dtt solution  (Thermo Fisher)


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    Thermo Fisher dtt solution
    Dtt Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtt solution/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dtt solution - by Bioz Stars, 2020-03
    99/100 stars

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    Real-time Polymerase Chain Reaction:

    Article Title: Targeted Profiling of RNA Translation
    Article Snippet: .. Buffer WB (see recipe) Buffer LB (see recipe) Solution Q3 (see recipe) Solution QT (see recipe) Primers (see recipe) Nuclease-free water (Ambion) 100% ethanol (Sigma Aldrich) 100% isopropanol (Sigma Aldrich) 100% chloroform (Sigma Aldrich) 1X PBS (Life Technologies) 1 M Tris-HCl solution, pH 7.4 (Ambion) 5 M NaCl solution (Ambion) 1 M MgCl2 solution (Ambion) 3 M NaOAc (Ambion) 1 M DTT solution (Sigma Aldrich) 100 mM DTT solution (Invitrogen) 1 N NaOH solution (Sigma Aldrich) 100% Triton X-100 (Sigma Aldrich) 100 mg/ml cycloheximide (Sigma Aldrich) TRIzol reagent (Ambion) 15 mg/ml GlycoBlue (Ambion) Qubit RNA HS assay kit and assay tubes (Invitrogen) 100 U/ul RNase I (Ambion) 2 U/ul TurboDNase (Ambion) 20 U/ul SUPERase-In (Ambion) 200 U/ul Superscript III (Invitrogen) 5X Superscript III First-Strand buffer (Invitrogen) 40 mM dNTP mixture (10 mM of each dNTP) (New England Biolabs) 2X SYBR-Select Master Mix (Applied Biosystems) 1.5 ml low-retention microcentrifuge tubes Disposable cell scrapers Blue ice Dry ice Liquid nitrogen Standard thermocycler Standard qPCR analyzer Refrigerated microcentrifuge Qubit fluorometer (Invitrogen) Rotary mixer Solution E (see recipe) Solution Q1 (see recipe) Solution Q2 (see recipe) NOTE: We validated this protocol using human mammary epithelial cell lines, such as MDA-MB-468. .. NOTE: In our experiments, we use a Bio-Rad C1000 Thermo Cycler and an Applied Biosystems 7300 Real-Time PCR System.

    Western Blot:

    Article Title: Targeted Profiling of RNA Translation
    Article Snippet: .. Buffer WB (see recipe) Buffer LB (see recipe) Solution Q3 (see recipe) Solution QT (see recipe) Primers (see recipe) Nuclease-free water (Ambion) 100% ethanol (Sigma Aldrich) 100% isopropanol (Sigma Aldrich) 100% chloroform (Sigma Aldrich) 1X PBS (Life Technologies) 1 M Tris-HCl solution, pH 7.4 (Ambion) 5 M NaCl solution (Ambion) 1 M MgCl2 solution (Ambion) 3 M NaOAc (Ambion) 1 M DTT solution (Sigma Aldrich) 100 mM DTT solution (Invitrogen) 1 N NaOH solution (Sigma Aldrich) 100% Triton X-100 (Sigma Aldrich) 100 mg/ml cycloheximide (Sigma Aldrich) TRIzol reagent (Ambion) 15 mg/ml GlycoBlue (Ambion) Qubit RNA HS assay kit and assay tubes (Invitrogen) 100 U/ul RNase I (Ambion) 2 U/ul TurboDNase (Ambion) 20 U/ul SUPERase-In (Ambion) 200 U/ul Superscript III (Invitrogen) 5X Superscript III First-Strand buffer (Invitrogen) 40 mM dNTP mixture (10 mM of each dNTP) (New England Biolabs) 2X SYBR-Select Master Mix (Applied Biosystems) 1.5 ml low-retention microcentrifuge tubes Disposable cell scrapers Blue ice Dry ice Liquid nitrogen Standard thermocycler Standard qPCR analyzer Refrigerated microcentrifuge Qubit fluorometer (Invitrogen) Rotary mixer Solution E (see recipe) Solution Q1 (see recipe) Solution Q2 (see recipe) NOTE: We validated this protocol using human mammary epithelial cell lines, such as MDA-MB-468. .. NOTE: In our experiments, we use a Bio-Rad C1000 Thermo Cycler and an Applied Biosystems 7300 Real-Time PCR System.

