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Journal: bioRxiv
Article Title: Clonal relationships between Tph and Tfh cells in patients with SLE and in murine lupus
doi: 10.1101/2025.01.27.635189
Figure Lengend Snippet: A . UMAP plot of CD4 T cells (both spleen and lung) from 3 month treated Bcl6 fl/fl CD4Cre-/+ mice. B, C. Subclustering analysis of cluster 16 from spleen ( B ) or cluster 10 from lung ( C ) showing the DimPlot clusters (LHS) and module enrichment analysis for Tph (red) or Tfh (blue) signatures derived from DEG analysis of 12-month pristane treated mice comparing Tph and Tfh clusters in the spleen. D . DimPlot showing TCR sharing of Tfh subclusters from the spleen (Spleen SC1) or lung (Lung SC5), and Tph-like subclusters in spleen (Spleen SC0 and SC5) or lung (lung SC2). E. Upset plot of TCR sharing incidence between the lung cluster 10 and spleen cluster 16 subclusters. F . Grouped bar graph showing cluster count stratified by genotype for Il21 + clusters 10 and 16. G, H . Grouped bar graph showing sub-cluster cell counts in either the spleen ( G ) or lung ( H ) stratified by genotype. I . ELISA of total IgG and dsDNA-IgG in wildtype or Bcl6 fl/fl CD4Cre + mice. Mann-Whitney U test, ** p = < 0.01, n=4-6 per group.
Article Snippet: Total IgG and
Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY