e coli dna ligase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e coli dna ligase
    E Coli Dna Ligase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dna ligase/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    e coli dna ligase - by Bioz Stars, 2020-02
    99/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis-Induced Neurodegeneration
    Article Snippet: Loaded microbeads were placed into a flow cell forming a densely packed monolayer. .. Second-strand cDNA synthesis was performed by adding 40 U of DNA polymerase I, 2 U of RNase H, 10 U of Escherichia coli DNA ligase, and 0.5 m m each dNTP in 1× second-strand buffer (Invitrogen) in a final volume of 100 μl for 2 hr at 16°C.

    Amplification:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer. .. After the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy mini column (QIAGEN), fragmented to 50 to 200 nucleotides, and then hybridized to mouse genome U74A v. 2 arrays (Affymetrix).

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Paragraph title: Unbiased amplification of TCR and BCR genes and sequencing ... After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Sample Prep:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: The protocol used for sample preparation and microarray processing is available from Affymetrix (Santa Clara, CA). .. After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer.

    In Vitro:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: .. After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer. .. After the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy mini column (QIAGEN), fragmented to 50 to 200 nucleotides, and then hybridized to mouse genome U74A v. 2 arrays (Affymetrix).

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: RNA was linearly amplified by 2 rounds of in vitro transcription (IVT) by the method of Hunter and colleagues, modified to maintain representation of mRNA levels from small starting quantities of RNA and avoid template-independent amplification (Baugh et al ). .. After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA).

    Chromatography:

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: .. After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA). .. After precipitation, IVT was performed (Ribomax kit; Promega), and the IVT products were purified using a Qiagen RNeasy Mini Column (Qiagen, Valencia, CA).

    Synthesized:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: .. After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer. .. After the cRNA was linearly amplified with T7 polymerase, the biotinylated cRNA was cleaned with an RNeasy mini column (QIAGEN), fragmented to 50 to 200 nucleotides, and then hybridized to mouse genome U74A v. 2 arrays (Affymetrix).

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA). .. After RNAse H addition and annealing of oligo(dT)-T7 primer, second strand was synthesized without E coli ligase.

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen). .. The ds-cDNA was blunted with T4 DNA polymerase (Invitrogen).

    Isolation:

    Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis-Induced Neurodegeneration
    Article Snippet: Second-strand cDNA synthesis was performed by adding 40 U of DNA polymerase I, 2 U of RNase H, 10 U of Escherichia coli DNA ligase, and 0.5 m m each dNTP in 1× second-strand buffer (Invitrogen) in a final volume of 100 μl for 2 hr at 16°C. .. Resuspended, double-stranded cDNA was digested with Dpn II; 3′ Dpn II fragments were isolated with streptavidin-coupled paramagnetic beads (Dynal Biotech, Hamburg, Germany) and released from the beads with a Bsm BI digest.

    Next-Generation Sequencing:

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Unbiased amplification of TCR and BCR genes and sequencing An NGS analysis was performed with the unbiased TCR/BCR repertoire analysis technology developed by Repertoire Genesis, Inc. .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Ligation:

    Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis-Induced Neurodegeneration
    Article Snippet: Short sequences from the free template ends were obtained simultaneously by a fluorescence-based ligation-mediated sequencing method. .. Second-strand cDNA synthesis was performed by adding 40 U of DNA polymerase I, 2 U of RNase H, 10 U of Escherichia coli DNA ligase, and 0.5 m m each dNTP in 1× second-strand buffer (Invitrogen) in a final volume of 100 μl for 2 hr at 16°C.

    Fluorescence:

    Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis-Induced Neurodegeneration
    Article Snippet: Short sequences from the free template ends were obtained simultaneously by a fluorescence-based ligation-mediated sequencing method. .. Second-strand cDNA synthesis was performed by adding 40 U of DNA polymerase I, 2 U of RNase H, 10 U of Escherichia coli DNA ligase, and 0.5 m m each dNTP in 1× second-strand buffer (Invitrogen) in a final volume of 100 μl for 2 hr at 16°C.

    Mouse Assay:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: Briefly, 3 μg purified RNA, extracted from the testes of 16-week-old Tslc1 +/+ and Tslc1 −/− mice, was reverse transcribed with Superscript II reverse transcriptase (Invitrogen), using primer T7-dT24 containing a T7 RNA polymerase promoter. .. After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer.