    Multiple Displacement Amplification:

    Article Title: Targeted Profiling of RNA Translation
    Article Snippet: .. Buffer WB (see recipe) Buffer LB (see recipe) Solution Q3 (see recipe) Solution QT (see recipe) Primers (see recipe) Nuclease-free water (Ambion) 100% ethanol (Sigma Aldrich) 100% isopropanol (Sigma Aldrich) 100% chloroform (Sigma Aldrich) 1X PBS (Life Technologies) 1 M Tris-HCl solution, pH 7.4 (Ambion) 5 M NaCl solution (Ambion) 1 M MgCl2 solution (Ambion) 3 M NaOAc (Ambion) 1 M DTT solution (Sigma Aldrich) 100 mM DTT solution (Invitrogen) 1 N NaOH solution (Sigma Aldrich) 100% Triton X-100 (Sigma Aldrich) 100 mg/ml cycloheximide (Sigma Aldrich) TRIzol reagent (Ambion) 15 mg/ml GlycoBlue (Ambion) Qubit RNA HS assay kit and assay tubes (Invitrogen) 100 U/ul RNase I (Ambion) 2 U/ul TurboDNase (Ambion) 20 U/ul SUPERase-In (Ambion) 200 U/ul Superscript III (Invitrogen) 5X Superscript III First-Strand buffer (Invitrogen) 40 mM dNTP mixture (10 mM of each dNTP) (New England Biolabs) 2X SYBR-Select Master Mix (Applied Biosystems) 1.5 ml low-retention microcentrifuge tubes Disposable cell scrapers Blue ice Dry ice Liquid nitrogen Standard thermocycler Standard qPCR analyzer Refrigerated microcentrifuge Qubit fluorometer (Invitrogen) Rotary mixer Solution E (see recipe) Solution Q1 (see recipe) Solution Q2 (see recipe) NOTE: We validated this protocol using human mammary epithelial cell lines, such as MDA-MB-468. .. NOTE: In our experiments, we use a Bio-Rad C1000 Thermo Cycler and an Applied Biosystems 7300 Real-Time PCR System.

    RNA HS Assay:

    Article Title: Targeted Profiling of RNA Translation
    Article Snippet: .. Buffer WB (see recipe) Buffer LB (see recipe) Solution Q3 (see recipe) Solution QT (see recipe) Primers (see recipe) Nuclease-free water (Ambion) 100% ethanol (Sigma Aldrich) 100% isopropanol (Sigma Aldrich) 100% chloroform (Sigma Aldrich) 1X PBS (Life Technologies) 1 M Tris-HCl solution, pH 7.4 (Ambion) 5 M NaCl solution (Ambion) 1 M MgCl2 solution (Ambion) 3 M NaOAc (Ambion) 1 M DTT solution (Sigma Aldrich) 100 mM DTT solution (Invitrogen) 1 N NaOH solution (Sigma Aldrich) 100% Triton X-100 (Sigma Aldrich) 100 mg/ml cycloheximide (Sigma Aldrich) TRIzol reagent (Ambion) 15 mg/ml GlycoBlue (Ambion) Qubit RNA HS assay kit and assay tubes (Invitrogen) 100 U/ul RNase I (Ambion) 2 U/ul TurboDNase (Ambion) 20 U/ul SUPERase-In (Ambion) 200 U/ul Superscript III (Invitrogen) 5X Superscript III First-Strand buffer (Invitrogen) 40 mM dNTP mixture (10 mM of each dNTP) (New England Biolabs) 2X SYBR-Select Master Mix (Applied Biosystems) 1.5 ml low-retention microcentrifuge tubes Disposable cell scrapers Blue ice Dry ice Liquid nitrogen Standard thermocycler Standard qPCR analyzer Refrigerated microcentrifuge Qubit fluorometer (Invitrogen) Rotary mixer Solution E (see recipe) Solution Q1 (see recipe) Solution Q2 (see recipe) NOTE: We validated this protocol using human mammary epithelial cell lines, such as MDA-MB-468. .. NOTE: In our experiments, we use a Bio-Rad C1000 Thermo Cycler and an Applied Biosystems 7300 Real-Time PCR System.

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  • 99
    Thermo Fisher dithiothreitol solution
    Dithiothreitol Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dithiothreitol solution/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dithiothreitol solution - by Bioz Stars, 2020-03
    99/100 stars
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