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GE Healthcare dyenamic et dye terminator sequencing kit
Dyenamic Et Dye Terminator Sequencing Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 14 article reviews
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dyenamic et dye terminator sequencing kit - by Bioz Stars, 2020-02
85/100 stars

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Clone Assay:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: Briefly, PCR products were purified with the QIAquick PCR purification kit (QIAGEN Sciences), and cloned into pGEM-T vector (Promega, Madison, WI, USA). .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA). .. A total of 230 cDNA clones were sequenced, 27 clones obtained from the reverse library (downregulated genes) and 30 clones obtained from the upregulated genes library.

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: Paragraph title: Cloning the S100A7 gene ... The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Amplification:

Article Title: Concordance between Phylogeographical and Biogeographical Patterns in the Brazilian Cerrado: Diversification of the Endemic Tree Dalbergia miscolobium (Fabaceae)
Article Snippet: Paragraph title: Population sampling and DNA amplification ... Thermal conditions of the reaction were as follows: initial denaturation at 94°C for 2 min, followed by 35 cycles at 94°C for 1 min, 56°C (cpDNA) or 60°C (nrDNA) for 1 min, and 72°C for 1 min, and a final elongation at 72°C for 7 min. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing Kit (GE Healthcare, UK).

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: The DNA fragments of the envelope gene regions of 9 DENV-1, 40 DENV-2, and 18 DENV-3 isolates were amplified by PCR using 5 μl of cDNA in a 50-μl reaction mixture containing 0.3 mM deoxynucleoside triphosphates, 2.5 U AmpliTaq DNA polymerase (Applied Biosystems), 1× PCR buffer, 1.5 mM MgCl2 , and 15 pmol of each forward and reverse primer. .. Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare).

Article Title: Phylogeography of the endangered rosewood Dalbergia nigra (Fabaceae): insights into the evolutionary history and conservation of the Brazilian Atlantic Forest
Article Snippet: Paragraph title: DNA amplification and sequencing ... Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing kit (GE Healthcare, Buckinghamshire, England, UK).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Three SAGE libraries were generated using two larynx cancer samples (one with lymph node metastasis or N+ and one with no lymph node metastasis or N0) and a normal control library pooled from two normal samples (surgical margins from one N+ and one N0 larynx cancer).

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: The DNA fragments of the envelope gene region of 97 DENV-1, 23 DENV-2, 5 DENV-3, and 74 DENV-4 were amplified by PCR using 5 µl of cDNA in a 50 µl reaction mixture containing 0.3 mM dNTPs, 2.5 U AmpliTaq DNA polymerase (Applied Biosystems), 1× PCR buffer, 1.5 mM MgCl2 and 15 pmol of each forward and reverse primer. .. Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: Bacterial colonies were selected randomly and PCR amplified, using M13 forward and reverse primers. .. Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences).

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: The DNA fragments of the E gene and the overlapping DNA fragments covering the entire DENV-1 genome were amplified using AmpliTaq DNA polymerase (Applied Biosystems) and purified using the QIAquick PCR purification kit and QIAquick gel extraction kit (Qiagen). .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions.

Polymerase Chain Reaction:

Article Title: Concordance between Phylogeographical and Biogeographical Patterns in the Brazilian Cerrado: Diversification of the Endemic Tree Dalbergia miscolobium (Fabaceae)
Article Snippet: .. Thermal conditions of the reaction were as follows: initial denaturation at 94°C for 2 min, followed by 35 cycles at 94°C for 1 min, 56°C (cpDNA) or 60°C (nrDNA) for 1 min, and 72°C for 1 min, and a final elongation at 72°C for 7 min. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing Kit (GE Healthcare, UK). .. Sequencing reactions were analyzed on a MegaBACE 1000 automated sequencer (GE Healthcare) following the manufacturer's instructions.

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions. .. Results We analyzed sequences of CDR3 by PCR from the lymph node biopsy specimen, by which the diagnosis of DLBCL was made, and from the bone marrow sample, in which lymphoplasmacytic cell proliferation was observed.

