Structured Review

Cellgro dulbecco s pbst
Dulbecco S Pbst, supplied by Cellgro, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dulbecco s pbst/product/Cellgro
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
dulbecco s pbst - by Bioz Stars, 2020-11
85/100 stars

Related Products / Commonly Used Together

heat inactivated goat serum

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Related Articles

Incubation:

Article Title: Isolation and Proteomic Characterization of the Mouse Sperm Acrosomal Matrix *
Article Snippet: .. After washing in TBS (50 m m Tris-HCl pH 7.4, 150 m m NaCl) for 10 min at RT, slides were incubated in 100% heat inactivated goat serum (HIGS, cat.no.S-1000, Vector Laboratories, Burlingame, CA) for 1 h at 37 °C, then washed in Dulbecco's PBST (DPBS containing 1 m m CaCl2 , 0.5 m m MgCl2 (cat.no.21-030, Cellgro, Manassas, VA) and 0.1% Tween-20 (cat.no.P1379, Sigma, Saint Louis, MO)) for 7 min at RT. .. Slides were incubated with primary antibody (3 μg/ml affinity purified rabbit anti-mouse zonadhesin (ZAN), 1:1000 rabbit anti-guinea pig acrosin (ACR) and 1:1000 rabbit anti-mouse acrosin binding protein (ACRBP)) diluted in DPBS containing 5% HIGS for 1 h at 37 °C.

Chloramphenicol Acetyltransferase Assay:

Article Title: Isolation and Proteomic Characterization of the Mouse Sperm Acrosomal Matrix *
Article Snippet: .. After washing in TBS (50 m m Tris-HCl pH 7.4, 150 m m NaCl) for 10 min at RT, slides were incubated in 100% heat inactivated goat serum (HIGS, cat.no.S-1000, Vector Laboratories, Burlingame, CA) for 1 h at 37 °C, then washed in Dulbecco's PBST (DPBS containing 1 m m CaCl2 , 0.5 m m MgCl2 (cat.no.21-030, Cellgro, Manassas, VA) and 0.1% Tween-20 (cat.no.P1379, Sigma, Saint Louis, MO)) for 7 min at RT. .. Slides were incubated with primary antibody (3 μg/ml affinity purified rabbit anti-mouse zonadhesin (ZAN), 1:1000 rabbit anti-guinea pig acrosin (ACR) and 1:1000 rabbit anti-mouse acrosin binding protein (ACRBP)) diluted in DPBS containing 5% HIGS for 1 h at 37 °C.

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    Cellgro dulbecco s pbst
    Dulbecco S Pbst, supplied by Cellgro, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dulbecco s pbst/product/Cellgro
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dulbecco s pbst - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

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