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Stratagene e coli bj5183 cells
E Coli Bj5183 Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e coli bj5183 cells/product/Stratagene
Average 89 stars, based on 9 article reviews
Price from $9.99 to $1999.99
e coli bj5183 cells - by Bioz Stars, 2020-09
89/100 stars

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Clone Assay:

Article Title: Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human
Article Snippet: .. Briefly, the mutant L39stop cDNA was cloned into the shuttle vector pAdTrack-CMV (gift from Roger J. Hajjar, Harvard Medical School, Boston, Massachusetts, USA), linearized with restriction endonuclease Pme I, and cotransfected into E. coli BJ5183 cells (Stratagene, La Jolla, California, USA) with the adenoviral backbone plasmid pAdEasy-1 (gift from Bert Vogelstein, The Johns Hopkins Oncology Center, Baltimore, Maryland, USA). ..

Mutagenesis:

Article Title: Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human
Article Snippet: .. Briefly, the mutant L39stop cDNA was cloned into the shuttle vector pAdTrack-CMV (gift from Roger J. Hajjar, Harvard Medical School, Boston, Massachusetts, USA), linearized with restriction endonuclease Pme I, and cotransfected into E. coli BJ5183 cells (Stratagene, La Jolla, California, USA) with the adenoviral backbone plasmid pAdEasy-1 (gift from Bert Vogelstein, The Johns Hopkins Oncology Center, Baltimore, Maryland, USA). ..

Construct:

Article Title: A potent malaria vaccine based on adenovirus with dual modifications at Hexon and pVII
Article Snippet: .. The recombinant shuttle vector was linearized by Pme I digestion and used for homologous recombination with pAdEasy-1 vector in E. coli BJ5183 cells (Stratagene) to construct PfCSP recombinant adenovirus genome, AdPfCSP. .. Insertion of (NANP)n in Hexon was done by replacing HVR1 sequence (from 138 to 164 a.a.) with the repeat sequence.

Generated:

Article Title: Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness
Article Snippet: .. The pXK3.1 plasmid contains two long arms complementary to the adenoviral genome at both fiber extremes. pAdFO was generated by homologous recombination of the pXK3.1-FO with the genome of the wild-type adenovirus in E. coli BJ5183 cells (Stratagene, Wilmington, NC) as described in . .. Adwt-EGFP was generated following a protocol adapted from Stanton et al. based on homologous recombination in bacteria using double selection with the rpsLNeo cassette .

Article Title: MiR-148a- and miR-216a-regulated Oncolytic Adenoviruses Targeting Pancreatic Tumors Attenuate Tissue Damage Without Perturbation of miRNA Activity
Article Snippet: .. Adenovirus generation. pAdeno-miRTs (pAdeno-miR148aT, pAdeno-miR148a148aT, and pAdeno-miR148a216aT) were generated by homologous recombination of the pEndK-miRTs (pEndK-miR148aT, pEndK-miR148a148aT, and pEndK-miR148a216aT) with the genome of the serotype 5 wild-type adenovirus (Ad-wt) in E. Coli BJ5183 cells (Stratagene) as described in ref. . .. The resulting pAdeno-miRTs were transfected to HEK293 cells and following several cycles of infection the viruses in A549 cells were purified by cesium chloride gradient to obtain Ad-miR148aT, Ad-miR148a148aT and Ad-miR148a216a.

Homologous Recombination:

Article Title: Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness
Article Snippet: .. The pXK3.1 plasmid contains two long arms complementary to the adenoviral genome at both fiber extremes. pAdFO was generated by homologous recombination of the pXK3.1-FO with the genome of the wild-type adenovirus in E. coli BJ5183 cells (Stratagene, Wilmington, NC) as described in . .. Adwt-EGFP was generated following a protocol adapted from Stanton et al. based on homologous recombination in bacteria using double selection with the rpsLNeo cassette .

Article Title: A potent malaria vaccine based on adenovirus with dual modifications at Hexon and pVII
Article Snippet: .. The recombinant shuttle vector was linearized by Pme I digestion and used for homologous recombination with pAdEasy-1 vector in E. coli BJ5183 cells (Stratagene) to construct PfCSP recombinant adenovirus genome, AdPfCSP. .. Insertion of (NANP)n in Hexon was done by replacing HVR1 sequence (from 138 to 164 a.a.) with the repeat sequence.

Article Title: MiR-148a- and miR-216a-regulated Oncolytic Adenoviruses Targeting Pancreatic Tumors Attenuate Tissue Damage Without Perturbation of miRNA Activity
Article Snippet: .. Adenovirus generation. pAdeno-miRTs (pAdeno-miR148aT, pAdeno-miR148a148aT, and pAdeno-miR148a216aT) were generated by homologous recombination of the pEndK-miRTs (pEndK-miR148aT, pEndK-miR148a148aT, and pEndK-miR148a216aT) with the genome of the serotype 5 wild-type adenovirus (Ad-wt) in E. Coli BJ5183 cells (Stratagene) as described in ref. . .. The resulting pAdeno-miRTs were transfected to HEK293 cells and following several cycles of infection the viruses in A549 cells were purified by cesium chloride gradient to obtain Ad-miR148aT, Ad-miR148a148aT and Ad-miR148a216a.

Transformation Assay:

Article Title: Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis *Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis * S⃞
Article Snippet: .. The resultant plasmids (containing either HSL, ATGL, or no insert) were linearized by PmeI digestion and subsequently transformed into Escherichia coli BJ5183 cells containing an adenoviral backbone plasmid, pAdEasy-1 (Stratagene), to generate recombinant plasmids. .. Recombinant plasmids were isolated, and DNA was purified, linearized, and transfected into 293 cells (ATCC) to obtain viral plaques.

