dynabeads kilobase binder kit  (Thermo Fisher)


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    Name:
    Dynabeads kilobaseBINDER Kit
    Description:
    Streptavidin coupled Dynabeads is the gold standard for isolation and handling of biotinylated nucleic acids antibodies or other biotinylated ligands and targets The very high binding affinity of the streptavidin biotin interaction Kd 10 15 is utilized in a vast number of applications The amount of biotinylated DNA immobilized will depend on fragment size Due to steric hindrance the binding efficiency is significantly reduced when the fragment size exceeds 2 kb The specially designed Binding Solution supplied in the Dynabeads kilobaseBINDER Kit contains a patented immobilization activator that significantly increases the binding capacity for large double stranded DNA fragments Applications The Dynabeads kilobaseBINDERTM Kit is designed for immobilizing long double stranded DNA molecules 2 kb This product has been useful for innovative applications in cell biology e g building chromatin beads that mimic chromosomes for the study of self organization of microtubules into bipolar spindles building synthetic nuclei for studying RNA export from the nucleus in vitro reconstituting nuclear movement along microtubules and examination of the molecular basis of this movement in vitro A reference list is available upon request Benefits and Features • Increased binding capacity for 2 kb dsDNA molecules• Chromatin isolation within minutes• High batch to batch reproducibility securing consistent results in your applicationBinding capacity The size of the molecule and the biotinylation procedure will affect the binding capacity The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules The Dynabeads are coated with a monolayer of recombinant streptavidin This leaves the majority of the biotin binding sites sterically available for binding not only of free biotin but also biotinylated ligands targets The streptavidin monolayer is covalently coupled to the beads ensuring negligible leakage and superior reproducibility With the unique Binding Solution supplied in this kit it is possible to immobilize 70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads The Invitrogen Dynal quality control tests ensure the following binding capacity criteria to be fulfilled • 10 times better capacity for binding of a biotinylated 4 kb DNA fragments per mg beads when using the Binding Solution compared to no use of Binding Solution • 195 μg 6 pmol biotinylated 50 kb DNA fragment per mg beads In addition the EMBL Heidelberg group who developed the Binding Solution has reported the following results • Approximately 400 μg 100 pmole biotinylated 6 2 kb DNA fragments per mg Dynabeads M 280 Streptavidin The product holds reputable Dynal high standards with respect to reproducibility both within ad between batches and drives reliability for your results
    Catalog Number:
    60101
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|Sequencing Reaction Purification
    Category:
    Beads Microspheres
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    Structured Review

    Thermo Fisher dynabeads kilobase binder kit
    Streptavidin coupled Dynabeads is the gold standard for isolation and handling of biotinylated nucleic acids antibodies or other biotinylated ligands and targets The very high binding affinity of the streptavidin biotin interaction Kd 10 15 is utilized in a vast number of applications The amount of biotinylated DNA immobilized will depend on fragment size Due to steric hindrance the binding efficiency is significantly reduced when the fragment size exceeds 2 kb The specially designed Binding Solution supplied in the Dynabeads kilobaseBINDER Kit contains a patented immobilization activator that significantly increases the binding capacity for large double stranded DNA fragments Applications The Dynabeads kilobaseBINDERTM Kit is designed for immobilizing long double stranded DNA molecules 2 kb This product has been useful for innovative applications in cell biology e g building chromatin beads that mimic chromosomes for the study of self organization of microtubules into bipolar spindles building synthetic nuclei for studying RNA export from the nucleus in vitro reconstituting nuclear movement along microtubules and examination of the molecular basis of this movement in vitro A reference list is available upon request Benefits and Features • Increased binding capacity for 2 kb dsDNA molecules• Chromatin isolation within minutes• High batch to batch reproducibility securing consistent results in your applicationBinding capacity The size of the molecule and the biotinylation procedure will affect the binding capacity The capacity also depends on steric availability and charge interaction between bead and molecule and between molecules The Dynabeads are coated with a monolayer of recombinant streptavidin This leaves the majority of the biotin binding sites sterically available for binding not only of free biotin but also biotinylated ligands targets The streptavidin monolayer is covalently coupled to the beads ensuring negligible leakage and superior reproducibility With the unique Binding Solution supplied in this kit it is possible to immobilize 70 pmoles of a 4 kb biotinylated DNA fragment and 80 pmoles of a 10 kb fragment per mg beads The Invitrogen Dynal quality control tests ensure the following binding capacity criteria to be fulfilled • 10 times better capacity for binding of a biotinylated 4 kb DNA fragments per mg beads when using the Binding Solution compared to no use of Binding Solution • 195 μg 6 pmol biotinylated 50 kb DNA fragment per mg beads In addition the EMBL Heidelberg group who developed the Binding Solution has reported the following results • Approximately 400 μg 100 pmole biotinylated 6 2 kb DNA fragments per mg Dynabeads M 280 Streptavidin The product holds reputable Dynal high standards with respect to reproducibility both within ad between batches and drives reliability for your results
    https://www.bioz.com/result/dynabeads kilobase binder kit/product/Thermo Fisher
    Average 98 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dynabeads kilobase binder kit - by Bioz Stars, 2020-09
    98/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: Geminin Inhibits a Late Step in the Formation of Human Pre-replicative Complexes *
    Article Snippet: .. The immobilization of biotinylated DNA to magnetic beads was carried out using the Dynabeads kilobaseBINDER kit (Invitrogen) according to the manufacturer's instruction. ..

    Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
    Article Snippet: .. 50 ng of DNA) were annealed to 20 pmol of a biotinylated microsatellite oligonucleotide [GATGATCCGACGCAT(CA)12 , GATGATCCGACGCAT(CT)12 , (CAA)12 , (CTT)12 , (CAT)12 , (CTA)12 , or (TAA)12 ] by denaturing at 95°C for 10 min and annealing at 60°C for 1 min; the hybrids were then bound to 100 μg of streptavidin-coated magnetic beads (Dynabeads KilobaseBinder kit; Dynal). .. Following incubation at room temperature for 3 h the Dynabead-DNA complexes were washed twice (10 mM Tris-Cl, pH 7.5; 1 mM EDTA; 2.0 M NaCl) and resuspended in 50 μl of TE buffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA; pH 8.0).

    Article Title: Strand-Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Measurement of Arenavirus Genomic and Antigenomic RNAs
    Article Snippet: .. Affinity Purification of Biotinylated cDNAs Biotinylated cDNAs were purified using streptavidin-coated magnetic beads (11205D, Dynabeads M-280 Streptavidin, Life Technologies) and the Dynabeads kilobaseBINDER Kit (60101, Life Technologies). .. For each RT reaction, a volume of 10 μl of magnetic beads was pre-equilibrated in 40 μl of Binding Solution.

    Isolation:

    Article Title: Mechanisms of precise genome editing using oligonucleotide donors
    Article Snippet: .. The biotinylated DNA fragments were isolated using a Dynabeads Kilobase BINDER kit (Life Technologies) according to the manufacturer's protocol, except that all reagents were supplemented with 0.1% BSA, and two additional washes using 0.1 M NaOH and 0.05 M NaCl at room temperature and one more rinse with 95°C water were performed to remove the noncovalently linked genomic fragments. .. The biotinylated genomic fragments were detected with a 40-cycle PCR using Phusion Hot Start II High-Fidelity DNA polymerase (Thermo Scientific).

    Laser Capture Microdissection:

    Article Title: Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling
    Article Snippet: .. The resulting PCR product from each replicate LAM-PCR reaction was purified on 8 µl of magnetic Dynabeads M280 (Dynabeads kilobaseBINDER Kit, Thermo Fisher Scientific Cat. # 60101). .. The beads were prewashed three times with 100 µl PBS and once with 20 µl of the Binding Solution included in the kit, using a multi-well magnet.

