dynamag 2 magnet  (Thermo Fisher)


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    Name:
    DynaMag 2 Magnet
    Description:
    The DynaMag 2 magnet is optimized for efficient magnetic separation of all types of Dynabeads range 1 4 5 µm in diameter in small 2 mL sample volumes The DynaMag 2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design • Optimal working volume 10 2 000 µL• Holds up to 16 standard 1 5 2 mL microcentrifuge tubes in numbered spaces• Good control and visibility of your sample• Top rack can be quickly removed from the magnet in the base ready for resuspension vortexing rotation or manual sample shakingFlexible and ergonomic designThe magnet can be used with all types of Dynabeads for efficient isolation of proteins and nucleic acids The sample rack can be easily removed from the base magnet for resuspension vortexing rotation or manual sample shaking A center pin in the rack ensures equal vortexing of all tubes The rack can also be removed and used to store tubes The 16 numbered sample spaces make this magnet ideal for parallel handling of multiple samples With 8 tube positions on each side of the rack you can easily scale your protocols to accommodate 96 well plate reactions Product notes• This magnet is not compatible with the MX4 Mixer• The removable sample rack is also available as a separate item Cat No 123 22D Purchasing an additional sample rack minimizes the need to move tubes between racks The rack can be placed on the bench in the refrigerator or in the incubator Learn more about Dynabeads products• Find Dynabeads products for a whole range of applications • Find other magnets for Dynabeads separations
    Catalog Number:
    12321d
    Price:
    None
    Applications:
    Cell Activation & Expansion|Cell Analysis|Cell Isolation|DNA & RNA Purification & Analysis|DNA Extraction|Immunoprecipitation|Magnets for Cell Isolation|Protein Assays and Analysis|Protein Biology|Protein Purification & Isolation|Sequence-Specific DNA or RNA Purification|Sequencing Reaction Purification
    Category:
    Instruments and Equipment
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    Structured Review

    Thermo Fisher dynamag 2 magnet
    The DynaMag 2 magnet is optimized for efficient magnetic separation of all types of Dynabeads range 1 4 5 µm in diameter in small 2 mL sample volumes The DynaMag 2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design • Optimal working volume 10 2 000 µL• Holds up to 16 standard 1 5 2 mL microcentrifuge tubes in numbered spaces• Good control and visibility of your sample• Top rack can be quickly removed from the magnet in the base ready for resuspension vortexing rotation or manual sample shakingFlexible and ergonomic designThe magnet can be used with all types of Dynabeads for efficient isolation of proteins and nucleic acids The sample rack can be easily removed from the base magnet for resuspension vortexing rotation or manual sample shaking A center pin in the rack ensures equal vortexing of all tubes The rack can also be removed and used to store tubes The 16 numbered sample spaces make this magnet ideal for parallel handling of multiple samples With 8 tube positions on each side of the rack you can easily scale your protocols to accommodate 96 well plate reactions Product notes• This magnet is not compatible with the MX4 Mixer• The removable sample rack is also available as a separate item Cat No 123 22D Purchasing an additional sample rack minimizes the need to move tubes between racks The rack can be placed on the bench in the refrigerator or in the incubator Learn more about Dynabeads products• Find Dynabeads products for a whole range of applications • Find other magnets for Dynabeads separations
    https://www.bioz.com/result/dynamag 2 magnet/product/Thermo Fisher
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    dynamag 2 magnet - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis
    Article Snippet: Antibody capture, cell isolation, and washes were done in PBS (pH 7.2) containing 0.5% BSA (Fraction V) and 2 mM EDTA. .. Dynabead-CD31 capture, BMEC isolations, and washes were done using a DynaMag-2 magnet (Life Technologies).

    Centrifugation:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: The lysate was pre-cleared by centrifugation twice at 20,000 g for 10 min at 4°C, and then diluted with equal volume of extraction buffer without Triton X-100. .. The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Blocking Assay:

    Article Title: Molecular Dissection of Chromatin Maturation Via Click Chemistry
    Article Snippet: PRN2615, Fisher Scientific) 50× Danhard’s blocking buffer (Cat.No. .. 12321D, Life Technologies) QSonica, sonicator (Cat.No., QSonica) Cell lifter (Cat.No.

    Article Title: The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
    Article Snippet: RT-10F, RT-20F, RT-200F, and RT-1000F) Pre-sterilized, RNase-free gel loading pipette tips (National Scientific, cat. no. MN520R-LRS) Refrigerated microcentrifuge 5430R (VWR, cat. no. 97027-866) Optima TLX Ultracentrifuge (Beckman, cat. no. 361545) TLA 100.3 rotor (Beckman, cat. no. 349481) Dry block heater (VWR, cat. no. 12621-104) Dry block for microfuge tubes (VWR, cat. no. 13259-002) Mini-Cell polyacrylamide gel box (Invitrogen, cat. no. EI0001) Electrophoresis power supply (VWR, cat. no. 27370-265) DarkReader (Clare Chemical Research, cat. no. DR46B). .. Thermal cycler (Bio-Rad, cat. no. 170-9713) DynaMag-2 separation rack (Invitrogen, cat. no. 12321D) ThermoMixer (VWR, cat. no. 21516-170) 2100 BioAnalyzer (Agilent Technologies, cat. no. G2940CA) Genome Analyzer II or HiSeq 2000 (Illumina) Computer hardware – a 64-bit computer running Linux with at least 4 GB of RAM for the manual analysis options.

    Electrophoresis:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100). .. The bonded proteins were eluted with 50 μL 2 × SDS loading buffer by incubating at 95°C for 10 min. For western blot analysis, proteins were separated by SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane (Merck Millipore Corporation, Germany) by semi-dry transfer cell (Bio-Rad Laboratories, USA).

    Article Title: The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
    Article Snippet: RT-10F, RT-20F, RT-200F, and RT-1000F) Pre-sterilized, RNase-free gel loading pipette tips (National Scientific, cat. no. MN520R-LRS) Refrigerated microcentrifuge 5430R (VWR, cat. no. 97027-866) Optima TLX Ultracentrifuge (Beckman, cat. no. 361545) TLA 100.3 rotor (Beckman, cat. no. 349481) Dry block heater (VWR, cat. no. 12621-104) Dry block for microfuge tubes (VWR, cat. no. 13259-002) Mini-Cell polyacrylamide gel box (Invitrogen, cat. no. EI0001) Electrophoresis power supply (VWR, cat. no. 27370-265) DarkReader (Clare Chemical Research, cat. no. DR46B). .. Thermal cycler (Bio-Rad, cat. no. 170-9713) DynaMag-2 separation rack (Invitrogen, cat. no. 12321D) ThermoMixer (VWR, cat. no. 21516-170) 2100 BioAnalyzer (Agilent Technologies, cat. no. G2940CA) Genome Analyzer II or HiSeq 2000 (Illumina) Computer hardware – a 64-bit computer running Linux with at least 4 GB of RAM for the manual analysis options.

    Microarray:

    Article Title: Loss of Androgen Receptor Promotes Adipogensis but Suppresses Osteogenesis in Bone Marrow Stromal Cells
    Article Snippet: Paragraph title: Microarray analysis ... After incubation, the samples were put into DynaMag™-2 Magnet (Invitrogen) for 2 minutes and the bound beads were moved to each side.

    Incubation:

    Article Title: Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis
    Article Snippet: In short, 10 μl of sheep anti-rat IgG Dynabeads were incubated with 1 μg of CD31 antibody (per isolation) for 30 min at 4 °C, washed three times, added to lineage-depleted WBM in a final volume of 1 ml, and incubated for 30 min at 4 °C. .. Dynabead-CD31 capture, BMEC isolations, and washes were done using a DynaMag-2 magnet (Life Technologies).

    Article Title: Lung-Derived Factors Mediate Breast Cancer Cell Migration through CD44 Receptor-Ligand Interactions in a Novel Ex Vivo System for Analysis of Organ-Specific Soluble Proteins 1 System for Analysis of Organ-Specific Soluble Proteins 1 2
    Article Snippet: Bead-antibody complexes were incubated with lung-CM or basal media ( N = 3 per condition) for 30 minutes at RT. .. Resulting bead-antibody-antigen complexes were removed using a DynaMag-2 magnet (Invitrogen), and depleted media were assessed by Quantikine ELISA kits specific for mouse SELL or OPN (R & D Systems).

