e coli dna polymerase i  (Thermo Fisher)


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    Thermo Fisher e coli dna polymerase i
    E Coli Dna Polymerase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dna polymerase i/product/Thermo Fisher
    Average 99 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    e coli dna polymerase i - by Bioz Stars, 2020-02
    99/100 stars

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    Amplification:

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: Furthermore, the primers were chosen such that the amplicon includes an intronic sequence, which permits the identification of genomic DNA contamination. .. Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis.

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Paragraph title: Unbiased amplification of TCR and BCR genes and sequencing ... After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Synthesized:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: .. The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA). .. Double-stranded cDNA was purified with a Qiaquick PCR purification kit (Qiagen), and sheared with a nebulizer (Invitrogen, CA, USA) into 200–250 bp fragments.

    Article Title: Genome Organization Drives Chromosome Fragility
    Article Snippet: .. First-strand cDNA synthesis of the captured nascent RNA was done using SuperScript VILO cDNA synthesis kit (Invitrogen), following AMPure XP purification (1.8X) and elution in 20 µl second-strand cDNA was synthesized with 0.6 mM dNTPs in the presence of 2 units of RNase H (Invitrogen) and 20 units of E. coli DNA polymerase I (Invitrogen) in a total volume of 30 µl for 2.5 hours at 16°C. ..

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: .. Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis. ..

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen). .. The ds-cDNA was blunted with T4 DNA polymerase (Invitrogen).

    Article Title: hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay †
    Article Snippet: .. The first strand was synthesized at 42°C for 1 h with 200 U of Superscript II (Invitrogen); the second strand was completed first with 40 U Escherichia coli DNA polymerase I (Invitrogen), 12 U E. coli DNA ligase (TaKaRa; BioWhittaker, Taufkirchen, Germany), and 2 U RNase H (TaKaRa) at 16°C for 2 h and then with 10 U T4 DNA polymerase I (TaKaRa) for 5 min at 16°C. .. The reaction was stopped with 10 μl 0.5 M EDTA; cDNA was extracted with phenol-chloroform, and the aqueous phase was recovered by Phase Lock Gel separation (Eppendorf, Hamburg, Germany).

    Construct:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: Library preparation and Illumina sequencing RNA sequencing libraries were constructed in parallel from the six species using TruSeq RNA Sample Prep Kits (Illumina, SanDiego, USA). .. The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA).

    Electrophoresis:

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA). .. Complete biotinylation of the 456-bp target fragment was verified by incubation with streptavidin at 37°C for 10 min followed by electrophoresis on a 1% agarose gel.

    Microarray:

    Article Title: Role of Oxygen Availability in CFTR Expression and Function
    Article Snippet: Paragraph title: Microarray ... Second-strand cDNA was generated with Escherichia coli DNA polymerase I (40 U; Invitrogen) in the presence of 2 U RNase H for 2 hours at 16°C.

    Article Title: hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay †
    Article Snippet: Paragraph title: Oligonucleotide microarray analysis. ... The first strand was synthesized at 42°C for 1 h with 200 U of Superscript II (Invitrogen); the second strand was completed first with 40 U Escherichia coli DNA polymerase I (Invitrogen), 12 U E. coli DNA ligase (TaKaRa; BioWhittaker, Taufkirchen, Germany), and 2 U RNase H (TaKaRa) at 16°C for 2 h and then with 10 U T4 DNA polymerase I (TaKaRa) for 5 min at 16°C.

    Incubation:

    Article Title: Role of Oxygen Availability in CFTR Expression and Function
    Article Snippet: A reaction mix containing 5 μg of total RNA, bacterial RNA controls, 0.5 pmol/μl T7-(dT)24 oligonucleotide primers, and SuperscriptTM II reverse transcriptase (200 U/ml; Invitrogen, Carlsbad, CA) was incubated at 42°C for 1 hour. .. Second-strand cDNA was generated with Escherichia coli DNA polymerase I (40 U; Invitrogen) in the presence of 2 U RNase H for 2 hours at 16°C.

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA). .. Complete biotinylation of the 456-bp target fragment was verified by incubation with streptavidin at 37°C for 10 min followed by electrophoresis on a 1% agarose gel.

