e coli novablue gigasingles  (Millipore)


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    Structured Review

    Millipore e coli novablue gigasingles
    E Coli Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue gigasingles/product/Millipore
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli novablue gigasingles - by Bioz Stars, 2020-01
    84/100 stars

    Related Products / Commonly Used Together

    pcr
    dpni
    dpni restriction endonuclease

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    Related Articles

    Clone Assay:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Positron Emission Tomography:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: VapC and PIN domain encoding genes were cloned into pRSF or pET46 Ek/LIC vector using the pET-46 Ek/LIC Vector Kit (Novagen). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Mutagenesis:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Isolation:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Introduce:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Purification:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Polymerase Chain Reaction:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Generated:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Cell Culture:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Recovered cells were cultured overnight on LB plates with 1.5% (w/w) agar supplemented with either ampicillin/carbenicillin or kanamycin.

    Expressing:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The expression plasmid for C84A Tk-KPR inserted into pET-21a had previously been constructed (Aikawa et al. , 2016 ).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Sequencing:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Plasmid miniprep was done using QIAprep Spin Miniprep Kit (Qiagen) and the presence/sequence of the insert was confirmed by sequencing (Eton Bioscience Inc. (Durham NC) or Genewiz Inc. (Research Triangle Park NC)).

    Construct:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The expression plasmid for C84A Tk-KPR inserted into pET-21a had previously been constructed (Aikawa et al. , 2016 ).

    Transformation Assay:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Recovered cells were cultured overnight on LB plates with 1.5% (w/w) agar supplemented with either ampicillin/carbenicillin or kanamycin.

    Plasmid Preparation:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Paragraph title: 2.1. Plasmid construction   ... The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: In either case, a stop codon was included to prevent inclusion of a C-terminal vector-encoded S-Tag in the final protein product. .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

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  • 84
    Millipore e coli novablue gigasingles
    E Coli Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue gigasingles/product/Millipore
    Average 84 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli novablue gigasingles - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    78
    Millipore e coli strain novablue gigasingles
    E Coli Strain Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain novablue gigasingles/product/Millipore
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli strain novablue gigasingles - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

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