epithelial cells ... In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT.

    Fluorescence:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used. .. Immunoreactivity was detected using fluorescence-conjugated goat anti-rabbit IgG (1:200; Chemicon).

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Upon harvesting of MDMs, viability was assessed by absence of uptake of trypan blue, and purity (ranging 96–98% MDMs) was determined by fluorescence cell sorter (FACS). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT. .. Samples were mounted with Vectashield Mounting media (Vector Laboratories Inc., Burlingame, CA, USA) containing 4′, 6-diamidino-2-phenylindole (DAPI) for staining of nuclei.

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Upon harvesting of MDMs, viability was assessed by absence of uptake of trypan blue, and purity (ranging 96–98% MDMs) was determined by fluorescence cell sorter (FACS). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Article Title: Spatial and Temporal Analysis of Extracellular Matrix Proteins in the Developing Murine Heart: A Blueprint for Regeneration
    Article Snippet: Paragraph title: Fluorescence IHC ... Following a 30-min incubation at room temperature in a blocking solution, rabbit anti-mouse primary antibodies were incubated at 4°C overnight in a blocking solution containing anti-COLI (1:100, Abcam ab21286), anti-COLIV (1:50, Millipore AB748), anti-ELN (1:200, Millipore AB2039), or anti-FN (1:100, Millipore AB2033).

    Isolation:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Paragraph title: Isolation, culture and ex vivo stimulation of Monocyte Derived Macrophages (MDMs) ... MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Negative Control:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control). .. Following overnight incubation, supernatants were collected and frozen at -80°C and batch tested using MSD Human ProInflammatory 9-Plex Tissue Culture Kit K15007B-1 (measuring GM-CSF, IFN-γ, IL-1β, IL-10, IL-12p70, IL-2, IL-6, IL-8, TNF-α) run on a Meso Scale Diagnostics MESO QuickPlex SQ 120 instrument.

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control). .. Following overnight incubation, supernatants were collected and frozen at -80°C and batch tested using MSD Human ProInflammatory 9-Plex Tissue Culture Kit K15007B-1 (measuring GM-CSF, IFN-γ, IL-1β, IL-10, IL-12p70, IL-2, IL-6, IL-8, TNF-α) run on a Meso Scale Diagnostics MESO QuickPlex SQ 120 instrument.

    Microscopy:

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT. .. Images were obtained and processed using an Olympus BX60 fluorescence microscope equipped with an Olympus DP71 camera and Adobe Photoshop software.

    Article Title: Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System
    Article Snippet: .. Scanning-Electron Microscope (SEM) Analysis Salmonella cells treated by the cell-free supernatants from the E. coli secretion system were fixed with a volume fraction of 2.5% glutaraldehyde (Sigma-Aldrich, Milwaukee, WI, USA) for 24 h at 4 °C. .. The samples were rinsed with sterile PBS buffer thrice, and then dehydrated with 30%, 50%, 70%, 80%, 90% and 100% (v /v ) graded ethanol, successively, with 15 min incubation at each concentration.

    Purification:

    Article Title: Collagen Induces Maturation of Human Monocyte-Derived Dendritic Cells by Signaling through Osteoclast-Associated Receptor
    Article Snippet: ColIII purified from human placenta was purchased from Millipore. .. ColIV from human placenta was purchased from Sigma-Aldrich.

    Labeling:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Monocytes were prepared from freshly drawn blood samples through negative selection using the Miltenyi Biotec Monocyte Isolation Kit II, which provides an indirect magnetic labeling approach for the isolation of untouched monocytes from human peripheral blood mononuclear cells (PBMCs). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Monocytes were prepared from freshly drawn blood samples through negative selection using the Miltenyi Biotec Monocyte Isolation Kit II, which provides an indirect magnetic labeling approach for the isolation of untouched monocytes from human peripheral blood mononuclear cells (PBMCs). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    FACS:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Upon harvesting of MDMs, viability was assessed by absence of uptake of trypan blue, and purity (ranging 96–98% MDMs) was determined by fluorescence cell sorter (FACS). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Upon harvesting of MDMs, viability was assessed by absence of uptake of trypan blue, and purity (ranging 96–98% MDMs) was determined by fluorescence cell sorter (FACS). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Plasmid Preparation:

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT. .. Samples were mounted with Vectashield Mounting media (Vector Laboratories Inc., Burlingame, CA, USA) containing 4′, 6-diamidino-2-phenylindole (DAPI) for staining of nuclei.

    Software:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used. .. The sections were observed using an Axio Imager A1 (Carl Zeiss) with an AxioCAM MRc5 (Zeiss) and processed with AxioVision software (Zeiss).

