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Thermo Fisher dye dtt
Dye Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dye dtt/product/Thermo Fisher
Average 84 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dye dtt - by Bioz Stars, 2020-03
84/100 stars

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tca precipitation

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TCA Precipitation:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: .. For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M). .. For native PAGE, supernatants were treated by addition of Proteinase Inhibitor Cocktail VII (Calbiochem) at a dilution of 50:1, and concentrated 100-fold using Corning® Spin-X® UF concentrators (6 ml, 10,000 MWCO) at 4000g in a swing bucket rotor.

Cell Culture:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: Expression of cellulosomes for western blot analysis C. acetobutylicum strains were cultured for protein expression analysis in 2xYTG medium buffered to pH 7 with 40 mM MOPS as previously described [ ]. .. For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M).

Concentration Assay:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: .. For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M). .. For native PAGE, supernatants were treated by addition of Proteinase Inhibitor Cocktail VII (Calbiochem) at a dilution of 50:1, and concentrated 100-fold using Corning® Spin-X® UF concentrators (6 ml, 10,000 MWCO) at 4000g in a swing bucket rotor.

Incubation:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: Overnight cultures in the exponential phase of growth were used to inoculate 30 ml 2xYTG + 40 mM MOPS pH 7 to an OD600 of 0.05; these cultures were incubated in the anaerobic cabinet until the OD600 had reached approximately 0.8. .. For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M).

Expressing:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: Paragraph title: Expression of cellulosomes for western blot analysis ... For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M).

Western Blot:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: Paragraph title: Expression of cellulosomes for western blot analysis ... For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M).

Clear Native PAGE:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M). .. For native PAGE, supernatants were treated by addition of Proteinase Inhibitor Cocktail VII (Calbiochem) at a dilution of 50:1, and concentrated 100-fold using Corning® Spin-X® UF concentrators (6 ml, 10,000 MWCO) at 4000g in a swing bucket rotor.

SDS Page:

Article Title: Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824
Article Snippet: .. For SDS-PAGE, 20 ml supernatant samples were concentrated 100-fold via TCA precipitation as described by Schwarz et al. [ ] and resuspended in 2 × loading dye + DTT (1:1 dilution of Thermo-Fisher NuPAGE® LDS Sample Buffer (4X) with 1 M Tris pH 8, with DTT added to a concentration of 0.2 M). .. For native PAGE, supernatants were treated by addition of Proteinase Inhibitor Cocktail VII (Calbiochem) at a dilution of 50:1, and concentrated 100-fold using Corning® Spin-X® UF concentrators (6 ml, 10,000 MWCO) at 4000g in a swing bucket rotor.

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    Thermo Fisher dye dtt
    Dye Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dye dtt/product/Thermo Fisher
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dye dtt - by Bioz Stars, 2020-03
    84/100 stars
      Buy from Supplier

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