    Hybridization:

    Article Title: Transcriptome sequencing of the choroid plexus in schizophrenia
    Article Snippet: Total RNA (1 μg) was subjected to two rounds of hybridization to oligo(dT) beads (Dynal, Waltham, MA, USA). .. The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen).

    Article Title: Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia
    Article Snippet: Total RNA (1 μg) was subjected to two rounds of hybridization to oligo(dT) beads (Dynal, Carlsbad, CA, USA). .. The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen).

    Generated:

    Article Title: Role of Oxygen Availability in CFTR Expression and Function
    Article Snippet: .. Second-strand cDNA was generated with Escherichia coli DNA polymerase I (40 U; Invitrogen) in the presence of 2 U RNase H for 2 hours at 16°C. .. The resulting cDNA was purified and added to an in vitro transcription reaction containing buffer, ATP, GTP, CTP, biotin 11-UTP (Ambion, TX), and enzyme mix.

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: DNA affinity magnetic beads were generated by attaching biotinylated DNA containing the dnaK CIRCE operator sequence of C. trachomatis serovar L2 to streptavidin-conjugated beads by following the method of Gabrielsen and Huet ( ). .. The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA).

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: First-strand cDNA was generated from ∼5 μg of total RNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fermentas) according to manufacturer’s instructions. .. Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis.

    Sequencing:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: Paragraph title: Library preparation and Illumina sequencing ... The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA).

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: DNA affinity magnetic beads were generated by attaching biotinylated DNA containing the dnaK CIRCE operator sequence of C. trachomatis serovar L2 to streptavidin-conjugated beads by following the method of Gabrielsen and Huet ( ). .. The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA).

    Article Title: Transcriptome sequencing of the choroid plexus in schizophrenia
    Article Snippet: Paragraph title: Library preparation and mRNA sequencing ... The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen).

    Article Title: Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia
    Article Snippet: Paragraph title: Library preparation and mRNA sequencing ... The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen).

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: The synthesis was carried out using a biotinylated oligo(dT) fused to the 454 sequencing primer B (5′-biotin-GCCTTGCCAGCCC GCTCAG(T)17 V-3′, where V stands for any base but T). .. Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis.

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Paragraph title: Unbiased amplification of TCR and BCR genes and sequencing ... After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Affinity Purification:

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: Paragraph title: DNA affinity purification of native HrcA. ... The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA).

    RNA Sequencing Assay:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: Library preparation and Illumina sequencing RNA sequencing libraries were constructed in parallel from the six species using TruSeq RNA Sample Prep Kits (Illumina, SanDiego, USA). .. The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA).

    Article Title: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer
    Article Snippet: Paragraph title: RNA-seq library preparation ... Second-strand cDNA synthesis was performed with Escherichia coli DNA Pol I (Life Technologies).

    Article Title: Genome Organization Drives Chromosome Fragility
    Article Snippet: For Nascent RNA-seq, B cell cultures stimulated for 12 hours and 4 million were labeled with 0.5 mM 5-ethynyl uridine (EU) for 30 minutes. .. First-strand cDNA synthesis of the captured nascent RNA was done using SuperScript VILO cDNA synthesis kit (Invitrogen), following AMPure XP purification (1.8X) and elution in 20 µl second-strand cDNA was synthesized with 0.6 mM dNTPs in the presence of 2 units of RNase H (Invitrogen) and 20 units of E. coli DNA polymerase I (Invitrogen) in a total volume of 30 µl for 2.5 hours at 16°C.

    Article Title: Characteristics of cross-hybridization and cross-alignment of expression in pseudo-xenograft samples by RNA-Seq and microarrays
    Article Snippet: .. RNA-Seq mRNA (200 ng) was fragmented at 70 uC for 5 minutes in a fragmentation buffer (Ambion), and converted to first-strand cDNA using Superscript III (Invitrogen); followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep Kit.

    Magnetic Beads:

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: DNA affinity magnetic beads were generated by attaching biotinylated DNA containing the dnaK CIRCE operator sequence of C. trachomatis serovar L2 to streptavidin-conjugated beads by following the method of Gabrielsen and Huet ( ). .. The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA).

