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Millipore dye thioflavin s
Injury Patterns on Histology. Images show TTC, <t>Thioflavin</t> S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )
Dye Thioflavin S, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Hemorrhage promotes inflammation and myocardial damage following acute myocardial infarction: insights from a novel preclinical model and cardiovascular magnetic resonance"

Article Title: Hemorrhage promotes inflammation and myocardial damage following acute myocardial infarction: insights from a novel preclinical model and cardiovascular magnetic resonance

Journal: Journal of Cardiovascular Magnetic Resonance

doi: 10.1186/s12968-017-0361-7

Injury Patterns on Histology. Images show TTC, Thioflavin S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )
Figure Legend Snippet: Injury Patterns on Histology. Images show TTC, Thioflavin S and H E stained short axis sections obtained from explanted hearts at 24 h post intervention. TTC sections indicate region on necrosis appearing white in the I-HEM group (no hemorrhage) and reddish white in I+HEM group (hemorrhage); the +HEM group did no show infarction. Under ultra-violet light, non-fluorescent regions on the Thioflavin S stain highlight areas of compromised endothelium as seen in the +HEM and I+HEM groups; this confirms presence of microvascular damage in the hemorrhage groups. The H E images were obtained from the region of interest shown on the TTC stained sections. Hemorrhage was apparent in the +HEM and I+HEM groups as evidenced from the interstitial distribution of red blood cells ( open arrow heads ) in the affected LAD territory; red blood cells were absent in the I-HEM group. Edematous development was observed in all three groups ( arrows ) along with the presence of inflammatory cells ( closed arrow heads )

Techniques Used: Staining

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    Millipore thioflavin s
    Rac1 activity decreases α-SYN accumulation and aggregation in the neuroblastoma cell line BE(2)-M17. a Representative confocal images of α-SYN over-expressing cells induced with 10 μM retinoic acid (RA) and treated with 10 mM sodium butyrate (SB) for 36 h. Cells were transduced with Control-GFP (upper row), RAC1 (WT)-GFP (middle row) and RAC1 (CA)-GFP (bottom row), and co-stained for <t>Thioflavin</t> S (green) and α-SYN (red). Arrows indicate Thioflavin S positive aggregates with amyloidal structure. b Bar graph showing the quantitative analyses of the neuronal soma area (in percentage %) covered by Thioflavin S positive stain in individual cells transduced with (WT)- or (CA) RAC1 or with the corresponding control. N = 14 (EV), N = 25 (WT), and N = 24 (CA), from at least three independent experiments. Data are presented as mean ± SEM. Statistics, *** P
    Thioflavin S, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thioflavin s/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thioflavin s - by Bioz Stars, 2022-10
    97/100 stars
      Buy from Supplier

    97
    Millipore thioflavin s solution
    IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).
    Thioflavin S Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thioflavin s solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    thioflavin s solution - by Bioz Stars, 2022-10
    97/100 stars
      Buy from Supplier

    Image Search Results


    Rac1 activity decreases α-SYN accumulation and aggregation in the neuroblastoma cell line BE(2)-M17. a Representative confocal images of α-SYN over-expressing cells induced with 10 μM retinoic acid (RA) and treated with 10 mM sodium butyrate (SB) for 36 h. Cells were transduced with Control-GFP (upper row), RAC1 (WT)-GFP (middle row) and RAC1 (CA)-GFP (bottom row), and co-stained for Thioflavin S (green) and α-SYN (red). Arrows indicate Thioflavin S positive aggregates with amyloidal structure. b Bar graph showing the quantitative analyses of the neuronal soma area (in percentage %) covered by Thioflavin S positive stain in individual cells transduced with (WT)- or (CA) RAC1 or with the corresponding control. N = 14 (EV), N = 25 (WT), and N = 24 (CA), from at least three independent experiments. Data are presented as mean ± SEM. Statistics, *** P

    Journal: Molecular Neurobiology

    Article Title: The Small GTPase RAC1/CED-10 Is Essential in Maintaining Dopaminergic Neuron Function and Survival Against α-Synuclein-Induced Toxicity

    doi: 10.1007/s12035-018-0881-7

    Figure Lengend Snippet: Rac1 activity decreases α-SYN accumulation and aggregation in the neuroblastoma cell line BE(2)-M17. a Representative confocal images of α-SYN over-expressing cells induced with 10 μM retinoic acid (RA) and treated with 10 mM sodium butyrate (SB) for 36 h. Cells were transduced with Control-GFP (upper row), RAC1 (WT)-GFP (middle row) and RAC1 (CA)-GFP (bottom row), and co-stained for Thioflavin S (green) and α-SYN (red). Arrows indicate Thioflavin S positive aggregates with amyloidal structure. b Bar graph showing the quantitative analyses of the neuronal soma area (in percentage %) covered by Thioflavin S positive stain in individual cells transduced with (WT)- or (CA) RAC1 or with the corresponding control. N = 14 (EV), N = 25 (WT), and N = 24 (CA), from at least three independent experiments. Data are presented as mean ± SEM. Statistics, *** P

    Article Snippet: After incubation with the antibodies, coverslips were immersed in 0.005% thioflavin S (Sigma-Aldrich, Madrid, Spain) in PBS for 8 min and then rinsed twice in ethanol 70% and once in PBS.

