dttp  (Jena Bioscience)


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  • 93
    Name:
    dTTP Solution
    Description:

    Catalog Number:
    NU-1004-10ML
    Price:
    525.1
    Category:
    Molecular Biology
    Size:
    10 ml
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    Structured Review

    Jena Bioscience dttp

    https://www.bioz.com/result/dttp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dttp - by Bioz Stars, 2021-06
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Low ribosomal RNA genes copy number provoke genomic instability and chromosomal segment duplication events that modify global gene expression and plant-pathogen response
    Article Snippet: The BAC probes (T5H22, F5I10, available at www.arabidopsis.org) were kindly provided by T.Mandáková (CEITEC MU, Brno, CR). .. Approximately 1 µg of isolated BAC DNA (NucleoBond® Xtra Midi kit, Macherey-Nagel) were labeled by nick translation with biotin-dUTP or digoxigenin-dUTP (Jena Bioscience) using either commercial nick translation labelling kit (#07J00-001; Abbot - labelling was performed according to the manufactureŕs instructions using 5µl of the enzyme mix and 8h labelling time); or by home-made reaction mix , containing 5 µL of 10× NT buffer (0.5 M Tris-HCl, pH 7.5, 50 mM MgCl2, and 0.05% BSA), 10mM β-mercaptoethanol, 0.2mM each dATP, dCTP, dGTP and 40 µM dTTP (Jena Bioscience), 0.2 mM labelled-dUTP, 3 µL of DNase I (#10104159001, Roche, diluted to 8 µg/ml) and 5U of DNA polymerase I (#EP0041,Fermentas).The nick translation mixture was incubated at 15°C for ∼ 4h (or longer) to obtain a fragment length of ∼200 to 500 bp. .. The reaction was stopped by adding 1 µL of 0.5 M EDTA, pH 8.0, and incubation at 65°C for 10 min.

    BAC Assay:

    Article Title: Low ribosomal RNA genes copy number provoke genomic instability and chromosomal segment duplication events that modify global gene expression and plant-pathogen response
    Article Snippet: The BAC probes (T5H22, F5I10, available at www.arabidopsis.org) were kindly provided by T.Mandáková (CEITEC MU, Brno, CR). .. Approximately 1 µg of isolated BAC DNA (NucleoBond® Xtra Midi kit, Macherey-Nagel) were labeled by nick translation with biotin-dUTP or digoxigenin-dUTP (Jena Bioscience) using either commercial nick translation labelling kit (#07J00-001; Abbot - labelling was performed according to the manufactureŕs instructions using 5µl of the enzyme mix and 8h labelling time); or by home-made reaction mix , containing 5 µL of 10× NT buffer (0.5 M Tris-HCl, pH 7.5, 50 mM MgCl2, and 0.05% BSA), 10mM β-mercaptoethanol, 0.2mM each dATP, dCTP, dGTP and 40 µM dTTP (Jena Bioscience), 0.2 mM labelled-dUTP, 3 µL of DNase I (#10104159001, Roche, diluted to 8 µg/ml) and 5U of DNA polymerase I (#EP0041,Fermentas).The nick translation mixture was incubated at 15°C for ∼ 4h (or longer) to obtain a fragment length of ∼200 to 500 bp. .. The reaction was stopped by adding 1 µL of 0.5 M EDTA, pH 8.0, and incubation at 65°C for 10 min.

    Labeling:

    Article Title: Low ribosomal RNA genes copy number provoke genomic instability and chromosomal segment duplication events that modify global gene expression and plant-pathogen response
    Article Snippet: The BAC probes (T5H22, F5I10, available at www.arabidopsis.org) were kindly provided by T.Mandáková (CEITEC MU, Brno, CR). .. Approximately 1 µg of isolated BAC DNA (NucleoBond® Xtra Midi kit, Macherey-Nagel) were labeled by nick translation with biotin-dUTP or digoxigenin-dUTP (Jena Bioscience) using either commercial nick translation labelling kit (#07J00-001; Abbot - labelling was performed according to the manufactureŕs instructions using 5µl of the enzyme mix and 8h labelling time); or by home-made reaction mix , containing 5 µL of 10× NT buffer (0.5 M Tris-HCl, pH 7.5, 50 mM MgCl2, and 0.05% BSA), 10mM β-mercaptoethanol, 0.2mM each dATP, dCTP, dGTP and 40 µM dTTP (Jena Bioscience), 0.2 mM labelled-dUTP, 3 µL of DNase I (#10104159001, Roche, diluted to 8 µg/ml) and 5U of DNA polymerase I (#EP0041,Fermentas).The nick translation mixture was incubated at 15°C for ∼ 4h (or longer) to obtain a fragment length of ∼200 to 500 bp. .. The reaction was stopped by adding 1 µL of 0.5 M EDTA, pH 8.0, and incubation at 65°C for 10 min.

