prostate carcinoma cell line du145  (ATCC)


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    ATCC prostate carcinoma cell line du145
    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing <t>DU145</t> cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Prostate Carcinoma Cell Line Du145, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The cation channel TRPM8 influences the differentiation and function of human monocytes"

    Article Title: The cation channel TRPM8 influences the differentiation and function of human monocytes

    Journal: Journal of Leukocyte Biology

    doi: 10.1002/JLB.1HI0421-181R

    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Figure Legend Snippet: TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa

    Techniques Used: Cell Culture, Expressing, Positive Control, Flow Cytometry, Staining, Blocking Assay, Fluorescence, Real-time Polymerase Chain Reaction, RNA Expression, MANN-WHITNEY, Immunohistochemistry

    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing <t>DU145</t> CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CRISPR/Cas9 screenings unearth protein arginine methyltransferase 7 as a novel driver of metastasis in prostate cancer"

    Article Title: CRISPR/Cas9 screenings unearth protein arginine methyltransferase 7 as a novel driver of metastasis in prostate cancer

    Journal: bioRxiv

    doi: 10.1101/2023.07.20.549704

    A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing DU145 CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.
    Figure Legend Snippet: A, Graphical scheme of the experimental design of CRISPR/Cas9 screenings . B, Volcano plot showing PC3 CRISPR/Cas9 screening results. C, Volcano plot showing DU145 CRISPR/Cas9 screening results. D, Venn diagram showing the number of genes significantly associated with PCa invasive process in each line and the number of common genes between both screenings.

    Techniques Used: CRISPR

    Gene ontology biological pathway enrichment analysis (GO:BP) using Metascape of (A) PC3 and (B) DU145 results. C-D, Biological pathways GSEA in (C) PC3 and (D) DU145 screening results.
    Figure Legend Snippet: Gene ontology biological pathway enrichment analysis (GO:BP) using Metascape of (A) PC3 and (B) DU145 results. C-D, Biological pathways GSEA in (C) PC3 and (D) DU145 screening results.

    Techniques Used:

    A, Invasion assay of PC3 inhibited cells using specific siRNA to target our best gene candidates versus control (siCTL) cells. B-C, Representative western blot of PRMT7, PRMT5 and β-actin protein levels in (B) PC3-Cas9 and (C) DU145-Cas9 cell lines. The numbers below each lane represent PRMT7/β-actin or PRMT5/β-actin respectively densitometric quantification is referred to control cells. (n=3). D-E, Invasion assay of PRMT7 depleted versus control (CTL) cells of (D) PC3-Cas9 and (E) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001). F-G, Migration assay of PRMT7 depleted versus CTL cells of (F) PC3-Cas9 and (G) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). H-I, Viability assay of PRMT7 depleted versus CTL cells of (H) PC3- Cas9 and (I) DU145-Cas9 cells (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: A, Invasion assay of PC3 inhibited cells using specific siRNA to target our best gene candidates versus control (siCTL) cells. B-C, Representative western blot of PRMT7, PRMT5 and β-actin protein levels in (B) PC3-Cas9 and (C) DU145-Cas9 cell lines. The numbers below each lane represent PRMT7/β-actin or PRMT5/β-actin respectively densitometric quantification is referred to control cells. (n=3). D-E, Invasion assay of PRMT7 depleted versus control (CTL) cells of (D) PC3-Cas9 and (E) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001). F-G, Migration assay of PRMT7 depleted versus CTL cells of (F) PC3-Cas9 and (G) DU145-Cas9 cells (mean ± SEM of n=3 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). H-I, Viability assay of PRMT7 depleted versus CTL cells of (H) PC3- Cas9 and (I) DU145-Cas9 cells (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Invasion Assay, Western Blot, Migration, Viability Assay

