e coli novablue λde3  (Millipore)


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    Structured Review

    Millipore e coli novablue λde3
    E Coli Novablue λde3, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli novablue λde3/product/Millipore
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli novablue λde3 - by Bioz Stars, 2020-03
    79/100 stars

    Related Products / Commonly Used Together

    hrcc
    hrcqb

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    Related Articles

    Concentration Assay:

    Article Title: Negative Regulation of hrp Genes in Pseudomonas syringae by HrpV
    Article Snippet: HrcC and HrcQB were purified from E. coli NovaBlue (λDE3) (Novagen, Madison, Wis.) carrying pNCHU316 and pNCHU366, respectively, according to previously described procedures ( ). .. Two hundred microliters of the corresponding antiserum and sodium azide at a final concentration of 0.02% were added into the cell lysate and incubated at room temperature for 2 h. The mixtures were centrifuged at 20,000 × g for 30 min, and the preabsorbed antisera were collected from the supernatant.

    Incubation:

    Article Title: Negative Regulation of hrp Genes in Pseudomonas syringae by HrpV
    Article Snippet: HrcC and HrcQB were purified from E. coli NovaBlue (λDE3) (Novagen, Madison, Wis.) carrying pNCHU316 and pNCHU366, respectively, according to previously described procedures ( ). .. Five-milliliter cultures of E. coli and P. syringae pv. syringae 61 mutants ( P. syringae pv. syringae 61–N393 and P. syringae pv. syringae 61–N322 for incubation with HrcC and HrcQB antibodies, respectively) were grown overnight to stationary phase, washed several times with the same volume of 1× phosphate-buffered saline (0.058 M Na2 HPO4 , 0.017 M NaH2 PO4 · H2 O, 0.068 M NaCl), sonicated, incubated at 100°C for 10 min, and then allowed to cool to room temperature.

    Purification:

    Article Title: Negative Regulation of hrp Genes in Pseudomonas syringae by HrpV
    Article Snippet: .. HrcC and HrcQB were purified from E. coli NovaBlue (λDE3) (Novagen, Madison, Wis.) carrying pNCHU316 and pNCHU366, respectively, according to previously described procedures ( ). ..

    Sonication:

    Article Title: Negative Regulation of hrp Genes in Pseudomonas syringae by HrpV
    Article Snippet: HrcC and HrcQB were purified from E. coli NovaBlue (λDE3) (Novagen, Madison, Wis.) carrying pNCHU316 and pNCHU366, respectively, according to previously described procedures ( ). .. Five-milliliter cultures of E. coli and P. syringae pv. syringae 61 mutants ( P. syringae pv. syringae 61–N393 and P. syringae pv. syringae 61–N322 for incubation with HrcC and HrcQB antibodies, respectively) were grown overnight to stationary phase, washed several times with the same volume of 1× phosphate-buffered saline (0.058 M Na2 HPO4 , 0.017 M NaH2 PO4 · H2 O, 0.068 M NaCl), sonicated, incubated at 100°C for 10 min, and then allowed to cool to room temperature.