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dta powder  (Shanghai Yuanye Biochemicals)
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dta mice  (Jackson Laboratory)
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Jackson Laboratory dta mice
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Dta Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa26 dta b6 129s6 cg gt rosa 26sortm1 dtajpmb j  (Jackson Laboratory)
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Jackson Laboratory rosa26 dta b6 129s6 cg gt rosa 26sortm1 dtajpmb j
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Rosa26 Dta B6 129s6 Cg Gt Rosa 26sortm1 Dtajpmb J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gitr agonist dta 1  (Bio X Cell)
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Bio X Cell anti gitr agonist dta 1
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Anti Gitr Agonist Dta 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dta  (Jackson Laboratory)
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Jackson Laboratory dta
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Dta, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa dta 009669 mice  (Jackson Laboratory)
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Jackson Laboratory rosa dta 009669 mice
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Rosa Dta 009669 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa dta flox flox  (Jackson Laboratory)
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Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Rosa Dta Flox Flox, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rosa26 egfp dta mice  (Jackson Laboratory)
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Jackson Laboratory rosa26 egfp dta mice
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
Rosa26 Egfp Dta Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lfn dta  (Trevigen)
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Trevigen lfn dta
Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced <t>DTA</t> expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left <t>:</t> <t>PDGFRα</t> immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).
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Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced DTA expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left : PDGFRα immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).

Journal: Investigative Ophthalmology & Visual Science

Article Title: Oligodendrocyte Precursor Cells Shape Retinogeniculate Refinement Via a CHD8-Dependent Phagocytic Pathway

doi: 10.1167/iovs.67.3.64

Figure Lengend Snippet: Depletion of OPCs impairs synaptic elimination in dLGN. ( A ) Top : Diagram of Olig2 -TK mouse generation. Bottom : Schematic of OLIG2 + mitotic cells ablation. ( B ) Left : OLIG2 immunostaining in the dLGN of P14 wild-type (Ctrl) and Olig2 -TK mice. Scale bar = 100 µm. Right : Quantification of OLIG2 + cells. ( C ) Left : Immunolabeling for Synapsin + pre-synapses around MAP2 + neurons in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( D ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( E ) Left : Immunostaining for vGAT in dLGN of P14 Ctrl and Olig2 -TK mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area. ( F ) Top : Diagram of tamoxifen (TAM) administration induced DTA expression. Bottom : Schematic of TAM treatment from P3 to P7 for OPC depletion. ( G ) Left : PDGFRα immunolabeling in dLGN of P14 PDGFRα-Cre ERT (Ctrl) and PDGFRα-Cre ERT : DTA ( Pα -iDTA) mice. Scale bar = 100 µm. Right : Quantification of PDGFRα + cells. ( H ) Left : Immunolabeling for Synapsin + presynapses around MAP2 + neurons in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of Synapsin + signal around a single neuron. ( I ) Left : Representative images of vGLUT2 and HOMER1 immunostaining in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 25 µm. Right : Quantification of vGLUT2 + /HOMER1 + synapse puncta. ( J ) Left : vGAT immunolabeling in the dLGN of P14 Ctrl and Pα -iDTA mice. Scale bar = 50 µm. Right : Quantification of percent vGAT + area ( n = 5 animals per genotype). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 and n.s., not significant (unpaired, Two-tailed Student's t -test).

Article Snippet: PDGFRα-Cre ERT BAC mice were crossed with DTA mice (JAX, 009669) to generate the Pα -iDTA.

Techniques: Immunostaining, Immunolabeling, Expressing, Two Tailed Test