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: Bacterial colonies were analyzed using PCR and the M13 forward and M13 reverse primers to identify those with an insert. .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare). .. This indicated that the insertion of the 306 bp fragment corresponding to the S100A7 gene into the expression vector pAE was correct.

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: The PCR-amplified DNA fragments were purified using QIAquick PCR purification kits (Qiagen) according to the manufacturer's instructions. .. Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: .. Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare). ..

Article Title: Phylogeography of the endangered rosewood Dalbergia nigra (Fabaceae): insights into the evolutionary history and conservation of the Brazilian Atlantic Forest
Article Snippet: .. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing kit (GE Healthcare, Buckinghamshire, England, UK). .. Sequencing reactions were analysed on a MegaBACE 1000 automated sequencer (GE Healthcare).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: An aliquot of each well was then used directly in a PCR reaction, with forward and reverse M13 primers. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: The PCR-amplified DNA fragments were purified using QIAquick PCR purification kits (QIAGEN) according to the manufacturer's instructions. .. Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: Bacterial colonies were selected randomly and PCR amplified, using M13 forward and reverse primers. .. Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences).

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: The DNA fragments of the E gene and the overlapping DNA fragments covering the entire DENV-1 genome were amplified using AmpliTaq DNA polymerase (Applied Biosystems) and purified using the QIAquick PCR purification kit and QIAquick gel extraction kit (Qiagen). .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions.

Construct:

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: The constructs were introduced into TOP10 competent cells. .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: The construct was introduced into competent E. coli DH5α cells with the Subcloning Efficiency™ Chemically Competent E. coli kit (Invitrogen). .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare).

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: The constructs were introduced into the DH10-B competent cells. .. Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences).

Random Hexamer Labeling:

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: Viral RNA extracted by the QIAamp viral RNA minikit was converted to cDNA using the Transcriptor high-fidelity kit (Roche) and random hexamer oligonucleotides according to the manufacturer's instructions. .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions.

Expressing:

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: The S100A7 fragment was removed from the pCR2.1-TOPO vector by digestion with the restriction enzymes Xho I and Hind III and attached to the expression vector pAE, which contained the 6xHis-tag peptide in the N-terminal region [ ], according to the protocol for the enzyme T4 DNA ligase (Promega). .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. The raw data files are available at the Gene Expression Omnibus database (GEO) under the accession numbers: GSM303325 (pool of normal samples); GSM303340 (N0 tumor), GSM303349 (N+ tumor).

Sampling:

Article Title: Concordance between Phylogeographical and Biogeographical Patterns in the Brazilian Cerrado: Diversification of the Endemic Tree Dalbergia miscolobium (Fabaceae)
Article Snippet: Paragraph title: Population sampling and DNA amplification ... Thermal conditions of the reaction were as follows: initial denaturation at 94°C for 2 min, followed by 35 cycles at 94°C for 1 min, 56°C (cpDNA) or 60°C (nrDNA) for 1 min, and 72°C for 1 min, and a final elongation at 72°C for 7 min. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing Kit (GE Healthcare, UK).

Hybridization:

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: Paragraph title: Rapid Subtractive Hybridization (RaSH ) ... The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: Paragraph title: Rapid Subtractive Hybridization ... Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences).

Ligation:

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Ligation reactions were used to transform One Shot® TOP10 Eletrocomp™ E. coli cells using 0.2 cm cuvettes and a Gene Pulser II electroporator (Bio-Rad Laboratories, Hercules, CA, USA) set at 2.5 kV, 25 mF and 200 Ω. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Generated:

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare). .. As a complement to data generated in this study, we added sequences previously obtained by for the ND5 gene, which include 15 L. colocola and 21 L. tigrinus individuals ( , , , , – ).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Three SAGE libraries were generated using two larynx cancer samples (one with lymph node metastasis or N+ and one with no lymph node metastasis or N0) and a normal control library pooled from two normal samples (surgical margins from one N+ and one N0 larynx cancer).

