Structured Review

Horizon Discovery doxycycline inducible ptripz derived vector
Doxycycline Inducible Ptripz Derived Vector, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/doxycycline inducible ptripz derived vector/product/Horizon Discovery
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
doxycycline inducible ptripz derived vector - by Bioz Stars, 2020-04
92/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP).

Amplification:

Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP).

Plasmid Preparation:

Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
Article Snippet: .. A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP).

Expressing:

Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
Article Snippet: .. A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP).