e coli negative selection screen novablue  (Millipore)


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    Structured Review

    Millipore e coli negative selection screen novablue
    E Coli Negative Selection Screen Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli negative selection screen novablue/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli negative selection screen novablue - by Bioz Stars, 2020-03
    86/100 stars

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    Related Articles

    Negative Control:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. A negative control was obtained by transforming the E. coli with pUC19, which lacks the target site.

    Selection:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: .. E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. Surviving colonies were scraped and cultured in ZymoBroth with 25 μg/mL chloramphenicol to an O.D. of 0.4–0.6 and made competent using a Mix & Go kit (Zymo).

    Mutagenesis:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. For each mutant PAM screened, the competent E. coli pool was transformed with 100 ng target plasmid containing the mutant PAM, incubated on ice for 15–30 min, heat shocked at 42 °C for 30s, and plated on LB agar (Affymetrix) containing 100 μg/mL ampicillin and 25 μg/mL chloramphenicol in the absence of IPTG.

    Cell Culture:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. Surviving colonies were scraped and cultured in ZymoBroth with 25 μg/mL chloramphenicol to an O.D. of 0.4–0.6 and made competent using a Mix & Go kit (Zymo).

    Incubation:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. For each mutant PAM screened, the competent E. coli pool was transformed with 100 ng target plasmid containing the mutant PAM, incubated on ice for 15–30 min, heat shocked at 42 °C for 30s, and plated on LB agar (Affymetrix) containing 100 μg/mL ampicillin and 25 μg/mL chloramphenicol in the absence of IPTG.

    Transformation Assay:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: .. E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. Surviving colonies were scraped and cultured in ZymoBroth with 25 μg/mL chloramphenicol to an O.D. of 0.4–0.6 and made competent using a Mix & Go kit (Zymo).

    Variant Assay:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: .. E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. Surviving colonies were scraped and cultured in ZymoBroth with 25 μg/mL chloramphenicol to an O.D. of 0.4–0.6 and made competent using a Mix & Go kit (Zymo).

    Plasmid Preparation:

    Article Title: Engineered Cpf1 variants with altered PAM specificities increase genome targeting range
    Article Snippet: E. coli negative selection screen NovaBlue(DE3) E. coli (Novagen) were transformed with the variant library and plated on LB agar containing 25 μg/mL chloramphenicol. .. For each mutant PAM screened, the competent E. coli pool was transformed with 100 ng target plasmid containing the mutant PAM, incubated on ice for 15–30 min, heat shocked at 42 °C for 30s, and plated on LB agar (Affymetrix) containing 100 μg/mL ampicillin and 25 μg/mL chloramphenicol in the absence of IPTG.

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