    Isolation:

    Article Title: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer
    Article Snippet: Poly(A) RNA from LNCaP and PrEC cells (200 ng) was isolated from total RNA using SeraMag Magnetic Oligo(dT) Beads (Thermo Fisher Scientific). .. Second-strand cDNA synthesis was performed with Escherichia coli DNA Pol I (Life Technologies).

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis.

    Article Title: hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay †
    Article Snippet: Total RNA was isolated from mid-confluent-phase WT and H2KD cells with Trizol reagent (Invitrogen) and further purified using an RNeasy mini kit (QIAGEN). .. The first strand was synthesized at 42°C for 1 h with 200 U of Superscript II (Invitrogen); the second strand was completed first with 40 U Escherichia coli DNA polymerase I (Invitrogen), 12 U E. coli DNA ligase (TaKaRa; BioWhittaker, Taufkirchen, Germany), and 2 U RNase H (TaKaRa) at 16°C for 2 h and then with 10 U T4 DNA polymerase I (TaKaRa) for 5 min at 16°C.

    Labeling:

    Article Title: Genome Organization Drives Chromosome Fragility
    Article Snippet: For Nascent RNA-seq, B cell cultures stimulated for 12 hours and 4 million were labeled with 0.5 mM 5-ethynyl uridine (EU) for 30 minutes. .. First-strand cDNA synthesis of the captured nascent RNA was done using SuperScript VILO cDNA synthesis kit (Invitrogen), following AMPure XP purification (1.8X) and elution in 20 µl second-strand cDNA was synthesized with 0.6 mM dNTPs in the presence of 2 units of RNase H (Invitrogen) and 20 units of E. coli DNA polymerase I (Invitrogen) in a total volume of 30 µl for 2.5 hours at 16°C.

    Article Title: hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay †
    Article Snippet: The first strand was synthesized at 42°C for 1 h with 200 U of Superscript II (Invitrogen); the second strand was completed first with 40 U Escherichia coli DNA polymerase I (Invitrogen), 12 U E. coli DNA ligase (TaKaRa; BioWhittaker, Taufkirchen, Germany), and 2 U RNase H (TaKaRa) at 16°C for 2 h and then with 10 U T4 DNA polymerase I (TaKaRa) for 5 min at 16°C. .. Precipitated cDNA was dissolved in RNase-free water and used to synthesize biotinylated cRNA using the BioArray high yield RNA transcript labeling kit from Enzo Diagnostics (Santa Clara, CA).

    Purification:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA). .. Double-stranded cDNA was purified with a Qiaquick PCR purification kit (Qiagen), and sheared with a nebulizer (Invitrogen, CA, USA) into 200–250 bp fragments.

    Article Title: Role of Oxygen Availability in CFTR Expression and Function
    Article Snippet: Total RNA was purified from HT29 cells (QiaAmp RNeasy kit per manufacturer instructions [Qiagen, Valencia, CA]), and RNA quality was assessed (2100 Bioanalyzer; Agilent, Palo Alto, CA). .. Second-strand cDNA was generated with Escherichia coli DNA polymerase I (40 U; Invitrogen) in the presence of 2 U RNase H for 2 hours at 16°C.

    Article Title: Genome Organization Drives Chromosome Fragility
    Article Snippet: .. First-strand cDNA synthesis of the captured nascent RNA was done using SuperScript VILO cDNA synthesis kit (Invitrogen), following AMPure XP purification (1.8X) and elution in 20 µl second-strand cDNA was synthesized with 0.6 mM dNTPs in the presence of 2 units of RNase H (Invitrogen) and 20 units of E. coli DNA polymerase I (Invitrogen) in a total volume of 30 µl for 2.5 hours at 16°C. ..

    Article Title: hUPF2 Silencing Identifies Physiologic Substrates of Mammalian Nonsense-Mediated mRNA Decay †
    Article Snippet: Total RNA was isolated from mid-confluent-phase WT and H2KD cells with Trizol reagent (Invitrogen) and further purified using an RNeasy mini kit (QIAGEN). .. The first strand was synthesized at 42°C for 1 h with 200 U of Superscript II (Invitrogen); the second strand was completed first with 40 U Escherichia coli DNA polymerase I (Invitrogen), 12 U E. coli DNA ligase (TaKaRa; BioWhittaker, Taufkirchen, Germany), and 2 U RNase H (TaKaRa) at 16°C for 2 h and then with 10 U T4 DNA polymerase I (TaKaRa) for 5 min at 16°C.