    Microarray:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: Paragraph title: Oligonucleotide microarray. ... After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer.

    Random Hexamer Labeling:

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA). .. For second-round amplification, random hexamer primers were added to the cRNA and RT was carried out at 37°C (20 minutes), 42°C (20 minutes), 50°C (10 minutes), and 55°C (10 minutes), and heat inactivated at 65°C (15 minutes).

    Polymerase Chain Reaction:

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: In brief, unbiased adaptor-ligation PCR was performed in accordance with previous reports., Total RNA was converted to complementary DNA (cDNA) with Superscript III reverse transcriptase (Invitrogen). .. After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Purification:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: Briefly, 3 μg purified RNA, extracted from the testes of 16-week-old Tslc1 +/+ and Tslc1 −/− mice, was reverse transcribed with Superscript II reverse transcriptase (Invitrogen), using primer T7-dT24 containing a T7 RNA polymerase promoter. .. After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer.

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: .. After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA). .. After precipitation, IVT was performed (Ribomax kit; Promega), and the IVT products were purified using a Qiagen RNeasy Mini Column (Qiagen, Valencia, CA).

    Modification:

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: RNA was linearly amplified by 2 rounds of in vitro transcription (IVT) by the method of Hunter and colleagues, modified to maintain representation of mRNA levels from small starting quantities of RNA and avoid template-independent amplification (Baugh et al ). .. After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA).

    Sequencing:

    Article Title: Tumor Necrosis Factor-Like Weak Inducer of Apoptosis-Induced Neurodegeneration
    Article Snippet: Signatures matching more than one gene (taking into account only nonexpressed sequence tags or expressed sequence tag European Molecular Biology Laboratory database entries) could be detected by clustering all matching sequences, excluding putative sequencing errors and sequence polymorphisms. .. Second-strand cDNA synthesis was performed by adding 40 U of DNA polymerase I, 2 U of RNase H, 10 U of Escherichia coli DNA ligase, and 0.5 m m each dNTP in 1× second-strand buffer (Invitrogen) in a final volume of 100 μl for 2 hr at 16°C.

    Article Title: Next-generation sequencing analysis of the human T-cell and B-cell receptor repertoire diversity before and after hepatitis B vaccination
    Article Snippet: Paragraph title: Unbiased amplification of TCR and BCR genes and sequencing ... After cDNA synthesis, double-stranded (ds)-cDNA was synthesized with Escherichia coli DNA polymerase I (Invitrogen), E. coli DNA ligase (Invitrogen), and RNase H (Invitrogen).

    Staining:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer. .. The stained microarrays were scanned with a GeneArray scanner (Affymetrix), and the signals were calculated with the Affymetrix software Microarray Suite 5.0.

    Binding Assay:

    Article Title: An RNA interference model of RPS19 deficiency in Diamond-Blackfan anemia recapitulates defective hematopoiesis and rescue by dexamethasone: identification of dexamethasone-responsive genes by microarray
    Article Snippet: Briefly, first-round amplification was carried out with oligo(dT)-T7 primers and Superscript II (Invitrogen) in the presence of the single-stranded nucleic acid binding protein T4gp32 (USB) for one hour at 4°C. .. After second-strand synthesis in the presence of DNA polymerase, Escherichia coli RNase H, and E coli DNA ligase (Invitrogen, Cleveland, OH), the DNA was polished with T4 DNA polymerase and purified by phenol/chloroform extraction followed by chromatography on a BioGel P-6 MicroSpin Column (BioRad, Hercules, CA).

    Software:

    Article Title: Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule †
    Article Snippet: After a second strand of cDNA was synthesized using RNase H, Escherichia coli DNA polymerase, and E. coli DNA ligase, in vitro transcription was carried out on the cDNA to produce a biotin-labeled cRNA with a MEGAscript High Yield transcription kit (Ambion, Austin, TX), as recommended by the manufacturer. .. The stained microarrays were scanned with a GeneArray scanner (Affymetrix), and the signals were calculated with the Affymetrix software Microarray Suite 5.0.

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