DNA Sequencing:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: Paragraph title: Subcloning of polymerase chain reaction products and DNA sequencing ... Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Sequencing:

Article Title: Concordance between Phylogeographical and Biogeographical Patterns in the Brazilian Cerrado: Diversification of the Endemic Tree Dalbergia miscolobium (Fabaceae)
Article Snippet: .. Thermal conditions of the reaction were as follows: initial denaturation at 94°C for 2 min, followed by 35 cycles at 94°C for 1 min, 56°C (cpDNA) or 60°C (nrDNA) for 1 min, and 72°C for 1 min, and a final elongation at 72°C for 7 min. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing Kit (GE Healthcare, UK). .. Sequencing reactions were analyzed on a MegaBACE 1000 automated sequencer (GE Healthcare) following the manufacturer's instructions.

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions. .. Results We analyzed sequences of CDR3 by PCR from the lymph node biopsy specimen, by which the diagnosis of DLBCL was made, and from the bone marrow sample, in which lymphoplasmacytic cell proliferation was observed.

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA). .. A total of 230 cDNA clones were sequenced, 27 clones obtained from the reverse library (downregulated genes) and 30 clones obtained from the upregulated genes library.

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare). .. This indicated that the insertion of the 306 bp fragment corresponding to the S100A7 gene into the expression vector pAE was correct.

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: .. Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions. ..

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: .. Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare). ..

Article Title: Phylogeography of the endangered rosewood Dalbergia nigra (Fabaceae): insights into the evolutionary history and conservation of the Brazilian Atlantic Forest
Article Snippet: .. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing kit (GE Healthcare, Buckinghamshire, England, UK). .. Sequencing reactions were analysed on a MegaBACE 1000 automated sequencer (GE Healthcare).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). .. Three SAGE libraries were generated using two larynx cancer samples (one with lymph node metastasis or N+ and one with no lymph node metastasis or N0) and a normal control library pooled from two normal samples (surgical margins from one N+ and one N0 larynx cancer).

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: .. Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences). .. The sequences were analyzed, using an annotation pipeline that consists of four steps: 1) quality checking, phred base-calling, cutoff 0.05 [ ]; 2) vector trimming and removal of undesirable sequences such as bacterial, mitochondrial, and rRNA sequences; 3) masking of repetitive elements and screening of low-complexity regions by Repeat Masker, using the default settings [ ]; and 4) annotation against existing databases, using BLASTN with default parameters.

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions. ..

DNA Extraction:

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare).

Isolation:

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: Paragraph title: Viral isolation and sequencing. ... Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: Sequencing of all PCR-positive, isolation-positive samples was attempted but insufficient RNA and isolation failures resulted in some PCR positive cohort and cluster samples to be excluded in this study. .. Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Subcloning:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: Paragraph title: Subcloning of polymerase chain reaction products and DNA sequencing ... Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: The construct was introduced into competent E. coli DH5α cells with the Subcloning Efficiency™ Chemically Competent E. coli kit (Invitrogen). .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare).

Size-exclusion Chromatography:

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: PCR reactions for DENV-1, -3, and 4 were subjected to 1 cycle of 95°C for 5 min, 35 cycles of 94°C for 30 sec, 50°C for 1 min, 72°C for 2 min, and 1 cycle of 72°C for 15 min. PCR reactions for DENV-2 were subjected to the same thermal conditions as the others, except the annealing temperature was changed to 55°C. .. Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Purification:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: After bacterial transformation, plasmids were purified and subjected to sequence determination. .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: Aliquots (10 µg) of the tester PCR products (penile carcinoma or normal tissue) were digested with 20 units of XhoI (Invitrogen Life Technologies, California, USA) and purified with phenol-chloroform extraction and ethanol precipitation. .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: Purified DNA fragments were used for sequencing. .. Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: For these three segments, the PCR conditions were identical and began with one step of 94°C for 3 min, 5 cycles (Touchdown) of 94°C for 45 s, 55-51°C for 45 s, 72°C for 1 min 30 s followed by 40 cycles of 94°C for 45 s, 50°C for 30 s, 72°C for 1 min 30 s and final extension of 72°C for 30 min. Thermocycling conditions for the ATP8 gene consisted of an initial denaturing step at 94°C for 3 min followed by 35 cycles of 94°C for 45 s, 50°C for 45 s, 72°C for 1 min 30 s and final extension of 72°C for 10 min. PCR products were visualized on a 1% agarose gel stained with GelRed® (Biotium Inc.) and then purified using a protocol based on precipitation with ammonium acetate and isopropanol, or the enzymatic method with the enzymes Exonuclease I (EXO I) and Shrimp Alkaline Phosphatase (SAP). .. Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare).

