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Merck KGaA e coli novablue
E Coli Novablue, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli novablue/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
e coli novablue - by Bioz Stars, 2019-12
86/100 stars

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Clone Assay:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The signal peptide cleavage sites of the open reading frames (ORFs) were predicted using the SignalP server ( ). .. The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: These were complementary to the most conserved regions displayed in multiple sequence alignments produced with MAFFT vs. 6 , of 7 reference Culex flavivirus sequences deposited in the GenBank public database under accession numbers AB377213 (strain NIID-21-2), EU879060 (strain CxFV-Mex07), FJ502995 (strain HOU24518), FJ644291 (strain VN180), FJ663034 (strain Iowa07), GQ165808 (strain Uganda08), NC_008604 (strain Tokyo). .. DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing. .. Partial mitochondrial cytochrome c oxidase subunit I (COI) sequences were amplified from total genomic DNA, extracted from mosquito homogenates with the ZymoBead™ Genomic DNA kit (Zymo Research, Irvine, CA), and the PuRe Taq Ready-to-Go PCR Beads (GE Healthcare, Dornstadt, Germany), using primers and reaction conditions previously described ( ; described by ).

Positron Emission Tomography:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The signal peptide cleavage sites of the open reading frames (ORFs) were predicted using the SignalP server ( ). .. The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

Infection:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: Total RNA was also extracted from C6/36 infected and non-infected cells using the INSTANT Virus RNA kit (Analytik Jena AG, Jena, Germany), also following the supplier's instructions. .. DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing.

Purification:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: These were complementary to the most conserved regions displayed in multiple sequence alignments produced with MAFFT vs. 6 , of 7 reference Culex flavivirus sequences deposited in the GenBank public database under accession numbers AB377213 (strain NIID-21-2), EU879060 (strain CxFV-Mex07), FJ502995 (strain HOU24518), FJ644291 (strain VN180), FJ663034 (strain Iowa07), GQ165808 (strain Uganda08), NC_008604 (strain Tokyo). .. DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing. .. Partial mitochondrial cytochrome c oxidase subunit I (COI) sequences were amplified from total genomic DNA, extracted from mosquito homogenates with the ZymoBead™ Genomic DNA kit (Zymo Research, Irvine, CA), and the PuRe Taq Ready-to-Go PCR Beads (GE Healthcare, Dornstadt, Germany), using primers and reaction conditions previously described ( ; described by ).

Produced:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: These were complementary to the most conserved regions displayed in multiple sequence alignments produced with MAFFT vs. 6 , of 7 reference Culex flavivirus sequences deposited in the GenBank public database under accession numbers AB377213 (strain NIID-21-2), EU879060 (strain CxFV-Mex07), FJ502995 (strain HOU24518), FJ644291 (strain VN180), FJ663034 (strain Iowa07), GQ165808 (strain Uganda08), NC_008604 (strain Tokyo). .. DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing.

Polymerase Chain Reaction:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The signal peptide cleavage sites of the open reading frames (ORFs) were predicted using the SignalP server ( ). .. The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

Amplification:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: Paragraph title: Nucleotide sequence amplification and DNA sequencing ... DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing.

DNA Sequencing:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: These were complementary to the most conserved regions displayed in multiple sequence alignments produced with MAFFT vs. 6 , of 7 reference Culex flavivirus sequences deposited in the GenBank public database under accession numbers AB377213 (strain NIID-21-2), EU879060 (strain CxFV-Mex07), FJ502995 (strain HOU24518), FJ644291 (strain VN180), FJ663034 (strain Iowa07), GQ165808 (strain Uganda08), NC_008604 (strain Tokyo). .. DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing. .. Partial mitochondrial cytochrome c oxidase subunit I (COI) sequences were amplified from total genomic DNA, extracted from mosquito homogenates with the ZymoBead™ Genomic DNA kit (Zymo Research, Irvine, CA), and the PuRe Taq Ready-to-Go PCR Beads (GE Healthcare, Dornstadt, Germany), using primers and reaction conditions previously described ( ; described by ).

Expressing:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: Paragraph title: Cloning and expression of candidate vaccine proteins. ... The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions.

Sequencing:

Article Title: Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
Article Snippet: Paragraph title: Nucleotide sequence amplification and DNA sequencing ... DNA amplicons were purified with the DNA Clean & Concentrator™-5 (Zymo Research, Irvine, CA) and either directly sequenced or cloned in either pGEM® -T Easy (Promega, Madison, WI) or CloneJET™ (Fermentas, Vilnius, Lithuania) using Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) as host, prior to DNA sequencing.

Transformation Assay:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The signal peptide cleavage sites of the open reading frames (ORFs) were predicted using the SignalP server ( ). .. The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

Article Title: Development and Application of a Method for Counterselectable In-Frame Deletion in Clostridium perfringens
Article Snippet: Comparison of the two disruptants in terms of growth ability and the background levels of secreted proteins showed that HN1314 is more useful than ΗΝ1303 as a host for the large-scale production of recombinant proteins. .. Escherichia coli NovaBlue (Merck KGaA, Darmstadt, Germany) was used as the recipient strain for transformation and was grown in LB medium at 37°C. .. Clostridium acetobutylicum ATCC 824 was obtained from the Riken BioResource Center and was used as the source of the galK gene in the suicide vector.

Recombinant:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

SDS Page:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

Plasmid Preparation:

Article Title: Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs
Article Snippet: The signal peptide cleavage sites of the open reading frames (ORFs) were predicted using the SignalP server ( ). .. The PCR products of candidate genes were cloned into the pET-30 Ek/LIC vector (Merck Millipore), and fusion plasmids were transformed into E. coli NovaBlue (Merck Millipore) according to the manufacturer's instructions. .. The positive recombinants were confirmed by PCR and DNA sequencing and then transformed into E. coli BL21(DE3) (Merck Millipore) for expression.

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    Merck KGaA e coli novablue
    E Coli Novablue, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue/product/Merck KGaA
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli novablue - by Bioz Stars, 2019-12
    86/100 stars
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