    Article Title: Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
    Article Snippet: Beads without capture antibody, as well as beads coated with IgG isotype control to correct for Fc binding, were also incubated with PPP as controls. .. A DynaMag-2 magnet (Invitrogen, Carlsbad, CA) was used to separate the Dynabeads coated with CD42b linked to microparticles from the PPP.

    Article Title: Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
    Article Snippet: The microvascular fraction was incubated with Dynabeads® CD31 (Thermo Fisher Scientific) for 20 min at 4°C to isolate ECs, following manufacturer’s instructions. .. The EC-bound beads were captured using the DynaMag™-2 Magnet (Thermo Fisher Scientific) and washed three times with PBS.

    Article Title: Loss of Androgen Receptor Promotes Adipogensis but Suppresses Osteogenesis in Bone Marrow Stromal Cells
    Article Snippet: .. After incubation, the samples were put into DynaMag™-2 Magnet (Invitrogen) for 2 minutes and the bound beads were moved to each side. .. The confluent cultured BMSCs were lysed to extract mRNA, and 10 μg fresh mRNA used to determine the whole gene changes via mouse affymetrix microarray.

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: Twenty microliter of Pierce Protein A Magnetic Beads (Thermo Fisher Scientific) coupled with 10 μg anti-GFP polyclonal antibody (custom made) were added to each protein extract and incubated at 4°C for 1 h with rotation. .. The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Article Title: Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment
    Article Snippet: Dynabeads (Thermo Fisher) were added and 1 mg protein was incubated overnight at 4°C in the presence of a specific antibody against LCN-2 (R & D, MAB1757). .. Beads were precipitated using the DynaMag-2 magnet (Thermo Fisher) and washed three times with IP buffer.

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: The third day, culture medium was discarded, cells were washed once with 2 ml Phosphate Buffer Saline without calcium and magnesium (PBS), incubated for 5 to 10 min in 900 μl of lysis buffer, and scraped. .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification.

    Article Title: Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
    Article Snippet: Each incubation step was performed in a thermomixer (Eppendorf) with shaking at 1150 rpm at 25°C. .. Supernatants of each step were removed by applying the reaction to a DynaMag-2 magnet (ThermoFisher Scientific) to isolate the DNA coupled to magnetic streptavidin beads from the supernatant.

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Freshly washed anti-FLAG M2 magnetic beads (30 μl slurry per sample) were added and the samples were incubated with end-over-end rotation for 3 hours at 4°C. .. Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Expressing:

    Article Title: Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis
    Article Snippet: Dynabead-CD31 capture, BMEC isolations, and washes were done using a DynaMag-2 magnet (Life Technologies). .. To select for Akt1 -expressing BMECs, cultures were starved for seven days in serum- and cytokine-free StemSpan SFEM (StemCell Technologies, Inc.) media.

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: For each sample, we used as control cells transfected with 1 μg pCNeo vector expressing the same protein than the HaloTag vector and the same two GPCA vectors. .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification.

    Western Blot:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: Paragraph title: Co-immunoprecipitation assay and western blot analysis ... The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Recombinase Polymerase Amplification:

    Article Title: Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
    Article Snippet: Supernatants of each step were removed by applying the reaction to a DynaMag-2 magnet (ThermoFisher Scientific) to isolate the DNA coupled to magnetic streptavidin beads from the supernatant. .. The Mcm2–7 loading step was done in a volume of 10 µL and incubated for 30 min. After removal of the supernatant, DDK phosphorylation was performed as described previously ( ) in a 10-µL reaction volume for 25 min. After removal of the DDK reaction supernatant, the following amounts of protein were added to the DDK phosphorylated Mcm2–7: 1 pmol of Cdc28/Clb5, 0.3 pmol of DDK, 1 pmol of Sld3/Sld7, 5 pmol of Cdc45, 2 pmol of Sld2, 0.6 pmol of Dpb11, 5 pmol of GINS, 0.15 pmol of Mcm10 (or as indicated), 1.85 pmol of Pol ε, and 2 pmol of RPA.

    Hybridization:

    Article Title: Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
    Article Snippet: SeqCap Pure capture beads (Roche/NimbleGen) were washed twice, mixed with the hybridization mix, and kept at 47°C for 45 min with vortexing for 10 s every 10 to 15 min. .. The streptavidin capture beads complexed with biotinylated VirCapSeq-VERT probes were trapped (DynaMag-2 magnet; Thermo, Fisher) and washed once at 47°C and then twice more at room temperature with wash buffers of increasing stringency.

