e coli novablue single competent cells  (Millipore)


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    Structured Review

    Millipore e coli novablue single competent cells
    E Coli Novablue Single Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue single competent cells/product/Millipore
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli novablue single competent cells - by Bioz Stars, 2020-04
    82/100 stars

    Related Products / Commonly Used Together

    amplicon
    pt7blue-2 lic vector

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    Related Articles

    Amplification:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated. .. To identify colonies harboring the correct recombinant plasmid, colonies were selected, placed in 100 μl of H2 O (after generating a master plate with all analyzed colonies), and boiled for 10 min, and then 1 μl was used as template in PCR with the E46(+)-E470(−) primer set.

    Infection:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: Paragraph title: Immunoblot procedures, ligase-independent cloning (LIC), and expression of OspE paralogs: analysis of the humoral immune response to OspE in experimentally infected mice. ... The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Mouse Assay:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: Paragraph title: Immunoblot procedures, ligase-independent cloning (LIC), and expression of OspE paralogs: analysis of the humoral immune response to OspE in experimentally infected mice. ... The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Generated:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: After PCR was performed, the single-stranded overhangs on the amplicon were generated by treatment with T4 DNA polymerase in the presence of dATP (other deoxynucleoside triphosphates are omitted) as described by the manufacturer (Novagen). .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Activity Assay:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: When these bases are encountered by the DNA polymerase, the 5′-3′ polymerase activity counteracts its exonuclease activity, resulting in an amplicon with single-stranded overhangs that complement the vector. .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Expressing:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: Paragraph title: Immunoblot procedures, ligase-independent cloning (LIC), and expression of OspE paralogs: analysis of the humoral immune response to OspE in experimentally infected mice. ... The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Ligase Independent Cloning:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: Paragraph title: Immunoblot procedures, ligase-independent cloning (LIC), and expression of OspE paralogs: analysis of the humoral immune response to OspE in experimentally infected mice. ... The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Polymerase Chain Reaction:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: After PCR was performed, the single-stranded overhangs on the amplicon were generated by treatment with T4 DNA polymerase in the presence of dATP (other deoxynucleoside triphosphates are omitted) as described by the manufacturer (Novagen). .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated.

    Transformation Assay:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated. .. To identify colonies harboring the correct recombinant plasmid, colonies were selected, placed in 100 μl of H2 O (after generating a master plate with all analyzed colonies), and boiled for 10 min, and then 1 μl was used as template in PCR with the E46(+)-E470(−) primer set.

    Recombinant:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated. .. To identify colonies harboring the correct recombinant plasmid, colonies were selected, placed in 100 μl of H2 O (after generating a master plate with all analyzed colonies), and boiled for 10 min, and then 1 μl was used as template in PCR with the E46(+)-E470(−) primer set.

    Plasmid Preparation:

    Article Title: Mutation and Recombination in the Upstream Homology Box-Flanked ospE-Related Genes of the Lyme Disease Spirochetes Result in the Development of New Antigenic Variants during Infection
    Article Snippet: .. The treated amplicon and pT7Blue-2 LIC vector were then annealed and transformed into E. coli NovaBlue single competent cells (Novagen), using a standard transformation protocol, where covalent bond formation occurs and the plasmid is propagated. .. To identify colonies harboring the correct recombinant plasmid, colonies were selected, placed in 100 μl of H2 O (after generating a master plate with all analyzed colonies), and boiled for 10 min, and then 1 μl was used as template in PCR with the E46(+)-E470(−) primer set.

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  • 92
    Millipore e coli novablue singles competent cells
    E Coli Novablue Singles Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue singles competent cells/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli novablue singles competent cells - by Bioz Stars, 2020-04
    92/100 stars
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