    A, Volcano plot showing differentially expressed genes (DEGs) (adjusted p-value < 0.05, |logFC| > 1) from the differential gene expression analysis of PC3 control versus PRMT7 depleted cells results, and a barplot representing the number of genes that significantly changed their expression, accounting for the number of genes that were upregulated (red) and downregulated (blue) in PRMT7 depleted cells. B, Over-representation analysis of biological process GO terms in DEGs (adjusted p-value < 0.05, |logFC| > 1) of PRMT7 depleted cells compared to control cells, representing top 25 terms with highest gene ratio with an adjusted p-value cutoff of 0.05 and redundant GO terms were removed. C, Heatmap showing the gene expression (Z-score) of the genes found in parental GO term cell adhesion sorted by log 2 fold change. Horizontal lines denote DEG positions, and those of interest are highlighted in red. D-E, Western blot analysis of ITGα1 and ITGβ4 protein levels in (D) PC3-Cas9 and (E) DU145-Cas9 cells. The numbers below each lane represent ITGα1/β-actin or ITGβ4/β-actin densitometric quantification referred to control cells, respectively. F-G, Graph representing mean number of cells per field adhered to (F) collagen IV or (G) laminin (mean ± SEM of n=3, by unpaired Student’s t test *P<0.05, **P>0.01).
    Figure Legend Snippet: A, Volcano plot showing differentially expressed genes (DEGs) (adjusted p-value < 0.05, |logFC| > 1) from the differential gene expression analysis of PC3 control versus PRMT7 depleted cells results, and a barplot representing the number of genes that significantly changed their expression, accounting for the number of genes that were upregulated (red) and downregulated (blue) in PRMT7 depleted cells. B, Over-representation analysis of biological process GO terms in DEGs (adjusted p-value < 0.05, |logFC| > 1) of PRMT7 depleted cells compared to control cells, representing top 25 terms with highest gene ratio with an adjusted p-value cutoff of 0.05 and redundant GO terms were removed. C, Heatmap showing the gene expression (Z-score) of the genes found in parental GO term cell adhesion sorted by log 2 fold change. Horizontal lines denote DEG positions, and those of interest are highlighted in red. D-E, Western blot analysis of ITGα1 and ITGβ4 protein levels in (D) PC3-Cas9 and (E) DU145-Cas9 cells. The numbers below each lane represent ITGα1/β-actin or ITGβ4/β-actin densitometric quantification referred to control cells, respectively. F-G, Graph representing mean number of cells per field adhered to (F) collagen IV or (G) laminin (mean ± SEM of n=3, by unpaired Student’s t test *P<0.05, **P>0.01).

    Techniques Used: Expressing, Western Blot

    A Hematoxylin and eosin staining of PCa primary tumor slides showing the aggressiveness of tumors measured by Gleason score PRMT7 (scale bars: 50 μM). B, PRMT7 gene expression levels in PCa primary tumor samples with higher (n=8) versus lower (n=5) Gleason score samples (mean ± SEM of n=13 primary tumor samples, by unpaired Mann-Whitney test *p < 0.05, **p < 0.01, ***p < 0.001). C, D, Representative western blot of arginine monomethylation (MMA) levels in (C) PC3 and (D) DU145 cells treated with 10μM of SGC3027 (PRMT7 inhibitor). The numbers below each lane represent MMA/β-actin densitometric quantification referred to control cells. E-F, Invasion assay of PRMT7 inhibited versus vehicle cells of (E) PC3 and (F) DU145 cells (mean ± SEM of n=6 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). G-H, Cell viability assay of PRMT7 inhibited versus vehicle cells of (G) PC3 and (H) DU145 cell lines (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
    Figure Legend Snippet: A Hematoxylin and eosin staining of PCa primary tumor slides showing the aggressiveness of tumors measured by Gleason score PRMT7 (scale bars: 50 μM). B, PRMT7 gene expression levels in PCa primary tumor samples with higher (n=8) versus lower (n=5) Gleason score samples (mean ± SEM of n=13 primary tumor samples, by unpaired Mann-Whitney test *p < 0.05, **p < 0.01, ***p < 0.001). C, D, Representative western blot of arginine monomethylation (MMA) levels in (C) PC3 and (D) DU145 cells treated with 10μM of SGC3027 (PRMT7 inhibitor). The numbers below each lane represent MMA/β-actin densitometric quantification referred to control cells. E-F, Invasion assay of PRMT7 inhibited versus vehicle cells of (E) PC3 and (F) DU145 cells (mean ± SEM of n=6 biological replicates, by unpaired Student’s t test *p < 0.05, **p < 0.01, ***p < 0.001). G-H, Cell viability assay of PRMT7 inhibited versus vehicle cells of (G) PC3 and (H) DU145 cell lines (mean ± SEM of n=9 biological replicates, by TWO-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

    Techniques Used: Staining, Expressing, MANN-WHITNEY, Western Blot, Invasion Assay, Viability Assay

    prostate cancer pca cell lines du145 atcc htb 81  (ATCC)


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    ATCC prostate cancer pca cell lines du145 atcc htb 81
    Advanced <t>prostate</t> <t>cancer</t> <t>cells</t> secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and <t>DU145</t> supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Prostate Cancer Pca Cell Lines Du145 Atcc Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells"

