dubos oleic albumin complex enriched middlebrook 7h9 liquid medium  (MiddleBrook Pharmaceuticals)

 
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    MiddleBrook Pharmaceuticals dubos oleic albumin complex enriched middlebrook 7h9 liquid medium
    Dubos Oleic Albumin Complex Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dubos oleic albumin complex enriched middlebrook 7h9 liquid medium/product/MiddleBrook Pharmaceuticals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dubos oleic albumin complex enriched middlebrook 7h9 liquid medium - by Bioz Stars, 2020-04
    86/100 stars

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    Centrifugation:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: .. To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. Viable bacterial counts were determined by plating serial 10-fold dilutions on Middlebrook 7H10 (Difco) agar plates.

    Isolation:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: .. To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. Viable bacterial counts were determined by plating serial 10-fold dilutions on Middlebrook 7H10 (Difco) agar plates.

    Size-exclusion Chromatography:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. For infections, the stock solution was thawed, briefly sonicated (10 sec) to disaggregate clumps, and diluted to a desired concentration (50- to 200-fold) in PBS containing 0.05% Tween 80.

    Infection:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: .. To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. Viable bacterial counts were determined by plating serial 10-fold dilutions on Middlebrook 7H10 (Difco) agar plates.

    Mouse Assay:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: .. To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. Viable bacterial counts were determined by plating serial 10-fold dilutions on Middlebrook 7H10 (Difco) agar plates.

    Concentration Assay:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. For infections, the stock solution was thawed, briefly sonicated (10 sec) to disaggregate clumps, and diluted to a desired concentration (50- to 200-fold) in PBS containing 0.05% Tween 80.

    Sonication:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. For infections, the stock solution was thawed, briefly sonicated (10 sec) to disaggregate clumps, and diluted to a desired concentration (50- to 200-fold) in PBS containing 0.05% Tween 80.

    Injection:

    Article Title: Genetic control of resistance to experimental infection with virulent Mycobacterium tuberculosis
    Article Snippet: To prepare frozen stocks for experiments, mycobacteria were isolated from the organs of infected mice, grown in Dubos oleic albumin complex-enriched Middlebrook 7H9 liquid medium (Difco) containing 0.05% Tween 80 in roller bottles, pelleted by centrifugation (800 × g for 10 min), and resuspended in PBS containing 0.05% Tween 80 (PBS-Tween 80). .. Bacterial suspensions were injected through the lateral tail vein in a final volume of 100 μl.

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    MiddleBrook Pharmaceuticals dubos oleic albumin complex enriched middlebrook 7h9 liquid medium
    Dubos Oleic Albumin Complex Enriched Middlebrook 7h9 Liquid Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dubos oleic albumin complex enriched middlebrook 7h9 liquid medium/product/MiddleBrook Pharmaceuticals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dubos oleic albumin complex enriched middlebrook 7h9 liquid medium - by Bioz Stars, 2020-04
    86/100 stars
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