dtt reducing buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dtt reducing buffer
    Gels following CBB and PAS staining (WMS only) of WMS and PS pellicle formations on hydrophobic particles from different subjects. Lanes: saliva pre (1), saliva post (2), water wash 1 (3), water wash 2 (4), SDS 1 (5), SDS 2 (6) and boiled <t>DTT,</t> <t>LDS</t> and water (7). Boxes highlight clear differences in binding ± TGM. The box highlighted by the arrow indicates an effect only seen in 50% of the samples. It shows what appear to be a possible cross-linked proteins, perhaps statherin and histatin bound higher due to crosslinking.
    Dtt Reducing Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtt reducing buffer/product/Thermo Fisher
    Average 81 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dtt reducing buffer - by Bioz Stars, 2020-03
    81/100 stars

    Related Products / Commonly Used Together

    lds
    bis-tris gel

    Images

    1) Product Images from "What interactions drive the salivary mucosal pellicle formation?"

    Article Title: What interactions drive the salivary mucosal pellicle formation?

    Journal: Colloids and Surfaces. B, Biointerfaces

    doi: 10.1016/j.colsurfb.2014.05.020

    Gels following CBB and PAS staining (WMS only) of WMS and PS pellicle formations on hydrophobic particles from different subjects. Lanes: saliva pre (1), saliva post (2), water wash 1 (3), water wash 2 (4), SDS 1 (5), SDS 2 (6) and boiled DTT, LDS and water (7). Boxes highlight clear differences in binding ± TGM. The box highlighted by the arrow indicates an effect only seen in 50% of the samples. It shows what appear to be a possible cross-linked proteins, perhaps statherin and histatin bound higher due to crosslinking.
    Figure Legend Snippet: Gels following CBB and PAS staining (WMS only) of WMS and PS pellicle formations on hydrophobic particles from different subjects. Lanes: saliva pre (1), saliva post (2), water wash 1 (3), water wash 2 (4), SDS 1 (5), SDS 2 (6) and boiled DTT, LDS and water (7). Boxes highlight clear differences in binding ± TGM. The box highlighted by the arrow indicates an effect only seen in 50% of the samples. It shows what appear to be a possible cross-linked proteins, perhaps statherin and histatin bound higher due to crosslinking.

    Techniques Used: Staining, Binding Assay

    Related Articles

    Electrophoresis:

    Article Title: SIgA Binding to Mucosal Surfaces Is Mediated by Mucin-Mucin Interactions
    Article Snippet: Equal protein amounts of samples were added to LDS sample buffer (1:4) (Invitrogen, Paisley, UK) and DTT reducing buffer (1:10) (Invitrogen), with water to make up volumes and heated at 100°C for 3 minutes. .. Electrophoresis was carried out in MES-SDS running buffer (Invitrogen) according to manufacturer’s instructions.

    Staining:

    Article Title: What interactions drive the salivary mucosal pellicle formation?
    Article Snippet: All samples were prepared with 0.5 M DTT reducing buffer (1:10) (Invitrogen) and LDS sample buffer (1:4) (Invitrogen) and boiled for 3 min. 15 μl of sample was then loaded onto a lane of a 4–12% Bis-Tris gel (Invitrogen) and all samples were run according to manufacturer's instructions in MES-SDS running buffer. .. Following this, proteins were visualised using Coomassie brilliant blue R250 stain (Sigma, Dorset, UK), de-staining was completed in 10% acetic acid.

    Incubation:

    Article Title: What interactions drive the salivary mucosal pellicle formation?
    Article Snippet: 2.3 Protein detection SDS-PAGE was performed on all saliva samples, before and after incubation and on all SDS washes. .. All samples were prepared with 0.5 M DTT reducing buffer (1:10) (Invitrogen) and LDS sample buffer (1:4) (Invitrogen) and boiled for 3 min. 15 μl of sample was then loaded onto a lane of a 4–12% Bis-Tris gel (Invitrogen) and all samples were run according to manufacturer's instructions in MES-SDS running buffer.

    SDS Page:

    Article Title: What interactions drive the salivary mucosal pellicle formation?
    Article Snippet: 2.3 Protein detection SDS-PAGE was performed on all saliva samples, before and after incubation and on all SDS washes. .. All samples were prepared with 0.5 M DTT reducing buffer (1:10) (Invitrogen) and LDS sample buffer (1:4) (Invitrogen) and boiled for 3 min. 15 μl of sample was then loaded onto a lane of a 4–12% Bis-Tris gel (Invitrogen) and all samples were run according to manufacturer's instructions in MES-SDS running buffer.

    Article Title: SIgA Binding to Mucosal Surfaces Is Mediated by Mucin-Mucin Interactions
    Article Snippet: Paragraph title: SDS-PAGE ... Equal protein amounts of samples were added to LDS sample buffer (1:4) (Invitrogen, Paisley, UK) and DTT reducing buffer (1:10) (Invitrogen), with water to make up volumes and heated at 100°C for 3 minutes.

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    Thermo Fisher nupage reducing buffer
    Nupage Reducing Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nupage reducing buffer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nupage reducing buffer - by Bioz Stars, 2020-03
    99/100 stars
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