Article Title: Modulation of Ion Channels and Synaptic Transmission by a Human Sensory Neuron-Specific G-Protein-Coupled Receptor, SNSR4/mrgX1, Heterologously Expressed in Cultured Rat Neurons
Article Snippet: .. The resultant plasmid (pAdTrack-CMV-hSNSR4) was linearized with PmeI and electroporated into Escherichia coli BJ5183 cells that had been transformed with pAdEasy-1, an adenoviral backbone plasmid (Stratagene). .. Recombination between these two plasmids was confirmed by restriction analysis and sequencing.

Recombinant:

Article Title: A potent malaria vaccine based on adenovirus with dual modifications at Hexon and pVII
Article Snippet: .. The recombinant shuttle vector was linearized by Pme I digestion and used for homologous recombination with pAdEasy-1 vector in E. coli BJ5183 cells (Stratagene) to construct PfCSP recombinant adenovirus genome, AdPfCSP. .. Insertion of (NANP)n in Hexon was done by replacing HVR1 sequence (from 138 to 164 a.a.) with the repeat sequence.

Article Title: Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis *Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis * S⃞
Article Snippet: .. The resultant plasmids (containing either HSL, ATGL, or no insert) were linearized by PmeI digestion and subsequently transformed into Escherichia coli BJ5183 cells containing an adenoviral backbone plasmid, pAdEasy-1 (Stratagene), to generate recombinant plasmids. .. Recombinant plasmids were isolated, and DNA was purified, linearized, and transfected into 293 cells (ATCC) to obtain viral plaques.

Plasmid Preparation:

Article Title: Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness
Article Snippet: .. The pXK3.1 plasmid contains two long arms complementary to the adenoviral genome at both fiber extremes. pAdFO was generated by homologous recombination of the pXK3.1-FO with the genome of the wild-type adenovirus in E. coli BJ5183 cells (Stratagene, Wilmington, NC) as described in . .. Adwt-EGFP was generated following a protocol adapted from Stanton et al. based on homologous recombination in bacteria using double selection with the rpsLNeo cassette .

Article Title: A potent malaria vaccine based on adenovirus with dual modifications at Hexon and pVII
Article Snippet: .. The recombinant shuttle vector was linearized by Pme I digestion and used for homologous recombination with pAdEasy-1 vector in E. coli BJ5183 cells (Stratagene) to construct PfCSP recombinant adenovirus genome, AdPfCSP. .. Insertion of (NANP)n in Hexon was done by replacing HVR1 sequence (from 138 to 164 a.a.) with the repeat sequence.

Article Title: Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis *Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis * S⃞
Article Snippet: .. The resultant plasmids (containing either HSL, ATGL, or no insert) were linearized by PmeI digestion and subsequently transformed into Escherichia coli BJ5183 cells containing an adenoviral backbone plasmid, pAdEasy-1 (Stratagene), to generate recombinant plasmids. .. Recombinant plasmids were isolated, and DNA was purified, linearized, and transfected into 293 cells (ATCC) to obtain viral plaques.

Article Title: Construction of a pIX-modified Adenovirus Vector Able to Effectively Bind to Nanoantibodies for Targeting
Article Snippet: .. This plasmid was linearized by restriction digestion with PmeI and cotransformed together with the pAdEasy-1 plasmid into E. coli BJ5183 cells as described in the AdEasy adenoviral vector system (Stratagene, USA). .. The pAd5-EGFPpIX- ER plasmid was obtained as a result of homologous recombination.

Article Title: Modulation of Ion Channels and Synaptic Transmission by a Human Sensory Neuron-Specific G-Protein-Coupled Receptor, SNSR4/mrgX1, Heterologously Expressed in Cultured Rat Neurons
Article Snippet: .. The resultant plasmid (pAdTrack-CMV-hSNSR4) was linearized with PmeI and electroporated into Escherichia coli BJ5183 cells that had been transformed with pAdEasy-1, an adenoviral backbone plasmid (Stratagene). .. Recombination between these two plasmids was confirmed by restriction analysis and sequencing.

Article Title: Human phospholamban null results in lethal dilated cardiomyopathy revealing a critical difference between mouse and human
Article Snippet: .. Briefly, the mutant L39stop cDNA was cloned into the shuttle vector pAdTrack-CMV (gift from Roger J. Hajjar, Harvard Medical School, Boston, Massachusetts, USA), linearized with restriction endonuclease Pme I, and cotransfected into E. coli BJ5183 cells (Stratagene, La Jolla, California, USA) with the adenoviral backbone plasmid pAdEasy-1 (gift from Bert Vogelstein, The Johns Hopkins Oncology Center, Baltimore, Maryland, USA). ..

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    Stratagene escherichia coli bj5183 cells
    Escherichia Coli Bj5183 Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bj5183 cells/product/Stratagene
    Average 88 stars, based on 5 article reviews
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      Buy from Supplier

    85
    Stratagene escherichia coli strain bj5183 ad1 competent cells
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    Stratagene packaging e coli bj5183 cells
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    https://www.bioz.com/result/packaging e coli bj5183 cells/product/Stratagene
    Average 85 stars, based on 1 article reviews
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    packaging e coli bj5183 cells - by Bioz Stars, 2020-09
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      Buy from Supplier

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    Stratagene e coli bj5183 competent cells
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    https://www.bioz.com/result/e coli bj5183 competent cells/product/Stratagene
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    e coli bj5183 competent cells - by Bioz Stars, 2020-09
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      Buy from Supplier

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