du 145 atcc htb 81 rrid cvcl 0105  (ATCC)


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    ATCC du 145 atcc htb 81 rrid cvcl 0105
    Du 145 Atcc Htb 81 Rrid Cvcl 0105, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du 145 atcc htb 81 rrid cvcl 0105/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    du 145 atcc htb 81 rrid cvcl 0105 - by Bioz Stars, 2024-09
    86/100 stars

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    du 145 atcc htb 81 rrid cvcl 0105  (ATCC)


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    ATCC du 145 atcc htb 81 rrid cvcl 0105
    Gain and loss of function of BAX regulate CLU-mediated mitophagy during cisplatin treatment. (A, C) Western blot analysis in CLU-OE FaDu and <t>DU</t> <t>145</t> cells with loss of function (transfected with shNC, sh BAX ) or gain of function (transfected with vector, BAX ) of BAX followed by cisplatin treatment, respectively. (B i , D i ) Confocal microscopy-based live-cell imaging of cisplatin-treated CLU-OE FaDu and DU 145 cells with loss of function (transfected with shNC, sh BAX ) and gain of function (transfected with vector, BAX ) of BAX followed by labelling with LTR (red; representing lysosomes) and MTG (green; representing mitochondria). Scale bars: 25 µm. (B ii , D ii ) Quantification showing colocalization (%) defined as Pearson’s co-efficient value calculated from images of each condition of three independent experiments using the JACoP plugin of ImageJ. Data were normalized to the MOCK or shNC cells (mean ± S.D., n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.
    Du 145 Atcc Htb 81 Rrid Cvcl 0105, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du 145 atcc htb 81 rrid cvcl 0105/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    du 145 atcc htb 81 rrid cvcl 0105 - by Bioz Stars, 2024-09
    86/100 stars

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    1) Product Images from "CLU (clusterin) and PPARGC1A/PGC1α coordinately control mitophagy and mitochondrial biogenesis for oral cancer cell survival"

    Article Title: CLU (clusterin) and PPARGC1A/PGC1α coordinately control mitophagy and mitochondrial biogenesis for oral cancer cell survival

    Journal: Autophagy

    doi: 10.1080/15548627.2024.2309904

    Gain and loss of function of BAX regulate CLU-mediated mitophagy during cisplatin treatment. (A, C) Western blot analysis in CLU-OE FaDu and DU 145 cells with loss of function (transfected with shNC, sh BAX ) or gain of function (transfected with vector, BAX ) of BAX followed by cisplatin treatment, respectively. (B i , D i ) Confocal microscopy-based live-cell imaging of cisplatin-treated CLU-OE FaDu and DU 145 cells with loss of function (transfected with shNC, sh BAX ) and gain of function (transfected with vector, BAX ) of BAX followed by labelling with LTR (red; representing lysosomes) and MTG (green; representing mitochondria). Scale bars: 25 µm. (B ii , D ii ) Quantification showing colocalization (%) defined as Pearson’s co-efficient value calculated from images of each condition of three independent experiments using the JACoP plugin of ImageJ. Data were normalized to the MOCK or shNC cells (mean ± S.D., n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.
    Figure Legend Snippet: Gain and loss of function of BAX regulate CLU-mediated mitophagy during cisplatin treatment. (A, C) Western blot analysis in CLU-OE FaDu and DU 145 cells with loss of function (transfected with shNC, sh BAX ) or gain of function (transfected with vector, BAX ) of BAX followed by cisplatin treatment, respectively. (B i , D i ) Confocal microscopy-based live-cell imaging of cisplatin-treated CLU-OE FaDu and DU 145 cells with loss of function (transfected with shNC, sh BAX ) and gain of function (transfected with vector, BAX ) of BAX followed by labelling with LTR (red; representing lysosomes) and MTG (green; representing mitochondria). Scale bars: 25 µm. (B ii , D ii ) Quantification showing colocalization (%) defined as Pearson’s co-efficient value calculated from images of each condition of three independent experiments using the JACoP plugin of ImageJ. Data were normalized to the MOCK or shNC cells (mean ± S.D., n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.

    Techniques Used: Western Blot, Transfection, Plasmid Preparation, Confocal Microscopy, Live Cell Imaging

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    • du 145 atcc htb 81 rrid cvcl 0105  (ATCC)
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    ATCC du 145 atcc htb 81 rrid cvcl 0105
    Du 145 Atcc Htb 81 Rrid Cvcl 0105, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/du 145 atcc htb 81 rrid cvcl 0105/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    du 145 atcc htb 81 rrid cvcl 0105 - by Bioz Stars, 2024-09
    86/100 stars
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