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    Journal: Journal of Immunology Research

    doi: 10.1155/2022/1810804

    Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Figure Legend Snippet: Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Techniques Used: Concentration Assay, Cell Culture

    The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Figure Legend Snippet: The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Techniques Used: Expressing, Cell Culture, Flow Cytometry

    Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.
    Figure Legend Snippet: Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Techniques Used: De-Phosphorylation Assay, Cell Culture, Expressing, Western Blot, In-Cell ELISA, Activity Assay, Concentration Assay

    Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.
    Figure Legend Snippet: Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Techniques Used:

    cell lines du145 htb 81  (ATCC)


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    ATCC cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines du145 htb 81 - by Bioz Stars, 2024-09
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    1) Product Images from "Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines"

    Article Title: Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064426

    DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Figure Legend Snippet: DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Techniques Used: Staining, Incubation, Flow Cytometry

    human pca cell lines du145 htb 81  (ATCC)


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    ATCC human pca cell lines du145 htb 81
    Human Pca Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prostate carcinoma cell line du145  (ATCC)


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    ATCC prostate carcinoma cell line du145
    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing <t>DU145</t> cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Prostate Carcinoma Cell Line Du145, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "The cation channel TRPM8 influences the differentiation and function of human monocytes"

    Article Title: The cation channel TRPM8 influences the differentiation and function of human monocytes

    Journal: Journal of Leukocyte Biology

    doi: 10.1002/JLB.1HI0421-181R

    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Figure Legend Snippet: TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa

    Techniques Used: Cell Culture, Expressing, Positive Control, Flow Cytometry, Staining, Blocking Assay, Fluorescence, Real-time Polymerase Chain Reaction, RNA Expression, MANN-WHITNEY, Immunohistochemistry

    du145 htb 81 cell lines  (ATCC)


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    ATCC du145 htb 81 cell lines
    Expression of TPD52 in prostate cancer. ( a ) Expression of TPD52 in prostate cancer progression model. RWPE < WPE2-NA22 < WPE1-NB14 < WPE1-NB11 < WPE1-NB26 with increase in proliferation and progression of cancer protein expression of TPD52 also increased as depicted by Immunoblot analysis. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. ( b ) Expression of TPD52 was observed in seven prostate cancer cell lines (RWPE, LnCap, <t>Du145,</t> CWR22Rν1, PC3, C 4-2, and NB26) under regular culture conditions. Except for RWPE-1 all other cell lines tested showed marked protein expression of TPD52 in Immunoblot analysis. ( c ) Immunoblot analysis for expression of TPD52 in prostate cancer development and progression model (transgenic adenocarcinoma of the mouse prostate, TRAMP), showed an increase in expression with the development and progression of prostate cancer. The blots shown here are representative of three independent experiments with similar results.
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Tissue microarray profiling and integrative proteomics indicate the modulatory potential of Maytenus royleanus in inhibition of overexpressed TPD52 in prostate cancers"

    Article Title: Tissue microarray profiling and integrative proteomics indicate the modulatory potential of Maytenus royleanus in inhibition of overexpressed TPD52 in prostate cancers

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-91408-8

    Expression of TPD52 in prostate cancer. ( a ) Expression of TPD52 in prostate cancer progression model. RWPE < WPE2-NA22 < WPE1-NB14 < WPE1-NB11 < WPE1-NB26 with increase in proliferation and progression of cancer protein expression of TPD52 also increased as depicted by Immunoblot analysis. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. ( b ) Expression of TPD52 was observed in seven prostate cancer cell lines (RWPE, LnCap, Du145, CWR22Rν1, PC3, C 4-2, and NB26) under regular culture conditions. Except for RWPE-1 all other cell lines tested showed marked protein expression of TPD52 in Immunoblot analysis. ( c ) Immunoblot analysis for expression of TPD52 in prostate cancer development and progression model (transgenic adenocarcinoma of the mouse prostate, TRAMP), showed an increase in expression with the development and progression of prostate cancer. The blots shown here are representative of three independent experiments with similar results.
    Figure Legend Snippet: Expression of TPD52 in prostate cancer. ( a ) Expression of TPD52 in prostate cancer progression model. RWPE < WPE2-NA22 < WPE1-NB14 < WPE1-NB11 < WPE1-NB26 with increase in proliferation and progression of cancer protein expression of TPD52 also increased as depicted by Immunoblot analysis. The total cell lysate was prepared and 40 μg protein was subjected to SDS-page followed by Immunoblot analysis and chemiluminescence detection. ( b ) Expression of TPD52 was observed in seven prostate cancer cell lines (RWPE, LnCap, Du145, CWR22Rν1, PC3, C 4-2, and NB26) under regular culture conditions. Except for RWPE-1 all other cell lines tested showed marked protein expression of TPD52 in Immunoblot analysis. ( c ) Immunoblot analysis for expression of TPD52 in prostate cancer development and progression model (transgenic adenocarcinoma of the mouse prostate, TRAMP), showed an increase in expression with the development and progression of prostate cancer. The blots shown here are representative of three independent experiments with similar results.