    Nick Translation:

    Article Title: Low ribosomal RNA genes copy number provoke genomic instability and chromosomal segment duplication events that modify global gene expression and plant-pathogen response
    Article Snippet: The BAC probes (T5H22, F5I10, available at www.arabidopsis.org) were kindly provided by T.Mandáková (CEITEC MU, Brno, CR). .. Approximately 1 µg of isolated BAC DNA (NucleoBond® Xtra Midi kit, Macherey-Nagel) were labeled by nick translation with biotin-dUTP or digoxigenin-dUTP (Jena Bioscience) using either commercial nick translation labelling kit (#07J00-001; Abbot - labelling was performed according to the manufactureŕs instructions using 5µl of the enzyme mix and 8h labelling time); or by home-made reaction mix , containing 5 µL of 10× NT buffer (0.5 M Tris-HCl, pH 7.5, 50 mM MgCl2, and 0.05% BSA), 10mM β-mercaptoethanol, 0.2mM each dATP, dCTP, dGTP and 40 µM dTTP (Jena Bioscience), 0.2 mM labelled-dUTP, 3 µL of DNase I (#10104159001, Roche, diluted to 8 µg/ml) and 5U of DNA polymerase I (#EP0041,Fermentas).The nick translation mixture was incubated at 15°C for ∼ 4h (or longer) to obtain a fragment length of ∼200 to 500 bp. .. The reaction was stopped by adding 1 µL of 0.5 M EDTA, pH 8.0, and incubation at 65°C for 10 min.

    Incubation:

    Article Title: Low ribosomal RNA genes copy number provoke genomic instability and chromosomal segment duplication events that modify global gene expression and plant-pathogen response
    Article Snippet: The BAC probes (T5H22, F5I10, available at www.arabidopsis.org) were kindly provided by T.Mandáková (CEITEC MU, Brno, CR). .. Approximately 1 µg of isolated BAC DNA (NucleoBond® Xtra Midi kit, Macherey-Nagel) were labeled by nick translation with biotin-dUTP or digoxigenin-dUTP (Jena Bioscience) using either commercial nick translation labelling kit (#07J00-001; Abbot - labelling was performed according to the manufactureŕs instructions using 5µl of the enzyme mix and 8h labelling time); or by home-made reaction mix , containing 5 µL of 10× NT buffer (0.5 M Tris-HCl, pH 7.5, 50 mM MgCl2, and 0.05% BSA), 10mM β-mercaptoethanol, 0.2mM each dATP, dCTP, dGTP and 40 µM dTTP (Jena Bioscience), 0.2 mM labelled-dUTP, 3 µL of DNase I (#10104159001, Roche, diluted to 8 µg/ml) and 5U of DNA polymerase I (#EP0041,Fermentas).The nick translation mixture was incubated at 15°C for ∼ 4h (or longer) to obtain a fragment length of ∼200 to 500 bp. .. The reaction was stopped by adding 1 µL of 0.5 M EDTA, pH 8.0, and incubation at 65°C for 10 min.

    Concentration Assay:

    Article Title: Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis.
    Article Snippet: .. Two mutant dCTP deaminase-dUTPases from Methanocaldococcus jannaschii were crystallised and the crystal structures were solved: E145A in complex with the substrate analogue alpha,beta-imido-dUTP and E145Q in complex with diphosphate. .. Two mutant dCTP deaminase-dUTPases from Methanocaldococcus jannaschii were crystallised and the crystal structures were solved: E145A in complex with the substrate analogue alpha,beta-imido-dUTP and E145Q in complex with diphosphate.

    Article Title: Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR
    Article Snippet: The titration experiments were performed with a PEAQ-ITC instrument (MicroCal, Northampton, MA, USA) with a cell volume of 200 μL and a 40-μL syringe. .. Lyophilized deoxyadenosine triphosphate, deoxycytidine trisphosphate, deoxyguanosine triphosphate, and deoxythymidine triphosphate (Jena Bioscience, Germany) were dissolved in 1× PCR buffer (50 mM KCl, 10 mM Tris-HCl pH 8, and 4 mM MgCl2 ) to a stock concentration of 10 mM for each dNTP and then used at a concentration of 0.1 mM in the syringe. .. Protein concentration of Klenow fragment (product number EP0054, Thermo Scientific) was determined from the UV absorbance at 280 nm with a BioDrop μLITE instrument (BioDrop, Cambridge, UK) with a path length of 0.5 mm, a molar extinction coefficient of 55,450, and a molecular mass of 68 kDa.

    Polymerase Chain Reaction:

    Article Title: Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR
    Article Snippet: The titration experiments were performed with a PEAQ-ITC instrument (MicroCal, Northampton, MA, USA) with a cell volume of 200 μL and a 40-μL syringe. .. Lyophilized deoxyadenosine triphosphate, deoxycytidine trisphosphate, deoxyguanosine triphosphate, and deoxythymidine triphosphate (Jena Bioscience, Germany) were dissolved in 1× PCR buffer (50 mM KCl, 10 mM Tris-HCl pH 8, and 4 mM MgCl2 ) to a stock concentration of 10 mM for each dNTP and then used at a concentration of 0.1 mM in the syringe. .. Protein concentration of Klenow fragment (product number EP0054, Thermo Scientific) was determined from the UV absorbance at 280 nm with a BioDrop μLITE instrument (BioDrop, Cambridge, UK) with a path length of 0.5 mm, a molar extinction coefficient of 55,450, and a molecular mass of 68 kDa.

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