dtt pt7cfe1 chis vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher dtt pt7cfe1 chis vector
    Dtt Pt7cfe1 Chis Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtt pt7cfe1 chis vector/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dtt pt7cfe1 chis vector - by Bioz Stars, 2020-03
    79/100 stars

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    Concentration Assay:

    Article Title: Engineering artificial cells by combining HeLa-based cell-free expression and ultra-thin double emulsion template
    Article Snippet: .. GADD34 Δ1-240 (stock concentration of 2.3 μM) T7 RNA Polymerase (stock concentration of 5 μM) HeLa lysate (Thermo Fisher Scientific) Mix 1 [27.6 mM Mg(OAc)2 , 168 mM K-HEPES (pH 7.5)] Mix 2 [12.5 mM ATP, 8.36 mM GTP, 8.36 mM CTP, 8.36 mM UTP, 200 mM creatine phosphate, 7.8 mM K-HEPES (pH 7.5), 0.6 mg/ml creatine kinase, 0.3 mM amino acid mixture, 5 mM spermidine, 44.4 mM DTT] pT7CFE1-CHis vector (Thermo Fisher Scientific) MscL-GFP-pT7CFE DNA (stock concentration of 500 ng/μl) GFP-pT7CFE DNA (stock concentration of 500 ng/μl) (Thermo Fisher Scientific) Small unilamellar vesicles (SUVs): 18:1 (Δ9-Cis) PC (DOPC) 1,2-dioleoyl-sn-glycero-3-phosphocholine, 18:1 PS (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-L-serine, 18:1 Liss Rhod PE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), cholesterol Rehydration buffer [10 mM Tris-HCl (pH 7.4), 150 mM NaCl 100 nm membrane (Whatman Nuclepore Track-Etched) Silica microspheres 5 μm (Bangs Laboratories) High salt buffer [10 mM Tris-HCl (pH 7.4), 250 mM NaCl] .. Microcentrifuge tubes Glass test tubes Desiccator Plate Reader Heat block Extruder kit (Avanti Polar Lipids)

    Plasmid Preparation:

    Article Title: Engineering artificial cells by combining HeLa-based cell-free expression and ultra-thin double emulsion template
    Article Snippet: .. GADD34 Δ1-240 (stock concentration of 2.3 μM) T7 RNA Polymerase (stock concentration of 5 μM) HeLa lysate (Thermo Fisher Scientific) Mix 1 [27.6 mM Mg(OAc)2 , 168 mM K-HEPES (pH 7.5)] Mix 2 [12.5 mM ATP, 8.36 mM GTP, 8.36 mM CTP, 8.36 mM UTP, 200 mM creatine phosphate, 7.8 mM K-HEPES (pH 7.5), 0.6 mg/ml creatine kinase, 0.3 mM amino acid mixture, 5 mM spermidine, 44.4 mM DTT] pT7CFE1-CHis vector (Thermo Fisher Scientific) MscL-GFP-pT7CFE DNA (stock concentration of 500 ng/μl) GFP-pT7CFE DNA (stock concentration of 500 ng/μl) (Thermo Fisher Scientific) Small unilamellar vesicles (SUVs): 18:1 (Δ9-Cis) PC (DOPC) 1,2-dioleoyl-sn-glycero-3-phosphocholine, 18:1 PS (DOPS) 1,2-dioleoyl-sn-glycero-3-phospho-L-serine, 18:1 Liss Rhod PE 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), cholesterol Rehydration buffer [10 mM Tris-HCl (pH 7.4), 150 mM NaCl 100 nm membrane (Whatman Nuclepore Track-Etched) Silica microspheres 5 μm (Bangs Laboratories) High salt buffer [10 mM Tris-HCl (pH 7.4), 250 mM NaCl] .. Microcentrifuge tubes Glass test tubes Desiccator Plate Reader Heat block Extruder kit (Avanti Polar Lipids)

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  • 79
    Thermo Fisher dtt pt7cfe1 chis vector
    Dtt Pt7cfe1 Chis Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dtt pt7cfe1 chis vector/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dtt pt7cfe1 chis vector - by Bioz Stars, 2020-03
    79/100 stars
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