    Electron Microscopy:

    Article Title: Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
    Article Snippet: Paragraph title: Transmission electron microscopy (TEM) ... A DynaMag-2 magnet (Invitrogen, Carlsbad, CA) was used to separate the Dynabeads coated with CD42b linked to microparticles from the PPP.

    Transfection:

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: For each sample, we used as control cells transfected with 1 μg pCNeo vector expressing the same protein than the HaloTag vector and the same two GPCA vectors. .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification.

    Decantation Assay:

    Article Title: Evaluation of Targeted Delivery to the Brain Using Magnetic Immunoliposomes and Magnetic Force
    Article Snippet: .. Excess lipid was removed by triple magnetic decantation using DynaMAG™-2 magnet (Invitrogen, Oxford, UK), followed by resuspension in HEPES buffer. .. Synthesis of OX26-MAb-Conjugated Magnetic Immunotoliposomes Using the SATA Method Magnetic OX26 immunoliposomes were produced by transferring OX26 coupled to MAL-PEG2000-DSPE into magnetic liposomes ( ) [ ].

    Protease Inhibitor:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: Total proteins from 0.3 g tissue powder were extracted with 0.6 mL extraction buffer (20 mM HEPES, pH7.5, 40 mM KCl, 1 mM EDTA, 1% TritonX-100 and protease inhibitor cocktail tablets from Roche). .. The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Transferring:

    Article Title: The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
    Article Snippet: RT-10F, RT-20F, RT-200F, and RT-1000F) Pre-sterilized, RNase-free gel loading pipette tips (National Scientific, cat. no. MN520R-LRS) Refrigerated microcentrifuge 5430R (VWR, cat. no. 97027-866) Optima TLX Ultracentrifuge (Beckman, cat. no. 361545) TLA 100.3 rotor (Beckman, cat. no. 349481) Dry block heater (VWR, cat. no. 12621-104) Dry block for microfuge tubes (VWR, cat. no. 13259-002) Mini-Cell polyacrylamide gel box (Invitrogen, cat. no. EI0001) Electrophoresis power supply (VWR, cat. no. 27370-265) DarkReader (Clare Chemical Research, cat. no. DR46B). .. Thermal cycler (Bio-Rad, cat. no. 170-9713) DynaMag-2 separation rack (Invitrogen, cat. no. 12321D) ThermoMixer (VWR, cat. no. 21516-170) 2100 BioAnalyzer (Agilent Technologies, cat. no. G2940CA) Genome Analyzer II or HiSeq 2000 (Illumina) Computer hardware – a 64-bit computer running Linux with at least 4 GB of RAM for the manual analysis options.

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded. .. Following the last wash, the samples were quickly centrifuged and the last drops of liquid removed with a fine pipette.

    Cell Culture:

    Article Title: Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis
    Article Snippet: Dynabead-CD31 capture, BMEC isolations, and washes were done using a DynaMag-2 magnet (Life Technologies). .. C57BL/6J and Tie2::IκB-SS BMECs were cultured in low glucose DMEM (Life Technologies) and Ham's F-12 (CellGro) (1:1 ratio), supplemented with 20% heat-inactivated FBS, antibiotic-antimycotic (CellGro), non-essential amino acids (CellGro), 10 mM HEPES (CellGro), 100 μg ml−1 heparin (Sigma-Aldrich) and 50 μg ml−1 endothelial mitogen (Biomedical Technologies, Inc).

    Article Title: Loss of Androgen Receptor Promotes Adipogensis but Suppresses Osteogenesis in Bone Marrow Stromal Cells
    Article Snippet: After incubation, the samples were put into DynaMag™-2 Magnet (Invitrogen) for 2 minutes and the bound beads were moved to each side. .. The confluent cultured BMSCs were lysed to extract mRNA, and 10 μg fresh mRNA used to determine the whole gene changes via mouse affymetrix microarray.

