e coli novablue competent cells  (Millipore)


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    Structured Review

    Millipore e coli novablue competent cells
    E Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue competent cells/product/Millipore
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    e coli novablue competent cells - by Bioz Stars, 2020-05
    86/100 stars

    Related Products / Commonly Used Together

    pcr
    single-stranded overhang regions
    puc19
    templiphi

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    Related Articles

    Clone Assay:

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: Reconstruction of metabolic pathway for isobutanol production in Escherichia coli
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan), and the primer pairs are listed in Additional file : Table S1.

    Article Title: Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction was performed using KOD FX Neo (TOYOBO, Osaka, Japan).

    Article Title: Engineering a synthetic pathway for maleate in Escherichia coli
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen, Cambridge, MA, USA) were used for gene cloning. .. Polymerase chain reaction (PCR) was performed using the KOD FX Neo DNA polymerase (Toyobo, Osaka, Japan) and the appropriate primer pairs.

    Amplification:

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Incubation:

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Expressing:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Polymerase Chain Reaction:

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

    Transformation Assay:

    Article Title: Crystallographic insights into the structure–activity relationships of diazaborine enoyl-ACP reductase inhibitors
    Article Snippet: .. The annealed products were transformed into E. coli NovaBlue competent cells (Novagen Technical Bulletin No. 163) and confirmed. .. The recombinant plasmid (pET30-ecFabI) was isolated and retransformed into the expression host E. coli BL21 (DE3) (Supplementary Table S1).

    Article Title: Primate evolution of the recombination regulator PRDM9
    Article Snippet: .. Size-selected products were cloned into pUC19 (InFusion, Invitrogen), transformed into E. coli NovaBlue competent cells (EMD Millipore), and 4–24 single colonies for each PCR product were picked by hand for amplification by TempliPhi (GE Healthcare Life Sciences). .. Selecting multiple clones minimized the false-positive rate of any rare chimeric product that had the same size as a true allele.

    Plasmid Preparation:

    Article Title: Cold-Adapted ?-Galactosidase from the Antarctic Psychrophile Pseudoalteromonas haloplanktis
    Article Snippet: .. E. coli NovaBlue competent cells (Novagen) carrying the expression vector pET22b-β-galactosidase were grown at 18°C in L broth containing 100 mg of ampicillin liter. .. When A 595 reached 0.6, expression of the enzyme was induced by IPTG to a final concentration of 1 mM, and the cells were further cultivated at 18°C for 20 h. The cells were then harvested by centrifugation and resuspended in buffer A.

    Article Title: The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 ? resolution
    Article Snippet: .. The vector and PCR products were incubated together for 20 min to allow annealing of the single-stranded overhang regions (Aslanidis & de Jong, 1990 ) and the mixture was directly added to E. coli Novablue competent cells (Novagen), which were plated and grown overnight on antibiotic-containing media. .. Kanamycin-resistant colonies were picked the following day and used to inoculate overnight cultures, from which recombinant plasmids were purified.

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  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 86
    Millipore e coli novablue competent cells
    E Coli Novablue Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue competent cells/product/Millipore
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli novablue competent cells - by Bioz Stars, 2020-05
    86/100 stars
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