Article Title: Phylogeography of the endangered rosewood Dalbergia nigra (Fabaceae): insights into the evolutionary history and conservation of the Brazilian Atlantic Forest
Article Snippet: Following the PCR, the products were viewed on 1% TAE agarose gels and purified by 20% PEG (polyethylene glycol) precipitation. .. Each PCR product was then double-strand sequenced using the DYEnamic ET dye terminator sequencing kit (GE Healthcare, Buckinghamshire, England, UK).

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: Purified DNA fragments were used for sequencing. .. Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions. ..

Electroporation Bacterial Transformation:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: After bacterial transformation, plasmids were purified and subjected to sequence determination. .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Polyacrylamide Gel Electrophoresis:

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Staining:

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: For these three segments, the PCR conditions were identical and began with one step of 94°C for 3 min, 5 cycles (Touchdown) of 94°C for 45 s, 55-51°C for 45 s, 72°C for 1 min 30 s followed by 40 cycles of 94°C for 45 s, 50°C for 30 s, 72°C for 1 min 30 s and final extension of 72°C for 30 min. Thermocycling conditions for the ATP8 gene consisted of an initial denaturing step at 94°C for 3 min followed by 35 cycles of 94°C for 45 s, 50°C for 45 s, 72°C for 1 min 30 s and final extension of 72°C for 10 min. PCR products were visualized on a 1% agarose gel stained with GelRed® (Biotium Inc.) and then purified using a protocol based on precipitation with ammonium acetate and isopropanol, or the enzymatic method with the enzymes Exonuclease I (EXO I) and Shrimp Alkaline Phosphatase (SAP). .. Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare).

Plasmid Preparation:

Article Title: Development of diffuse large B-cell lymphoma in a patient with Waldenstr?m's macroglobulinemia/lymphoplasmacytic lymphoma: clonal identity between two B-cell neoplasms
Article Snippet: Briefly, PCR products were purified with the QIAquick PCR purification kit (QIAGEN Sciences), and cloned into pGEM-T vector (Promega, Madison, WI, USA). .. Both strands of each PCR product were sequenced using DYEnamic ET Dye Terminator sequencing kit (Amersham, Buckinghamshire, UK) and the MegaBase sequence system (Amersham) according to the manufacturer's instructions.

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: The fragments were inserted into XhoI-digested pZERO plasmid (1 µg/µl) at 16°C for three hours. .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Expression of human protein S100A7 (psoriasin), preparation of antibody and application to human larynx squamous cell carcinoma
Article Snippet: .. The transformants were selected by PCR and plasmid DNA was extracted as described by Sambrook and Russell [ ] and sequenced using the DYEnamic ET Dye Terminator Sequencing kit (GE Healthcare) with the automatic sequencer MegaBace™ 1000 (GE Healthcare). .. This indicated that the insertion of the 306 bp fragment corresponding to the S100A7 gene into the expression vector pAE was correct.

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Concatamers were submitted to polyacrylamide gel electrophoresis and regions ranging from 300–500 bp, 500–800 bp and 800–1000 bp were purified and ligated to pZero® -1 cloning vector. .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Article Title: Epigenetic Silencing of CRABP2 and MX1 in Head and Neck Tumors 1 in Head and Neck Tumors 1 2
Article Snippet: The fragments were inserted into Xho I-digested pZERO plasmid (1 µg/µl) at 16°C for 3 hours. .. Inserts were sequenced with forward and reverse M13 primers using a DYEnamic ET Dye Terminator Sequencing kit (Amersham Biosciences, Piscataway, NJ) and a MegaBACE 1000 sequencer (Amersham Biosciences).

Software:

Article Title: Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand
Article Snippet: Sequencing reactions were performed by using a DYEnamic ET dye terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions. .. Overlapping nucleic acid sequences were combined for analysis and edited by using SEQUENCHER software (Gene Code Corporation).