    Techniques: Activity Assay, Expressing, Transduction, Staining

    Insoluble proteins form NFT-like aggregates rapidly after OA injection at both the injection site and distal brain regions. a β-pleated sheet protein structures are identified by thioflavin-S. Intracellular aggregates are observed at both the injection site and distal sites in the somatosensory cortex at 24 h, which persist 7 days post injection. Scale bar = 50 μM. b High magnification of thioflavin-S positive cells in the amygdala and somatosensory cortex. Scale bar = 10 μm. c Coronal sections taken from OA injected MAPT KO show no phospho-tau immunoreactivity. d No thioflavin positive structures are observed in MAPT KO mice with background staining comparable to the wild-type controls at 24 h. Scale bar = 50 μm

    Journal: Acta Neuropathologica Communications

    Article Title: A local insult of okadaic acid in wild-type mice induces tau phosphorylation and protein aggregation in anatomically distinct brain regions

    doi: 10.1186/s40478-016-0300-0

    Figure Lengend Snippet: Insoluble proteins form NFT-like aggregates rapidly after OA injection at both the injection site and distal brain regions. a β-pleated sheet protein structures are identified by thioflavin-S. Intracellular aggregates are observed at both the injection site and distal sites in the somatosensory cortex at 24 h, which persist 7 days post injection. Scale bar = 50 μM. b High magnification of thioflavin-S positive cells in the amygdala and somatosensory cortex. Scale bar = 10 μm. c Coronal sections taken from OA injected MAPT KO show no phospho-tau immunoreactivity. d No thioflavin positive structures are observed in MAPT KO mice with background staining comparable to the wild-type controls at 24 h. Scale bar = 50 μm

    Article Snippet: Slides were incubated in filtered 1 % thioflavin-S (Sigma) in 80 % ethanol for 15 min at room temperature (RT), protected from light.

    Techniques: Injection, Mouse Assay, Staining

    EFhd2 forms amyloid structures in vitro A , Various concentrations of purified, recombinant His EFhd2 WT (7.5, 30, 40μM) were incubated at 37°C with heparin and DTT (dash, dotted, solid line, respectively). After 20 hrs, samples were added Thioflavin-S (ThioS) and intensity was read in a fluorimeter at Exc.440 nm and Em.550 nm. ThioS stain has a high affinity for cross-β containing structures such as amyloids. EFhd2 showed affinity for this stain and an increase in ThioS binding was observed in a concentration-dependent manner. Baseline ThioS signal of buffer with heparin and DTT is shown (gray line) for comparison with EFhd2-containing reactions. B, Recombinant His EFhd2 WT (40μM) can also bind ThioS stain when incubated without heparin. Formation of cross-β structures by His EFhd2 WT was affected upon the addition of calcium, as it shows a reduction in the binding of ThioS (solid line, minus calcium; dashed lines, plus 1mM CaCl 2 ). Baseline ThioS signal of buffers with heparin and DTT minus (gray line) and plus (dotted line) 1mM CaCl 2 are shown for comparison with protein-containing reactions.

    Journal: Journal of neurochemistry

    Article Title: EFHD2 IS A NOVEL AMYLOID PROTEIN ASSOCIATED TO PATHOLOGICAL TAU IN ALZHEIMER'S DISEASE

    doi: 10.1111/jnc.12155

    Figure Lengend Snippet: EFhd2 forms amyloid structures in vitro A , Various concentrations of purified, recombinant His EFhd2 WT (7.5, 30, 40μM) were incubated at 37°C with heparin and DTT (dash, dotted, solid line, respectively). After 20 hrs, samples were added Thioflavin-S (ThioS) and intensity was read in a fluorimeter at Exc.440 nm and Em.550 nm. ThioS stain has a high affinity for cross-β containing structures such as amyloids. EFhd2 showed affinity for this stain and an increase in ThioS binding was observed in a concentration-dependent manner. Baseline ThioS signal of buffer with heparin and DTT is shown (gray line) for comparison with EFhd2-containing reactions. B, Recombinant His EFhd2 WT (40μM) can also bind ThioS stain when incubated without heparin. Formation of cross-β structures by His EFhd2 WT was affected upon the addition of calcium, as it shows a reduction in the binding of ThioS (solid line, minus calcium; dashed lines, plus 1mM CaCl 2 ). Baseline ThioS signal of buffers with heparin and DTT minus (gray line) and plus (dotted line) 1mM CaCl 2 are shown for comparison with protein-containing reactions.