    Techniques Used: Expressing, Western Blot, SDS Page, Transgenic Assay

    reference identifiers additional information cell line human du145 atcc htb 81 rrid cvcl 0105 cell line  (ATCC)


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    ATCC reference identifiers additional information cell line human du145 atcc htb 81 rrid cvcl 0105 cell line
    Reference Identifiers Additional Information Cell Line Human Du145 Atcc Htb 81 Rrid Cvcl 0105 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    du145 htb 81 human prostate cancer cell lines  (ATCC)


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    ATCC du145 htb 81 human prostate cancer cell lines
    Du145 Htb 81 Human Prostate Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    • prostate carcinoma cell line du145  (ATCC)
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    ATCC prostate carcinoma cell line du145
    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing <t>DU145</t> cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Prostate Carcinoma Cell Line Du145, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prostate carcinoma cell line du145/product/ATCC
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    du145 htb 81 cell lines  (ATCC)
    86
    ATCC du145 htb 81 cell lines
    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing <t>DU145</t> cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa
    Du145 Htb 81 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prostate cancer pca cell lines du145 atcc htb 81  (ATCC)
    86
    ATCC prostate cancer pca cell lines du145 atcc htb 81
    Advanced <t>prostate</t> <t>cancer</t> <t>cells</t> secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and <t>DU145</t> supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).
    Prostate Cancer Pca Cell Lines Du145 Atcc Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cell lines du145 htb 81  (ATCC)
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    ATCC cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human pca cell lines du145 htb 81  (ATCC)
    86
    ATCC human pca cell lines du145 htb 81
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Human Pca Cell Lines Du145 Htb 81, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    reference identifiers additional information cell line human du145 atcc htb 81 rrid cvcl 0105 cell line  (ATCC)
    86
    ATCC reference identifiers additional information cell line human du145 atcc htb 81 rrid cvcl 0105 cell line
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Reference Identifiers Additional Information Cell Line Human Du145 Atcc Htb 81 Rrid Cvcl 0105 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference identifiers additional information cell line human du145 atcc htb 81 rrid cvcl 0105 cell line/product/ATCC
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    du145 htb 81 human prostate cancer cell lines  (ATCC)
    86
    ATCC du145 htb 81 human prostate cancer cell lines
    <t>DU145</t> ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.
    Du145 Htb 81 Human Prostate Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    du145 htb 81 human prostate cancer cell lines - by Bioz Stars, 2024-09
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    TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa

    Journal: Journal of Leukocyte Biology

    Article Title: The cation channel TRPM8 influences the differentiation and function of human monocytes

    doi: 10.1002/JLB.1HI0421-181R

    Figure Lengend Snippet: TRPM8 is expressed in cells of the monocyte/macrophage lineage in humans. (A)–(C) Peripheral blood monocytes were cultured for 6 days in the presence of M‐CSF to obtain Mo‐M. The TRPM8‐expressing DU145 cell line was used as a positive control. (A) and (B) Flow cytometry analysis showing TRPM8 protein expression in permeabilized cells after staining with anti‐TRPM8 antibody alone or in combination with a specific blocking peptide (SBP). (A) Representative plots and (B) mean fluorescence intensity (MFI) of TRPM8 protein expression presented as a fold change compared with SBP control. (C) Quantitative real time PCR analysis showing TRPM8 RNA expression. Data are expressed as 2 –ΔCT by normalization to GAPDH. (B) and (C) Each dot represents data from an independent donor. Mean ± sd are indicated. * = p < 0.05, ns = p > 0.05 (Mann–Whitney). Dotted line = value from DU145 cell line (mean of 3 independent passages). (D) Representative immunohistochemistry image showing costaining (yellow) of TRPM8 (red) and CD64 (green) in inflamed human colonic mucosa

    Article Snippet: The prostate carcinoma cell line DU145 (ATCC) was propagated in complete growth medium (DMEM containing 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin) as per the recommended protocol.