    Article Title: Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization
    Article Snippet: .. Pipettes 37 °C, 5% CO2 incubator (Thermo Fisher Scientific, Thermo Scientific™, model: Forma™ Series II 3110 Water-Jacketed) Cell culture microscope (Nikon Instruments, model: Eclipse TS100) Biosafety cabinet (The Baker, model: SterilGARD® e3) Standard tabletop centrifuge (Eppendorf, model: 5417 R) Invitrogen SDS-PAGE running cassette (Thermo Fisher Scientific, catalog number: ) Tank blot device (Bio-Rad Laboratories, model: Mini Trans-Blot® Module, catalog number: 1658029FC) ChemiDoc™ XRS+ Imager System (Bio-Rad Laboratories, model: ChemiDoc™ XRS+) Shaker (Fisher Scientific, model: Chemistry Mixer 346) Rocking platform (ARMA LAB, model: Orbital Shaker 100) Magnetic separation rack (Thermo Fisher Scientific, catalog number: 12321D) .. Image Lab 5.2.1 software ( )

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Briefly, Pellets from 250 ml of cultured C. albicans yeast cells were resuspended in 10 ml of lysis buffer (100 mM HEPES, pH 8.0, 20 mM magnesium acetate, 10% glycerol (v/v), 10 mM EGTA, 0.1 mM EDTA, 0.4% NP-40 supplemented with fresh protease inhibitors mixture (100 fold dilution; P8215 (Sigma-Aldrich) and 1 mM PMSF), and subsequently lysed by bead-beating for 3 x 2 minutes in a cold room. .. Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Transmission Assay:

    Article Title: Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
    Article Snippet: Paragraph title: Transmission electron microscopy (TEM) ... A DynaMag-2 magnet (Invitrogen, Carlsbad, CA) was used to separate the Dynabeads coated with CD42b linked to microparticles from the PPP.

    Sequencing:

    Article Title: Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
    Article Snippet: Paragraph title: Virome capture sequencing. ... The streptavidin capture beads complexed with biotinylated VirCapSeq-VERT probes were trapped (DynaMag-2 magnet; Thermo, Fisher) and washed once at 47°C and then twice more at room temperature with wash buffers of increasing stringency.

    Sonication:

    Article Title: Evaluation of Targeted Delivery to the Brain Using Magnetic Immunoliposomes and Magnetic Force
    Article Snippet: The mixture was placed under constant agitation at ambient temperature for 1 h and then extensively sonicated for 1.5 h to dissolve any aggregates and promote the formation of unilamellar liposomes. .. Excess lipid was removed by triple magnetic decantation using DynaMAG™-2 magnet (Invitrogen, Oxford, UK), followed by resuspension in HEPES buffer.

    Article Title: Molecular Dissection of Chromatin Maturation Via Click Chemistry
    Article Snippet: 750018, Life Technologies) 2× Tween Wash buffer (see recipe) Click Reaction Mixture (see recipe) Cytoplasm Wash Buffer (see recipe) DTT Wash Buffer (see recipe) Pull Down Sonication Buffer (see recipe) RIPA Buffer (see recipe) 5× Reducing sample buffer (Cat.No. .. 12321D, Life Technologies) QSonica, sonicator (Cat.No., QSonica) Cell lifter (Cat.No.

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: The lysates were then sonicated for 3 x 30 second pulses at 65% amplitude using a QSONICA 125W sonicator equipped with 1/8” probe. .. Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Affinity Purification:

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Paragraph title: Ydj1 affinity purification ... Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Binding Assay:

    Article Title: Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
    Article Snippet: Beads without capture antibody, as well as beads coated with IgG isotype control to correct for Fc binding, were also incubated with PPP as controls. .. A DynaMag-2 magnet (Invitrogen, Carlsbad, CA) was used to separate the Dynabeads coated with CD42b linked to microparticles from the PPP.

    Article Title: Molecular Dissection of Chromatin Maturation Via Click Chemistry
    Article Snippet: 12321D, Life Technologies) QSonica, sonicator (Cat.No., QSonica) Cell lifter (Cat.No. .. 3008, Corning) Low binding nuclease free 1.7mL tubes (Cat.No.

    Transmission Electron Microscopy:

    Article Title: Increased CD39 Nucleotidase Activity on Microparticles from Patients with Idiopathic Pulmonary Arterial Hypertension
    Article Snippet: Paragraph title: Transmission electron microscopy (TEM) ... A DynaMag-2 magnet (Invitrogen, Carlsbad, CA) was used to separate the Dynabeads coated with CD42b linked to microparticles from the PPP.

