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Toyobo e coli dh5α
E Coli Dh5α, supplied by Toyobo, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli dh5α/product/Toyobo
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
e coli dh5α - by Bioz Stars, 2020-09
92/100 stars

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Clone Assay:

Article Title: Preclinical Characterization of JTK-853, a Novel Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
Article Snippet: .. The amplified products were ligated to the pCR Blunt II TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen) and transformed into E. coli DH5α (Toyobo, Osaka, Japan). .. Plasmids were purified using a QIAfilter plasmid midikit (Qiagen), and then the nucleoside sequence was determined using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) and ABI Prism 3100 genetic analyzer (Applied Biosystems).

Article Title: Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts
Article Snippet: .. The PCR product was cloned using pGEM-T Easy Vector Systems (Promega) and E. coli DH5α (TOYOBO, Co., Osaka). .. DNA for sequencing was directly amplified from the E. coli colony by PCR using Blend Taq Plus.

Amplification:

Article Title: Preclinical Characterization of JTK-853, a Novel Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
Article Snippet: .. The amplified products were ligated to the pCR Blunt II TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen) and transformed into E. coli DH5α (Toyobo, Osaka, Japan). .. Plasmids were purified using a QIAfilter plasmid midikit (Qiagen), and then the nucleoside sequence was determined using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) and ABI Prism 3100 genetic analyzer (Applied Biosystems).

Ligation:

Article Title: Systematic genome sequence differences among leaf cells within individual trees
Article Snippet: .. Competent cells of E. coli DH5α (Toyobo Co. Ltd., Osaka, Japan) were transformed with the ligation product. .. Transformed cells were cultivated on LB agar plates (1% tryptone, 0.5% yeast extract, 1% NaCl [pH 7.0], and 1.5% agar) supplemented with ampicillin (10 mg in 200 ml of LB media), 20 μl X-Gal (50 mg ml-1 in dimethyformamide), and 100 μl of 0.1 M IPTG (isopropylthio-β-galactoside).

Purification:

Article Title: Discovery of Two β-1,2-Mannoside Phosphorylases Showing Different Chain-Length Specificities from Thermoanaerobacter sp. X-514
Article Snippet: .. The expression plasmids were propagated in Escherichia coli DH5α (Toyobo), purified using a FastGene Plasmid Mini Kit (Nippon Genetics Co.), and verified via sequencing (Operon Biotechnologies, Tokyo, Japan). .. An E. coli Rosetta 2 (DE3) (Novagen) transformant harboring each of the expression plasmids was grown at 37°C in 200 mL of Luria-Bertani medium (1% tryptone, 0.5% yeast extract, and 0.5% NaCl) containing 50 µg·mL−1 kanamycin and 30 µg·mL−1 chloramphenicol until the absorbance reached 0.6 at 600 nm.

Polymerase Chain Reaction:

Article Title: Preclinical Characterization of JTK-853, a Novel Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
Article Snippet: .. The amplified products were ligated to the pCR Blunt II TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen) and transformed into E. coli DH5α (Toyobo, Osaka, Japan). .. Plasmids were purified using a QIAfilter plasmid midikit (Qiagen), and then the nucleoside sequence was determined using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) and ABI Prism 3100 genetic analyzer (Applied Biosystems).

Article Title: Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts
Article Snippet: .. The PCR product was cloned using pGEM-T Easy Vector Systems (Promega) and E. coli DH5α (TOYOBO, Co., Osaka). .. DNA for sequencing was directly amplified from the E. coli colony by PCR using Blend Taq Plus.

Expressing:

Article Title: Discovery of Two β-1,2-Mannoside Phosphorylases Showing Different Chain-Length Specificities from Thermoanaerobacter sp. X-514
Article Snippet: .. The expression plasmids were propagated in Escherichia coli DH5α (Toyobo), purified using a FastGene Plasmid Mini Kit (Nippon Genetics Co.), and verified via sequencing (Operon Biotechnologies, Tokyo, Japan). .. An E. coli Rosetta 2 (DE3) (Novagen) transformant harboring each of the expression plasmids was grown at 37°C in 200 mL of Luria-Bertani medium (1% tryptone, 0.5% yeast extract, and 0.5% NaCl) containing 50 µg·mL−1 kanamycin and 30 µg·mL−1 chloramphenicol until the absorbance reached 0.6 at 600 nm.