    Purification:

    Article Title: Strand-Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Measurement of Arenavirus Genomic and Antigenomic RNAs
    Article Snippet: .. Affinity Purification of Biotinylated cDNAs Biotinylated cDNAs were purified using streptavidin-coated magnetic beads (11205D, Dynabeads M-280 Streptavidin, Life Technologies) and the Dynabeads kilobaseBINDER Kit (60101, Life Technologies). .. For each RT reaction, a volume of 10 μl of magnetic beads was pre-equilibrated in 40 μl of Binding Solution.

    Article Title: Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling
    Article Snippet: .. The resulting PCR product from each replicate LAM-PCR reaction was purified on 8 µl of magnetic Dynabeads M280 (Dynabeads kilobaseBINDER Kit, Thermo Fisher Scientific Cat. # 60101). .. The beads were prewashed three times with 100 µl PBS and once with 20 µl of the Binding Solution included in the kit, using a multi-well magnet.

    Polymerase Chain Reaction:

    Article Title: Engineering Gene Networks to Emulate Drosophila Embryonic Pattern FormationEngineering Gene Networks to Probe Embryonic Pattern Formation in Flies
    Article Snippet: .. Gene network reactions Paramagnetic beads were coated with PCR DNA (with one primer biotinylated) using a Dynabeads Kilobase Binder Kit (Dynal, Oslo, Norway; #601.01). ..

    Article Title: Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling
    Article Snippet: .. The resulting PCR product from each replicate LAM-PCR reaction was purified on 8 µl of magnetic Dynabeads M280 (Dynabeads kilobaseBINDER Kit, Thermo Fisher Scientific Cat. # 60101). .. The beads were prewashed three times with 100 µl PBS and once with 20 µl of the Binding Solution included in the kit, using a multi-well magnet.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
    Article Snippet: .. 50 ng of DNA) were annealed to 20 pmol of a biotinylated microsatellite oligonucleotide [GATGATCCGACGCAT(CA)12 , GATGATCCGACGCAT(CT)12 , (CAA)12 , (CTT)12 , (CAT)12 , (CTA)12 , or (TAA)12 ] by denaturing at 95°C for 10 min and annealing at 60°C for 1 min; the hybrids were then bound to 100 μg of streptavidin-coated magnetic beads (Dynabeads KilobaseBinder kit; Dynal). .. Following incubation at room temperature for 3 h the Dynabead-DNA complexes were washed twice (10 mM Tris-Cl, pH 7.5; 1 mM EDTA; 2.0 M NaCl) and resuspended in 50 μl of TE buffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA; pH 8.0).

    Cellular Antioxidant Activity Assay:

    Article Title: Isolation and Characterization of Polymorphic DNA from Entamoeba histolytica
    Article Snippet: .. 50 ng of DNA) were annealed to 20 pmol of a biotinylated microsatellite oligonucleotide [GATGATCCGACGCAT(CA)12 , GATGATCCGACGCAT(CT)12 , (CAA)12 , (CTT)12 , (CAT)12 , (CTA)12 , or (TAA)12 ] by denaturing at 95°C for 10 min and annealing at 60°C for 1 min; the hybrids were then bound to 100 μg of streptavidin-coated magnetic beads (Dynabeads KilobaseBinder kit; Dynal). .. Following incubation at room temperature for 3 h the Dynabead-DNA complexes were washed twice (10 mM Tris-Cl, pH 7.5; 1 mM EDTA; 2.0 M NaCl) and resuspended in 50 μl of TE buffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA; pH 8.0).

    Affinity Purification:

    Article Title: Strand-Specific Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Measurement of Arenavirus Genomic and Antigenomic RNAs
    Article Snippet: .. Affinity Purification of Biotinylated cDNAs Biotinylated cDNAs were purified using streptavidin-coated magnetic beads (11205D, Dynabeads M-280 Streptavidin, Life Technologies) and the Dynabeads kilobaseBINDER Kit (60101, Life Technologies). .. For each RT reaction, a volume of 10 μl of magnetic beads was pre-equilibrated in 40 μl of Binding Solution.

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  • 98
    Thermo Fisher dynabeads kilobase binder kit
    Dynabeads Kilobase Binder Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads kilobase binder kit/product/Thermo Fisher
    Average 98 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dynabeads kilobase binder kit - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

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