    Magnetic Beads:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: Twenty microliter of Pierce Protein A Magnetic Beads (Thermo Fisher Scientific) coupled with 10 μg anti-GFP polyclonal antibody (custom made) were added to each protein extract and incubated at 4°C for 1 h with rotation. .. The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification. .. The pellet was diluted in 40 μl lysis buffer and transferred into a 96 wells microplate (Greiner, Kremsmünster).

    Article Title: Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
    Article Snippet: The DNA plasmid template pUC19- ARS1 was randomly biotinylated and coupled to streptavidin-coated magnetic beads as described previously ( ). .. Supernatants of each step were removed by applying the reaction to a DynaMag-2 magnet (ThermoFisher Scientific) to isolate the DNA coupled to magnetic streptavidin beads from the supernatant.

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Freshly washed anti-FLAG M2 magnetic beads (30 μl slurry per sample) were added and the samples were incubated with end-over-end rotation for 3 hours at 4°C. .. Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Isolation:

    Article Title: Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis
    Article Snippet: Paragraph title: BMEC isolation ... Dynabead-CD31 capture, BMEC isolations, and washes were done using a DynaMag-2 magnet (Life Technologies).

    Article Title: Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
    Article Snippet: Paragraph title: Isolation of primary microvascular ECs ... The EC-bound beads were captured using the DynaMag™-2 Magnet (Thermo Fisher Scientific) and washed three times with PBS.

    Article Title: Loss of Androgen Receptor Promotes Adipogensis but Suppresses Osteogenesis in Bone Marrow Stromal Cells
    Article Snippet: The hematopoietic lineages cells were depleted with the magnetic bead isolation protocol as we previously described ( ). .. After incubation, the samples were put into DynaMag™-2 Magnet (Invitrogen) for 2 minutes and the bound beads were moved to each side.

    Co-Immunoprecipitation Assay:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: Paragraph title: Co-immunoprecipitation assay and western blot analysis ... The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100).

    Microscopy:

    Article Title: Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization
    Article Snippet: .. Pipettes 37 °C, 5% CO2 incubator (Thermo Fisher Scientific, Thermo Scientific™, model: Forma™ Series II 3110 Water-Jacketed) Cell culture microscope (Nikon Instruments, model: Eclipse TS100) Biosafety cabinet (The Baker, model: SterilGARD® e3) Standard tabletop centrifuge (Eppendorf, model: 5417 R) Invitrogen SDS-PAGE running cassette (Thermo Fisher Scientific, catalog number: ) Tank blot device (Bio-Rad Laboratories, model: Mini Trans-Blot® Module, catalog number: 1658029FC) ChemiDoc™ XRS+ Imager System (Bio-Rad Laboratories, model: ChemiDoc™ XRS+) Shaker (Fisher Scientific, model: Chemistry Mixer 346) Rocking platform (ARMA LAB, model: Orbital Shaker 100) Magnetic separation rack (Thermo Fisher Scientific, catalog number: 12321D) .. Image Lab 5.2.1 software ( )

    Purification:

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification. .. The pellet was diluted in 40 μl lysis buffer and transferred into a 96 wells microplate (Greiner, Kremsmünster).

    Article Title: Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
    Article Snippet: The streptavidin capture beads complexed with biotinylated VirCapSeq-VERT probes were trapped (DynaMag-2 magnet; Thermo, Fisher) and washed once at 47°C and then twice more at room temperature with wash buffers of increasing stringency. .. The PCR products were purified (Agencourt Ampure DNA purification beads; Beckman Coulter, Brea, CA, USA) and quantitated by Bioanalyzer (Agilent) for Illumina sequencing.

    Polymerase Chain Reaction:

    Article Title: Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
    Article Snippet: The VirCap probe library (4.5 μl) was added and hybridized at 47°C for 12 h in a standard PCR thermocycler. .. The streptavidin capture beads complexed with biotinylated VirCapSeq-VERT probes were trapped (DynaMag-2 magnet; Thermo, Fisher) and washed once at 47°C and then twice more at room temperature with wash buffers of increasing stringency.