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions. .. Overlapping nucleic acid sequences were combined for analysis and edited using SEQUENCHER software (Gene Code Corporation).

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions. .. The overlapping sequences obtained from forward and reverse primers were combined for analysis and edited by using the Sequencher software (Gene Code Corporation).

Agarose Gel Electrophoresis:

Article Title: Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history
Article Snippet: For these three segments, the PCR conditions were identical and began with one step of 94°C for 3 min, 5 cycles (Touchdown) of 94°C for 45 s, 55-51°C for 45 s, 72°C for 1 min 30 s followed by 40 cycles of 94°C for 45 s, 50°C for 30 s, 72°C for 1 min 30 s and final extension of 72°C for 30 min. Thermocycling conditions for the ATP8 gene consisted of an initial denaturing step at 94°C for 3 min followed by 35 cycles of 94°C for 45 s, 50°C for 45 s, 72°C for 1 min 30 s and final extension of 72°C for 10 min. PCR products were visualized on a 1% agarose gel stained with GelRed® (Biotium Inc.) and then purified using a protocol based on precipitation with ammonium acetate and isopropanol, or the enzymatic method with the enzymes Exonuclease I (EXO I) and Shrimp Alkaline Phosphatase (SAP). .. Both strands of each PCR product were sequenced using the DYEnamic ET Dye Terminator Sequencing Kit (GE Healthcare) and analyzed in a MegaBACE 1000 automated sequencer (GE Healthcare).

Ethanol Precipitation:

Article Title: Overexpression of ANXA1 in Penile Carcinomas Positive for High-Risk HPVs
Article Snippet: Aliquots (10 µg) of the tester PCR products (penile carcinoma or normal tissue) were digested with 20 units of XhoI (Invitrogen Life Technologies, California, USA) and purified with phenol-chloroform extraction and ethanol precipitation. .. The sequences of these clones were determined using a DNA sequencer (ABI PRISM 377, Applied Biosystems, California, USA) and DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, New Jersey, USA).

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions. .. The sequencing products were purified by standard ethanol precipitation before sequencing in a MegaBACE 500 automated DNA sequencer (GE Healthcare).

Colony Assay:

Article Title: Searching for molecular markers in head and neck squamous cell carcinomas (HNSCC) by statistical and bioinformatic analysis of larynx-derived SAGE libraries
Article Snippet: Cells were plated on low salt LB agar containing Zeocin® , in plates compatible with the automated colony picker QPix2 (Genetix, New Milton, Hampshire, UK). .. Amplified inserts were checked and sequenced with forward M13 primer in a MegaBACE™1000 sequencer (Amersham Biosciences, Piscataway, NJ, USA) and the DYEnamic ET Dye Terminator Sequencing Kit (Amersham Biosciences, Piscataway, NJ, USA), or alternatively, an ABI PRISM® 377 DNA Sequencer (Applied Biosystems, Foster City, CA) and the ABI PRISM® BigDye™ Primer Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

Virus Isolation Assay:

Article Title: Frequent In-Migration and Highly Focal Transmission of Dengue Viruses among Children in Kamphaeng Phet, Thailand
Article Snippet: Paragraph title: Virus isolation and sequencing ... Sequencing reactions were performed using the DYEnamic ET Dye Terminator sequencing kit (GE Healthcare Bio-Sciences) according to the manufacturer's instructions.

Gel Extraction:

Article Title: Dengue-1 Virus Clade Replacement in Thailand Associated with Enhanced Mosquito Transmission
Article Snippet: The DNA fragments of the E gene and the overlapping DNA fragments covering the entire DENV-1 genome were amplified using AmpliTaq DNA polymerase (Applied Biosystems) and purified using the QIAquick PCR purification kit and QIAquick gel extraction kit (Qiagen). .. Purified DNA fragments were used as templates in cycle sequencing reactions using the DYEnamic ET dye terminator sequencing kit (GE Healthcare) according to the manufacturer's instructions.

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    GE Healthcare dyenamic et dye terminator sequencing kit
    Dyenamic Et Dye Terminator Sequencing Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 14 article reviews
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