    Polymerase Chain Reaction:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA). .. Double-stranded cDNA was purified with a Qiaquick PCR purification kit (Qiagen), and sheared with a nebulizer (Invitrogen, CA, USA) into 200–250 bp fragments.

    Article Title: Gene expression profiling by massively parallel sequencing
    Article Snippet: RNA samples were treated with DNase I (10 units/50 μg of total RNA), and absence of contaminating genomic DNA was confirmed by PCR of two low-expressed genes CG11053 and CG13272 (Supplemental Table 1) from total RNA. .. Double-stranded cDNA was synthesized by addition of 30 U of Escherichia coli DNA polymerase I and 1 U of E. coli ribonuclease H to the first-strand synthesis reaction, following the manufacturer’s (Fermentas) suggested protocol for second-strand cDNA synthesis.

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: In brief, unbiased adaptor-ligation PCR was performed in accordance with previous reports., Total RNA was converted to complementary DNA (cDNA) with Superscript III reverse transcriptase (Invitrogen). .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    cDNA Library Assay:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA). .. The products were then purified and enriched with PCR to create the final sequencing cDNA library.

    Article Title: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer
    Article Snippet: Second-strand cDNA synthesis was performed with Escherichia coli DNA Pol I (Life Technologies). .. The double-stranded cDNA library was further processed following the Illumina Genomic DNA sample preparation protocol, which involved end repair using T4 DNA polymerase, Klenow DNA polymerase, and T4 Polynucleotide kinase followed by a single “A” base addition using Klenow 3′-to-5′ exo− polymerase.

    Article Title: Transcriptome sequencing of the choroid plexus in schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep kit (Illumina, San Diego, CA, USA).

    Article Title: Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep kit (San Diego, CA, USA).

    Article Title: Characteristics of cross-hybridization and cross-alignment of expression in pseudo-xenograft samples by RNA-Seq and microarrays
    Article Snippet: RNA-Seq mRNA (200 ng) was fragmented at 70 uC for 5 minutes in a fragmentation buffer (Ambion), and converted to first-strand cDNA using Superscript III (Invitrogen); followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep Kit.

    Sample Prep:

    Article Title: Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species
    Article Snippet: Library preparation and Illumina sequencing RNA sequencing libraries were constructed in parallel from the six species using TruSeq RNA Sample Prep Kits (Illumina, SanDiego, USA). .. The second strand was synthesized with Escherichia coli DNA Pol I (Invitrogen, CA, USA).

    Article Title: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer
    Article Snippet: Second-strand cDNA synthesis was performed with Escherichia coli DNA Pol I (Life Technologies). .. The double-stranded cDNA library was further processed following the Illumina Genomic DNA sample preparation protocol, which involved end repair using T4 DNA polymerase, Klenow DNA polymerase, and T4 Polynucleotide kinase followed by a single “A” base addition using Klenow 3′-to-5′ exo− polymerase.

    Article Title: Transcriptome sequencing of the choroid plexus in schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep kit (Illumina, San Diego, CA, USA).

    Article Title: Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep kit (San Diego, CA, USA).

    Article Title: Characteristics of cross-hybridization and cross-alignment of expression in pseudo-xenograft samples by RNA-Seq and microarrays
    Article Snippet: RNA-Seq mRNA (200 ng) was fragmented at 70 uC for 5 minutes in a fragmentation buffer (Ambion), and converted to first-strand cDNA using Superscript III (Invitrogen); followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The double stranded cDNA library was further processed by Illumina Genomic DNA Sample Prep Kit.