    Gel Extraction:

    Article Title: The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
    Article Snippet: Non-stick RNase-free 0.5 ml microfuge tubes (Invitrogen, cat. no. AM12350) for optional rapid gel extraction Microfuge tube spin filter (VWR, cat. no. 29442-752) for optional rapid gel extraction. .. Thermal cycler (Bio-Rad, cat. no. 170-9713) DynaMag-2 separation rack (Invitrogen, cat. no. 12321D) ThermoMixer (VWR, cat. no. 21516-170) 2100 BioAnalyzer (Agilent Technologies, cat. no. G2940CA) Genome Analyzer II or HiSeq 2000 (Illumina) Computer hardware – a 64-bit computer running Linux with at least 4 GB of RAM for the manual analysis options.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Molecular Dissection of Chromatin Maturation Via Click Chemistry
    Article Snippet: .. 12321D, Life Technologies) QSonica, sonicator (Cat.No., QSonica) Cell lifter (Cat.No. .. 3008, Corning) Low binding nuclease free 1.7mL tubes (Cat.No.

    SDS Page:

    Article Title: The Arabidopsis B-BOX Protein BZS1/BBX20 Interacts with HY5 and Mediates Strigolactone Regulation of Photomorphogenesis
    Article Snippet: The beads were then collected by DynaMag™ -2 Magnet (Thermo Fisher Scientific) and washed three times with wash buffer (20 mM HEPES, pH7.5, 40 mM KCl, 0.1% Triton X-100). .. The bonded proteins were eluted with 50 μL 2 × SDS loading buffer by incubating at 95°C for 10 min. For western blot analysis, proteins were separated by SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane (Merck Millipore Corporation, Germany) by semi-dry transfer cell (Bio-Rad Laboratories, USA).

    Article Title: Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization
    Article Snippet: .. Pipettes 37 °C, 5% CO2 incubator (Thermo Fisher Scientific, Thermo Scientific™, model: Forma™ Series II 3110 Water-Jacketed) Cell culture microscope (Nikon Instruments, model: Eclipse TS100) Biosafety cabinet (The Baker, model: SterilGARD® e3) Standard tabletop centrifuge (Eppendorf, model: 5417 R) Invitrogen SDS-PAGE running cassette (Thermo Fisher Scientific, catalog number: ) Tank blot device (Bio-Rad Laboratories, model: Mini Trans-Blot® Module, catalog number: 1658029FC) ChemiDoc™ XRS+ Imager System (Bio-Rad Laboratories, model: ChemiDoc™ XRS+) Shaker (Fisher Scientific, model: Chemistry Mixer 346) Rocking platform (ARMA LAB, model: Orbital Shaker 100) Magnetic separation rack (Thermo Fisher Scientific, catalog number: 12321D) .. Image Lab 5.2.1 software ( )

    Plasmid Preparation:

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: Paragraph title: Detection of ternary protein complexes using a HaloTag vector ... Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification.

    Article Title: Mcm10 regulates DNA replication elongation by stimulating the CMG replicative helicase
    Article Snippet: The DNA plasmid template pUC19- ARS1 was randomly biotinylated and coupled to streptavidin-coated magnetic beads as described previously ( ). .. Supernatants of each step were removed by applying the reaction to a DynaMag-2 magnet (ThermoFisher Scientific) to isolate the DNA coupled to magnetic streptavidin beads from the supernatant.

    Software:

    Article Title: The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments
    Article Snippet: Thermal cycler (Bio-Rad, cat. no. 170-9713) DynaMag-2 separation rack (Invitrogen, cat. no. 12321D) ThermoMixer (VWR, cat. no. 21516-170) 2100 BioAnalyzer (Agilent Technologies, cat. no. G2940CA) Genome Analyzer II or HiSeq 2000 (Illumina) Computer hardware – a 64-bit computer running Linux with at least 4 GB of RAM for the manual analysis options. .. FastX-toolkit ( ) and Illumina quality filter software ( ) installed locally for the manual analysis options, or through Galaxy for the Galaxy analysis option.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Lung-Derived Factors Mediate Breast Cancer Cell Migration through CD44 Receptor-Ligand Interactions in a Novel Ex Vivo System for Analysis of Organ-Specific Soluble Proteins 1 System for Analysis of Organ-Specific Soluble Proteins 1 2
    Article Snippet: .. Resulting bead-antibody-antigen complexes were removed using a DynaMag-2 magnet (Invitrogen), and depleted media were assessed by Quantikine ELISA kits specific for mouse SELL or OPN (R & D Systems). ..

