e coli novablue de3 bacteria  (Millipore)


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    Structured Review

    Millipore e coli novablue de3 bacteria
    E Coli Novablue De3 Bacteria, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue de3 bacteria/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli novablue de3 bacteria - by Bioz Stars, 2020-03
    80/100 stars

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    troponin t

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    Mutagenesis:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The primers used in the PCR were the 5′ primer (identical for both reactions), 5′-AGAGAGCATATGCTGACATCGAAGAGGTGGTGG-3′, and the 3′ primer for the human cardiac TnT, 5′-AGAGAGGATCCTATTTCCAGCGACCGGTGACT-3′; for the generation of the Δ14TnT mutant the 3′ primer was 5′-GAGAGGATCCTAGACTTTCTGGTTATCGTTGATCC-3′. .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    Subcloning:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: We received the clone for human cardiac TnT2 from Dr. Samson and used it as a template in a polymerase chain reaction (PCR) to produce hc TnT2 cDNA suitable for subcloning in a pSBETA expression vector and to produce cDNA coding for the truncated Δ14 TnT mutant. .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    Purification:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ). .. The bacterial pellet was sonicated in 6 M urea, 10 mM sodium citrate, pH 7.0, 2 mM EDTA, and 1 mM dithiothreitol.

    Sequencing:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The DNA sequence was confirmed using the cyclist EXO-pfu DNA sequencing kit (Stratagene) and additionally by cycle sequencing with the ABI Prism big dye terminator cycle sequencing ready reaction kit and resolving the products on an ABI Prism 377 automated sequencer (Perkin-Elmer/Applied Biosystems). .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    DNA Sequencing:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The DNA sequence was confirmed using the cyclist EXO-pfu DNA sequencing kit (Stratagene) and additionally by cycle sequencing with the ABI Prism big dye terminator cycle sequencing ready reaction kit and resolving the products on an ABI Prism 377 automated sequencer (Perkin-Elmer/Applied Biosystems). .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    Expressing:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The Nde I/ Bam HI restriction enzyme-digested PCR products were purified and inserted at the same restriction sites of the pSBETA expression vector (from Dr. H.-H. Steinbiss). .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    Polymerase Chain Reaction:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The Nde I/ Bam HI restriction enzyme-digested PCR products were purified and inserted at the same restriction sites of the pSBETA expression vector (from Dr. H.-H. Steinbiss). .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

    Sonication:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ). .. The bacterial pellet was sonicated in 6 M urea, 10 mM sodium citrate, pH 7.0, 2 mM EDTA, and 1 mM dithiothreitol.

    Transformation Assay:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ). .. The bacterial pellet was sonicated in 6 M urea, 10 mM sodium citrate, pH 7.0, 2 mM EDTA, and 1 mM dithiothreitol.

    Plasmid Preparation:

    Article Title: The ?14 Mutation of Human Cardiac Troponin T Enhances ATPase Activity and Alters the Cooperative Binding of S1-ADP to Regulated Actin †
    Article Snippet: The Nde I/ Bam HI restriction enzyme-digested PCR products were purified and inserted at the same restriction sites of the pSBETA expression vector (from Dr. H.-H. Steinbiss). .. Troponin T was expressed in freshly transformed E. coli NovaBlue (DE3) bacteria (Novagen) and purified by modifications of published methods ( ).

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    Millipore escherichia coli de3 novablue cells
    Escherichia Coli De3 Novablue Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli de3 novablue cells/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli de3 novablue cells - by Bioz Stars, 2020-03
    85/100 stars
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