    Article Snippet: After incubation, 0.5mM Thioflavin-S (SIGMA) was added to the samples and analyzed in a fluorimeter (Cary Eclipse fluorimeter, Agilent Technologies) with a temperature-controlled cell holder set at 37°C.

    Techniques: In Vitro, Purification, Recombinant, Incubation, Staining, Binding Assay, Concentration Assay

    IL-1Ra immunoreactivity is detectable in human islet α-cells. (A)  Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green).  (B)  Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: IL-1Ra immunoreactivity is detectable in human islet α-cells. (A) Paraffin-embedded sections from human islets transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) and cultured (11.1 mmol/L glucose) for 7 days were immunolabeled for glucagon (red) and IL-1Ra (green). (B) Triple immunostaining of pre-culture human islets for insulin (blue), glucagon (red) and IL-1Ra (green). The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of the corresponding images. Amyloid formation during islet culture is shown as an insert in merged micrographs (top right, insulin; red and thioflavin S; blue). Scale bar = 50 μm; inserts: ×3. Micrographs are representative of four independent studies (4 human islet preparations).

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Transduction, Cell Culture, Immunolabeling, Triple Immunostaining

    IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: IL-1Ra levels are increased in oligomer-positive islet areas and reduced in thioflavin S-positive islet areas. (A) Paraffin embedded sections from 7-day cultured (11.1 mmol/L glucose) non-transduced or transduced with Ad-prohIAPP-siRNA (or Ad-control-siRNA) islets were immunolabeled for insulin (red) and IL-1Ra (green). The squares (dashed white lines) denote enlarged regions shown as inserts. (B) Human islets were immunolabeled for IL-1Ra (green) and A11 (red; top panel) or insulin (red), IL-1Ra (green) and thioflavin S (Thio S; blue; bottom panel). (C) Paraffin-embedded sections from (left to right): ob/ob mouse and human adipose tissue immunolabeled for IL-1Ra (green; positive control); human islets incubated with secondary antibody alone (negative control) and anakinra-treated human islets immunolabeled for insulin (red) and IL-1Ra (green). Scale bar = 50 μm; inserts: ×3 (A11: ×4). Micrographs are representative of four independent studies (4 human islet preparations).

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Cell Culture, Transduction, Immunolabeling, Positive Control, Incubation, Negative Control

    Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p

    Journal: Molecular Metabolism

    Article Title: Amyloid formation disrupts the balance between interleukin-1β and interleukin-1 receptor antagonist in human islets

    doi: 10.1016/j.molmet.2017.05.016

    Figure Lengend Snippet: Treatment with neutralizing IL-1β antibody markedly reduces islet IL-1β immunoreactivity and prevents β-cell Fas upregulation induced by amyloid formation in cultured human islets. Human islets non-transduced or transduced with Ad-prohIAPP-siRNA or Ad-control-siRNA (MOI: 20) were cultured with (+nIL1β) or without (-nIL1β) a neutralizing monoclonal human IL-1β antibody (1 μg/ml) in CMRL (11.1 mmol/L glucose) for 7 days. (A) Paraffin-embedded islet sections were immunolabeled for insulin (red), IL-1β or Fas (green) and thioflavin S (Thio S; blue) as indicated. The squares (dashed white lines) denote regions enlarged and depicted as inserts at the bottom right of each image. The top right inserts in each micrograph show immunolabeling for insulin (green) and A11 (red). Scale bar = 50 μm; inserts: ×3 (A11: ×4). (B) The proportion of Fas positive islet β-cells (fold over day 0). The percentage of (C) A11 (oligomer)-positive islets, (D) thioflavin S (amyloid) positive islets, and (E) amyloid area to total islet area. Data are presented as mean ± SEM of four independent studies (4 human islet preparations); n = 15–20 islets per condition in each study. *vs day 0; #vs corresponding non-treated group ( p

    Article Snippet: For thioflavin S staining, sections were incubated with 0.5% (wt/vol.) thioflavin S solution (Sigma–Aldrich, Oakville, ON, Canada) for 5 min at room temperature.

    Techniques: Cell Culture, Transduction, Immunolabeling