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT. .. Images were obtained and processed using an Olympus BX60 fluorescence microscope equipped with an Olympus DP71 camera and Adobe Photoshop software.

    Functional Assay:

    Article Title: Collagen Induces Maturation of Human Monocyte-Derived Dendritic Cells by Signaling through Osteoclast-Associated Receptor
    Article Snippet: Paragraph title: Proteins and Abs for functional assays ... ColIV from human placenta was purchased from Sigma-Aldrich.

    Selection:

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Monocytes were prepared from freshly drawn blood samples through negative selection using the Miltenyi Biotec Monocyte Isolation Kit II, which provides an indirect magnetic labeling approach for the isolation of untouched monocytes from human peripheral blood mononuclear cells (PBMCs). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Article Title: Interplay of strain and race/ethnicity in the innate immune response to M. tuberculosis
    Article Snippet: Monocytes were prepared from freshly drawn blood samples through negative selection using the Miltenyi Biotec Monocyte Isolation Kit II, which provides an indirect magnetic labeling approach for the isolation of untouched monocytes from human peripheral blood mononuclear cells (PBMCs). .. MDMs from each study participant, were incubated at 1x105 for 18 hours with 10 ug / ml whole cell lysate of M . tuberculosis strains, and to agonists of TLR2 (10 ug / ml Lipoteichoic acid (LTA) from S. aureus, Sigma, L 2515) and TLR4 (50 ng/ml lipopolysaccharide (LPS) from E. coli Sigma, L-2880); and culture medium (negative control).

    Concentration Assay:

    Article Title: Anti-Salmonella Activity Modulation of Mastoparan V1—A Wasp Venom Toxin—Using Protease Inhibitors, and Its Efficient Production via an Escherichia coli Secretion System
    Article Snippet: Scanning-Electron Microscope (SEM) Analysis Salmonella cells treated by the cell-free supernatants from the E. coli secretion system were fixed with a volume fraction of 2.5% glutaraldehyde (Sigma-Aldrich, Milwaukee, WI, USA) for 24 h at 4 °C. .. The samples were rinsed with sterile PBS buffer thrice, and then dehydrated with 30%, 50%, 70%, 80%, 90% and 100% (v /v ) graded ethanol, successively, with 15 min incubation at each concentration.

    Staining:

    Article Title: Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine
    Article Snippet: For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used. .. For immunohistochemistry, the primary antibodies anti-dentin sialoprotein (1:200; LF-151), anti-COLI (1:1,000; Sigma-Aldrich), and anti-von Willibrand factor (αvWF pAb, 1:100; Chemicon) were used.

    Article Title: Carbon monoxide releasing molecule-2 (CORM-2) inhibits growth of multidrug-resistant uropathogenic Escherichia coli in biofilm and following host cell colonization
    Article Snippet: .. In order to label intracellular bacteria the host cells were permeabilized with 0.5 % Triton X-100 for 10 min, washed and reprobed with the goat anti-E. coli antibody for 60 min. After wash, the bacteria were stained with a secondary rabbit anti-goat IgG-FITC conjugated antibody (Sigma-Aldrich) (green fluorescence) diluted 1:400 (in PBS with 1 % BSA) for 100 min at RT. .. Samples were mounted with Vectashield Mounting media (Vector Laboratories Inc., Burlingame, CA, USA) containing 4′, 6-diamidino-2-phenylindole (DAPI) for staining of nuclei.

    other:

    Article Title: Activation of Arterial Program Drives Development of Definitive Hemogenic Endothelium with Lymphoid Potential
    Article Snippet: Singular iETS1 hESCs were plated 10,000 cells/cm2 to 6 well plates coated with ColIV (Sigma).

    Article Title: Tenascin C Promotes Hematoendothelial Development and T Lymphoid Commitment from Human Pluripotent Stem Cells in Chemically Defined Conditions
    Article Snippet: Single cells were plated at an optimized density ranging from 5,000 cells/cm2 to 15,000 cells/cm2 (depending on the cell line) onto six-well plates coated with 0.5 μg/cm2 of ColIV (Sigma-Aldrich) or 0.5 μg/cm2 TenC (Millipore) in E8 medium supplemented with 10 μM Rho kinase inhibitor (Tocris Y-27632).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore escherichia coli strain novablue
    Escherichia Coli Strain Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain novablue/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain novablue - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Millipore novablue
    Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue/product/Millipore
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    novablue - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    93
    Millipore e coli novablue competent cells
    E Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue competent cells/product/Millipore
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    e coli novablue competent cells - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results