Sequencing:

Article Title: Discovery of Two β-1,2-Mannoside Phosphorylases Showing Different Chain-Length Specificities from Thermoanaerobacter sp. X-514
Article Snippet: .. The expression plasmids were propagated in Escherichia coli DH5α (Toyobo), purified using a FastGene Plasmid Mini Kit (Nippon Genetics Co.), and verified via sequencing (Operon Biotechnologies, Tokyo, Japan). .. An E. coli Rosetta 2 (DE3) (Novagen) transformant harboring each of the expression plasmids was grown at 37°C in 200 mL of Luria-Bertani medium (1% tryptone, 0.5% yeast extract, and 0.5% NaCl) containing 50 µg·mL−1 kanamycin and 30 µg·mL−1 chloramphenicol until the absorbance reached 0.6 at 600 nm.

Transformation Assay:

Article Title: Contribution of the 8-Methoxy Group to the Activity of Gatifloxacin against Type II Topoisomerases of Streptococcus pneumoniae
Article Snippet: .. The DNA fragments of gyrA , parC , and parE and pGEX-2T (Amersham Pharmacia Biotech) were digested with Bam HI, ligated, and transformed into E. coli DH5α (Toyobo, Tokyo, Japan). .. The DNA fragments of gyrB and pGEX-2T were digested with Eco RI and Bam HI, ligated, and transformed into E. coli DH5α.

Article Title: Preclinical Characterization of JTK-853, a Novel Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
Article Snippet: .. The amplified products were ligated to the pCR Blunt II TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen) and transformed into E. coli DH5α (Toyobo, Osaka, Japan). .. Plasmids were purified using a QIAfilter plasmid midikit (Qiagen), and then the nucleoside sequence was determined using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) and ABI Prism 3100 genetic analyzer (Applied Biosystems).

Article Title: Systematic genome sequence differences among leaf cells within individual trees
Article Snippet: .. Competent cells of E. coli DH5α (Toyobo Co. Ltd., Osaka, Japan) were transformed with the ligation product. .. Transformed cells were cultivated on LB agar plates (1% tryptone, 0.5% yeast extract, 1% NaCl [pH 7.0], and 1.5% agar) supplemented with ampicillin (10 mg in 200 ml of LB media), 20 μl X-Gal (50 mg ml-1 in dimethyformamide), and 100 μl of 0.1 M IPTG (isopropylthio-β-galactoside).

Recombinant:

Article Title: Membrane-displayed somatostatin activates somatostatin receptor subtype-2 heterologously produced in Saccharomyces cerevisiae
Article Snippet: .. Escherichia coli DH5α (Toyobo, Osaka, Japan) was used as the host for recombinant DNA manipulation and grown in Luria–Bertani medium (1% [w/v] tryptone, 0.5% [w/v] yeast extract, and 0.5% [w/v] sodium chloride) containing 100 μg·mL-1 ampicillin. .. Expression vectors PCR was performed using KOD-Plus-DNA polymerase (Toyobo).

Article Title: Construction of a simple biocatalyst using psychrophilic bacterial cells and its application for efficient 3-hydroxypropionaldehyde production from glycerol
Article Snippet: .. E. coli DH5α (TOYOBO, Japan) was used for the construction of the recombinant plasmids. ..

Plasmid Preparation:

Article Title: Preclinical Characterization of JTK-853, a Novel Nonnucleoside Inhibitor of the Hepatitis C Virus RNA-Dependent RNA Polymerase
Article Snippet: .. The amplified products were ligated to the pCR Blunt II TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen) and transformed into E. coli DH5α (Toyobo, Osaka, Japan). .. Plasmids were purified using a QIAfilter plasmid midikit (Qiagen), and then the nucleoside sequence was determined using a BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA) and ABI Prism 3100 genetic analyzer (Applied Biosystems).

Article Title: Discovery of Two β-1,2-Mannoside Phosphorylases Showing Different Chain-Length Specificities from Thermoanaerobacter sp. X-514
Article Snippet: .. The expression plasmids were propagated in Escherichia coli DH5α (Toyobo), purified using a FastGene Plasmid Mini Kit (Nippon Genetics Co.), and verified via sequencing (Operon Biotechnologies, Tokyo, Japan). .. An E. coli Rosetta 2 (DE3) (Novagen) transformant harboring each of the expression plasmids was grown at 37°C in 200 mL of Luria-Bertani medium (1% tryptone, 0.5% yeast extract, and 0.5% NaCl) containing 50 µg·mL−1 kanamycin and 30 µg·mL−1 chloramphenicol until the absorbance reached 0.6 at 600 nm.

Article Title: Amplification of a plasmid bearing a mammalian replication initiation region in chromosomal and extrachromosomal contexts
Article Snippet: .. The PCR product was cloned using pGEM-T Easy Vector Systems (Promega) and E. coli DH5α (TOYOBO, Co., Osaka). .. DNA for sequencing was directly amplified from the E. coli colony by PCR using Blend Taq Plus.

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    https://www.bioz.com/result/e coli dh5α/product/Toyobo
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    Price from $9.99 to $1999.99
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      Buy from Supplier

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