n desulfated heparin  (AMS Biotechnology)

Journal: Marine Drugs

Article Title: Marine-Derived Sulfated Glycans Inhibit the Interaction of Heparin with Adhesion Proteins of Mycoplasma pneumoniae

doi: 10.3390/md22050232

Figure Lengend Snippet: M. pneumoniae proteins–heparin interaction inhibited by heparin oligosaccharides and desulfated heparins using solution competition. ( A ) SPR sensorgrams of protein P30–heparin interaction competing with different heparin oligosaccharides. ( B ) Bar graphs of protein P30 binding preference to surface heparin by competing with different heparin oligosaccharides. ( C ) SPR sensorgrams of protein P1-C–heparin interaction competing with different heparin oligosaccharides. ( D ) Bar graphs of protein P1-C binding preference to surface heparin by competing with different heparin oligosaccharides. ( E ) SPR sensorgrams of protein P30–heparin interaction competing with different desulfated heparins. ( F ) Bar graphs of protein P30 binding preference to surface heparin by competing with different desulfated heparins. ( G ) SPR sensorgrams of protein P1-C–heparin interaction competing with different desulfated heparins. ( H ) Bar graphs of protein P1-C binding preference to surface heparin by competing with different desulfated heparins. Data are shown as mean ± SD and are analyzed using a one-way ANOVA/Tukey test. Significance is defined as p > 0.05 (ns), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

Article Snippet: 6- O -desulfated heparin(6-DeS heparin, MW of 13 kDa) was provided by Dr. Wang from the University of South Florida.2- O -desulfated IdoA heparin (2-DeS heparin, MW of 13 kDa) and N -desulfated heparin (N-DeS heparin, MW of 14 kDa) were synthesized in our laboratory as per the methods described by Yates et al. Heparin oligosaccharides with varying degrees of polymerization (dp4, dp6, dp8, dp10, dp12, dp14, dp16, and dp18) were acquired from Iduron (Manchester, UK).

Techniques: Binding Assay