    Techniques: Cell Culture, Expressing, Positive Control, Flow Cytometry, Staining, Blocking Assay, Fluorescence, Real-time Polymerase Chain Reaction, RNA Expression, MANN-WHITNEY, Immunohistochemistry

    Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Advanced prostate cancer cells secrete a higher concentration of growth factors and cytokines according to stage progression. The cells were cultured at different times, the supernatants from PCa lines were collected, and 13 cytokines and 10 growth factors were evaluated using LEGENDplex™ technology. (a, b) The concentration of growth factors and cytokines from the 22Rv1, LNCaP, and DU145 supernatant was evaluated at 12, 24, and 48 h. (c,e) The concentration of soluble molecules increases, conforming with the advances of stage progression; the highest VEGF, M-CSF, CXCL8, and IL-6 values were observed in the DU145 cell line. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: Concentration Assay, Cell Culture

    The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: The expression of CD155 is increased in DU145 cells. The cells were cultured, and the expression of the ligands was subsequently evaluated by flow cytometry. (a) Ten thousand events from the region of the live cells were implemented for ligand evaluation. (b, c) The average percentage expression and (d) MFI of the ligands in the three PCa cell lines are shown. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Tukey's multiple comparison test).

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: Expressing, Cell Culture, Flow Cytometry

    Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Stattic and tocilizumab combination increase pSTAT-3 dephosphorylation in DU145 cells. The cells were cultured with the different treatments according to the corresponding groups for 24 h. (a) IL6R/STAT-3 axis expression was compared between PCa cell lines using 50 μ g of protein by Western blot; the presence of constitutive pSTAT-3 expression was shown in the DU145 line only; protein expression was then verified by in-cell Western. (b) Use of treatment with Stt causes a decrease in metabolic activity with concentrations greater than 5 μ M. (c) The effective Stt decrease is only shown at concentrations above the IC50. (d) The combined treatments with Tcz allow a lower Stt concentration than the IC50 with greater efficiency in the dephosphorylation of pSTAT-3. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; (unpaired t -test) against the basal group. Stt: stattic; Tcz: tocilizumab.

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques: De-Phosphorylation Assay, Cell Culture, Expressing, Western Blot, In-Cell ELISA, Activity Assay, Concentration Assay

    Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Journal: Journal of Immunology Research

    Article Title: Combination Blockade of the IL6R/STAT-3 Axis with TIGIT and Its Impact on the Functional Activity of NK Cells against Prostate Cancer Cells

    doi: 10.1155/2022/1810804

    Figure Lengend Snippet: Stattic + Anti-TIGIT + Tocilizumab increase the cytotoxicity of NK-92 cells against DU145 prostate cancer cells. (a) Significant decrease in KT50 in the Stt + Anti-TIGIT + Tcz treated groups compared to the basal group. (b) A significant increase was observed in the percentage of cytolysis in the groups treated with Stt + Anti-TIGIT + Tcz compared with the basal group observed at 4 and 24 h of coculture in both ranges E : T. All experiments were repeated at least three times. Data are shown as the mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (ANOVA with Bonferroni multiple comparison test). Stt: stattic; Tcz: tocilizumab.

    Article Snippet: The prostate cancer (PCa) cell lines DU145/ATCC® HTB-81™ (metastatic tumor and castration-resistant), LNCaP clone FGC/ATCC® CRL1740™ (metastatic tumor and androgen-dependent), and 22Rv1/ATCC® CRL2505™ (non-metastatic tumor) were obtained from the ATCC® (Manassas, VA, USA).

    Techniques:

    DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Journal: PLoS ONE

    Article Title: Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

    doi: 10.1371/journal.pone.0064426

    Figure Lengend Snippet: DU145 ( A ) and PC3 ( B ) (1×10 5 cells) cell lines were seeded in 6-well plates, following the same protocol for apoptosis with annexin V/FITC and PI staining. Cells treated with CrataBL (40 µM), containing RPMI without FBS were incubated for 48 h at 37°C and 5% (v/v) CO 2 . The cells were incubated with 10 µL of cleaved caspase 3 Alexa Fluor 488-conjugated antibody for 40 min and analyzed in FACSCalibur flow cytometer. As control, the cells were treated with medium only. The area in black represents the control and in white, cells treated with CrataBL.

    Article Snippet: The cell lines DU145 (HTB-81™) and PC3 (CRL-1435™) were purchased from ATCC®.

    Techniques: Staining, Incubation, Flow Cytometry