    Trypan Blue Exclusion Assay:

    Article Title: Peptide-functionalized quantum dots for potential applications in the imaging and treatment of obesity
    Article Snippet: The EC-bound beads were captured using the DynaMag™-2 Magnet (Thermo Fisher Scientific) and washed three times with PBS. .. The viable EC cell density was determined using Trypan Blue exclusion assay on the Countess® Automated Cell Counter (Thermo Fisher Scientific).

    Immunoprecipitation:

    Article Title: Macrophage-derived lipocalin-2 transports iron in the tumor microenvironment
    Article Snippet: Paragraph title: LCN-2 immunoprecipitation ... Beads were precipitated using the DynaMag-2 magnet (Thermo Fisher) and washed three times with IP buffer.

    DNA Purification:

    Article Title: Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
    Article Snippet: The streptavidin capture beads complexed with biotinylated VirCapSeq-VERT probes were trapped (DynaMag-2 magnet; Thermo, Fisher) and washed once at 47°C and then twice more at room temperature with wash buffers of increasing stringency. .. The PCR products were purified (Agencourt Ampure DNA purification beads; Beckman Coulter, Brea, CA, USA) and quantitated by Bioanalyzer (Agilent) for Illumina sequencing.

    Lysis:

    Article Title: The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome
    Article Snippet: The third day, culture medium was discarded, cells were washed once with 2 ml Phosphate Buffer Saline without calcium and magnesium (PBS), incubated for 5 to 10 min in 900 μl of lysis buffer, and scraped. .. Magnetic beads were purified on a DynaMag™-2 magnet (Thermo Fisher Scientific, Waltham), and submitted to 6 cycles of wash with 900ml PBS containing 0.1% Tween followed by magnet purification.

    Article Title: Ydj1 governs fungal morphogenesis and stress response, and facilitates mitochondrial protein import via Mas1 and Mas2
    Article Snippet: Briefly, Pellets from 250 ml of cultured C. albicans yeast cells were resuspended in 10 ml of lysis buffer (100 mM HEPES, pH 8.0, 20 mM magnesium acetate, 10% glycerol (v/v), 10 mM EGTA, 0.1 mM EDTA, 0.4% NP-40 supplemented with fresh protease inhibitors mixture (100 fold dilution; P8215 (Sigma-Aldrich) and 1 mM PMSF), and subsequently lysed by bead-beating for 3 x 2 minutes in a cold room. .. Using a DynaMag-2 magnet (ThermoFisher), the beads were collected on the side of the sample tube and the supernatant was discarded.

    Hood:

    Article Title: Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization
    Article Snippet: .. Pipettes 37 °C, 5% CO2 incubator (Thermo Fisher Scientific, Thermo Scientific™, model: Forma™ Series II 3110 Water-Jacketed) Cell culture microscope (Nikon Instruments, model: Eclipse TS100) Biosafety cabinet (The Baker, model: SterilGARD® e3) Standard tabletop centrifuge (Eppendorf, model: 5417 R) Invitrogen SDS-PAGE running cassette (Thermo Fisher Scientific, catalog number: ) Tank blot device (Bio-Rad Laboratories, model: Mini Trans-Blot® Module, catalog number: 1658029FC) ChemiDoc™ XRS+ Imager System (Bio-Rad Laboratories, model: ChemiDoc™ XRS+) Shaker (Fisher Scientific, model: Chemistry Mixer 346) Rocking platform (ARMA LAB, model: Orbital Shaker 100) Magnetic separation rack (Thermo Fisher Scientific, catalog number: 12321D) .. Image Lab 5.2.1 software ( )

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  • 90
    Thermo Fisher dynamag 2 magnet
    Dynamag 2 Magnet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynamag 2 magnet/product/Thermo Fisher
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    dynamag 2 magnet - by Bioz Stars, 2020-01
    90/100 stars
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    The SampleRack is the tube holder rack for the DynaMag 2 magnet This removable plastic rack also comes with the DynaMag 2 magnet and can now be purchased as a
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