    RNA Extraction:

    Article Title: Genome Organization Drives Chromosome Fragility
    Article Snippet: Total RNA extraction was performed using TRIzol (Ambion) and 1 µg was rRNA depleted using the NEBNext rRNA Depletion kit (human/mouse/rat) (New England Biosciences), prior to biotinylation by the Click-it reaction (Click-iT Nascent RNA Capture Kit, ThermoFisher ) using manofacture’s specification. .. First-strand cDNA synthesis of the captured nascent RNA was done using SuperScript VILO cDNA synthesis kit (Invitrogen), following AMPure XP purification (1.8X) and elution in 20 µl second-strand cDNA was synthesized with 0.6 mM dNTPs in the presence of 2 units of RNase H (Invitrogen) and 20 units of E. coli DNA polymerase I (Invitrogen) in a total volume of 30 µl for 2.5 hours at 16°C.

    Agarose Gel Electrophoresis:

    Article Title: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer
    Article Snippet: Second-strand cDNA synthesis was performed with Escherichia coli DNA Pol I (Life Technologies). .. The adaptor-ligated library was size-selected by separating on a 4% agarose gel and cutting out the library smear at 200 bp.

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA). .. Complete biotinylation of the 456-bp target fragment was verified by incubation with streptavidin at 37°C for 10 min followed by electrophoresis on a 1% agarose gel.

    Article Title: Transcriptome sequencing of the choroid plexus in schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. Adaptor-ligated library was size selected by electrophoretic separation on a 2% agarose gel and excising of the library smear at 500 bp.

    Article Title: Gene expression profiling by mRNA sequencing reveals increased expression of immune/inflammation-related genes in the hippocampus of individuals with schizophrenia
    Article Snippet: The mRNA was randomly fragmented to between 200 and 700 bp by focused acoustic shearing (Covaris, Woburn, MA, USA) and converted to first strand cDNA using Superscript III (Invitrogen, Carlsbad, CA, USA), followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. Adaptor-ligated library was size selected by electrophotetic separaton on a 2% agarose gel and excising the library smear at 500 bp.

    Article Title: Characteristics of cross-hybridization and cross-alignment of expression in pseudo-xenograft samples by RNA-Seq and microarrays
    Article Snippet: RNA-Seq mRNA (200 ng) was fragmented at 70 uC for 5 minutes in a fragmentation buffer (Ambion), and converted to first-strand cDNA using Superscript III (Invitrogen); followed by second-strand cDNA synthesis using Escherichia coli DNA pol I (Invitrogen). .. The adaptor-ligated library was size-selected by separating on a 4% agarose gel and cutting out the library smear at 200-base-pairs (bp) (625 bp).

    In Vitro:

    Article Title: Role of Oxygen Availability in CFTR Expression and Function
    Article Snippet: Second-strand cDNA was generated with Escherichia coli DNA polymerase I (40 U; Invitrogen) in the presence of 2 U RNase H for 2 hours at 16°C. .. The resulting cDNA was purified and added to an in vitro transcription reaction containing buffer, ATP, GTP, CTP, biotin 11-UTP (Ambion, TX), and enzyme mix.

    Next-Generation Sequencing:

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Unbiased amplification of TCR and BCR genes and sequencing An NGS analysis was performed with the unbiased TCR/BCR repertoire analysis technology developed by Repertoire Genesis, Inc. .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Produced:

    Article Title: Chlamydial GroEL Autoregulates Its Own Expression through Direct Interactions with the HrcA Repressor Protein
    Article Snippet: The C. trachomatis serovar MoPn dnaK CIRCE DNA fragment was produced by digesting the plasmids pMT1173 or pMT1122 (Table ) with the restriction enzymes EcoRI, ApaI, and SacI. .. The DNA fragments with 5′ overhangs were biotinylated with a fill-in reaction by using the Klenow fragment of Escherichia coli DNA polymerase I in the presence of biotin-14-dATP by following the manufacturer's directions (Invitrogen, Carlsbad, CA).

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    Thermo Fisher e coli dna polymerase i
    E Coli Dna Polymerase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dna polymerase i/product/Thermo Fisher
    Average 90 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    e coli dna polymerase i - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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    Thermo Fisher dynazyme ii dna polymerase
    Dynazyme Ii Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynazyme ii dna polymerase/product/Thermo Fisher
    Average 90 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    dynazyme ii dna polymerase - by Bioz Stars, 2020-02
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