e coli novablue gigasingles  (Millipore)


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    Structured Review

    Millipore e coli novablue gigasingles
    E Coli Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue gigasingles/product/Millipore
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli novablue gigasingles - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

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    dpni
    dpni restriction endonuclease

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    Related Articles

    Clone Assay:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: .. Escherichia coli NovaBlue GigaSingles™ (EMD Millipore) competent cells were used as the cloning host and E. coli Rosetta™ 2(DE3) Singles™ (EMD Millipore) was used as the expression host. .. The E. coli strains were cultivated in Luria-Bertani (LB) media (10 g/L casein peptone, 5 g/L yeast extract, 10 g/L sodium chloride) in the presence of either kanamycin (50 μg/ mL) or ampicillin/carbenicillin (50 μg/mL) for pRSF and pET46 Ek/LIC cloning vectors (Novagen, Madison, WI), respectively.

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells. .. Plasmid DNA was isolated from several clones using a Qiagen kit; mutagenesis results were verified by sequencing.

    Positron Emission Tomography:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: VapC and PIN domain encoding genes were cloned into pRSF or pET46 Ek/LIC vector using the pET-46 Ek/LIC Vector Kit (Novagen). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Mutagenesis:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: Paragraph title: Site-directed mutagenesis ... The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells.

    Isolation:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells. .. Plasmid DNA was isolated from several clones using a Qiagen kit; mutagenesis results were verified by sequencing.

    Introduce:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Site-directed mutagenesis was performed to introduce Y60A or W129A mutations into wild-type (WT) Tk-KPR inserted into pET-21a (Tomita et al. , 2013 ). .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Purification:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Polymerase Chain Reaction:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: After one primer extension cycle, two reaction mixtures were pooled together and standard PCR was performed (25 cycles). .. The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells.

    Generated:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Genomic DNA was isolated from Metallosphaera species by methods described previously , and PCR products were generated by following established molecular biology techniques ( ). .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Cell Culture:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Recovered cells were cultured overnight on LB plates with 1.5% (w/w) agar supplemented with either ampicillin/carbenicillin or kanamycin.

    Expressing:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: .. Escherichia coli NovaBlue GigaSingles™ (EMD Millipore) competent cells were used as the cloning host and E. coli Rosetta™ 2(DE3) Singles™ (EMD Millipore) was used as the expression host. .. The E. coli strains were cultivated in Luria-Bertani (LB) media (10 g/L casein peptone, 5 g/L yeast extract, 10 g/L sodium chloride) in the presence of either kanamycin (50 μg/ mL) or ampicillin/carbenicillin (50 μg/mL) for pRSF and pET46 Ek/LIC cloning vectors (Novagen, Madison, WI), respectively.

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The expression plasmid for C84A Tk-KPR inserted into pET-21a had previously been constructed (Aikawa et al. , 2016 ).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Paragraph title: Cloning, expression and purification of VapCs, PIN domain proteins and VapBs ... Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Sequencing:

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Plasmid miniprep was done using QIAprep Spin Miniprep Kit (Qiagen) and the presence/sequence of the insert was confirmed by sequencing (Eton Bioscience Inc. (Durham NC) or Genewiz Inc. (Research Triangle Park NC)).

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells. .. Plasmid DNA was isolated from several clones using a Qiagen kit; mutagenesis results were verified by sequencing.

    Construct:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The expression plasmid for C84A Tk-KPR inserted into pET-21a had previously been constructed (Aikawa et al. , 2016 ).

    Transformation Assay:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: .. The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany). .. The plasmids were purified using a QIAprep Spin Miniprep Kit (Qiagen, Hilden, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock. .. Recovered cells were cultured overnight on LB plates with 1.5% (w/w) agar supplemented with either ampicillin/carbenicillin or kanamycin.

    Plasmid Preparation:

    Article Title: Crystal structure of ketopantoate reductase from Thermococcus kodakarensis complexed with NADP+
    Article Snippet: Paragraph title: 2.1. Plasmid construction   ... The PCR products were treated with DpnI and were transformed into E. coli NovaBlue GigaSingles (Novagen, Darmstadt, Germany).

    Article Title: VapC Toxins Drive Cellular Dormancy under Uranium Stress for the Extreme Thermoacidophile Metallosphaera prunae
    Article Snippet: In either case, a stop codon was included to prevent inclusion of a C-terminal vector-encoded S-Tag in the final protein product. .. Assembled vectors were transformed into E. coli NovaBlue GigaSingles™ (EMD Millipore) competent cells by heat shock.

    Article Title: A Truncation of the Disordered Loop Located within the C-Terminal Domain of the Transcriptional Regulator HlyIIR Remodels Its Structure
    Article Snippet: The resulting mixture was treated with DpnI restriction endonuclease and used to transform E. coli NovaBlue GigaSingles (Novagen) competent cells. .. Plasmid DNA was isolated from several clones using a Qiagen kit; mutagenesis results were verified by sequencing.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Millipore e coli novablue gigasingles
    E Coli Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue gigasingles/product/Millipore
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli novablue gigasingles - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    86
    Millipore e coli strain novablue gigasingles
    E Coli Strain Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain novablue gigasingles/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli strain novablue gigasingles - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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