doxycycline inducible ptrip vectors  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Thermo Fisher doxycycline inducible ptrip vectors
    Activation of SMAD signaling at low cell density and contribution of ROS signaling. ( A ) <t>Smad7</t> overexpression changes the gene expression profile of LNCaP* cells under LD_hypo conditions. Ratios of mRNA species levels measured by RT-qPCR in <t>pTRIP-mSMAD7</t> and pTRIP-control transduced cell populations cultured under LD_hypo conditions were averaged from 2 experiments using independent cell populations. Stars indicate significance of the decreased mRNA species accumulation upon murine Smad7 overexpression. ( B ) Glutathione supplementation reduces the activation of SMAD signaling at low density. Relative mRNA levels measured by RT-qPCR under LD_hypo, LD_hypo+glutathione and MD_hypo conditions were averaged from 4–5, 2 and 7–9 cell culture experiments, respectively. Stars indicate statistical significance of the difference in mRNA levels between cells cultured in LD_hypo and LD_hypo+glutathione. ( C ) Lack of additive effects between Smad7 overexpression and glutathione treatment on cloning efficiency. Cloning efficiency of pTRIP-control and pTRIP-m Smad 7 transduced cell populations were measured under the indicated cell culture conditions. Data were averaged from 2 to 5 experiments made with 2 independent control and 2 independent Smad7 transduced cell populations processed in parallel. Stars indicate significance of the differences in cloning efficiency. Data are presented as mean ± s.d.
    Doxycycline Inducible Ptrip Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible ptrip vectors/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible ptrip vectors - by Bioz Stars, 2020-05
    88/100 stars

    Images

    1) Product Images from "SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy"

    Article Title: SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy

    Journal: Cell Cycle

    doi: 10.1080/15384101.2015.1014145

    Activation of SMAD signaling at low cell density and contribution of ROS signaling. ( A ) Smad7 overexpression changes the gene expression profile of LNCaP* cells under LD_hypo conditions. Ratios of mRNA species levels measured by RT-qPCR in pTRIP-mSMAD7 and pTRIP-control transduced cell populations cultured under LD_hypo conditions were averaged from 2 experiments using independent cell populations. Stars indicate significance of the decreased mRNA species accumulation upon murine Smad7 overexpression. ( B ) Glutathione supplementation reduces the activation of SMAD signaling at low density. Relative mRNA levels measured by RT-qPCR under LD_hypo, LD_hypo+glutathione and MD_hypo conditions were averaged from 4–5, 2 and 7–9 cell culture experiments, respectively. Stars indicate statistical significance of the difference in mRNA levels between cells cultured in LD_hypo and LD_hypo+glutathione. ( C ) Lack of additive effects between Smad7 overexpression and glutathione treatment on cloning efficiency. Cloning efficiency of pTRIP-control and pTRIP-m Smad 7 transduced cell populations were measured under the indicated cell culture conditions. Data were averaged from 2 to 5 experiments made with 2 independent control and 2 independent Smad7 transduced cell populations processed in parallel. Stars indicate significance of the differences in cloning efficiency. Data are presented as mean ± s.d.
    Figure Legend Snippet: Activation of SMAD signaling at low cell density and contribution of ROS signaling. ( A ) Smad7 overexpression changes the gene expression profile of LNCaP* cells under LD_hypo conditions. Ratios of mRNA species levels measured by RT-qPCR in pTRIP-mSMAD7 and pTRIP-control transduced cell populations cultured under LD_hypo conditions were averaged from 2 experiments using independent cell populations. Stars indicate significance of the decreased mRNA species accumulation upon murine Smad7 overexpression. ( B ) Glutathione supplementation reduces the activation of SMAD signaling at low density. Relative mRNA levels measured by RT-qPCR under LD_hypo, LD_hypo+glutathione and MD_hypo conditions were averaged from 4–5, 2 and 7–9 cell culture experiments, respectively. Stars indicate statistical significance of the difference in mRNA levels between cells cultured in LD_hypo and LD_hypo+glutathione. ( C ) Lack of additive effects between Smad7 overexpression and glutathione treatment on cloning efficiency. Cloning efficiency of pTRIP-control and pTRIP-m Smad 7 transduced cell populations were measured under the indicated cell culture conditions. Data were averaged from 2 to 5 experiments made with 2 independent control and 2 independent Smad7 transduced cell populations processed in parallel. Stars indicate significance of the differences in cloning efficiency. Data are presented as mean ± s.d.

    Techniques Used: Activation Assay, Over Expression, Expressing, Quantitative RT-PCR, Cell Culture, Clone Assay

    Related Articles

    Expressing:

    Article Title: SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy
    Article Snippet: .. Doxycycline-inducible pTRIP vectors for murine Smad7 (m Smad7 ) and human BMP4 cDNA expression were constructed by replacing the RFP-shRNA cassette downstream the TET/ON promoter in pTRIPZ- shp21 (Open Biosystems, Thermofisher Scientific Biosciences) with the corresponding cDNA. .. Excision of the RFP-shRNA cassette was achieved by Age 1- Mlu 1 digestion of pTRIPZ- shp21 followed by Klenow treatment. mSmad7 cDNA was recovered from pBabepuro-m Smad7 (previously constructed by T.T. from murine F9 cell cDNA) by BamH1-SphI digestion and Klenow blunting, BMP4 cDNA was derived from pCMV-Sport6-hBMP4 (sequence verified cDNA clone IMAGE n° 4399276 from RZPD; German Science Center for Genome Research) by Xho 1- Age 1 digestion and Klenow blunting. pTRIP-control vector refers to pTRIPZ-shp21 following excision of the RFP-shRNA cassette. pSuperRetro- sh-SMAD4 was constructed as previously described.

    Construct:

    Article Title: SMAD signaling and redox imbalance cooperate to induce prostate cancer cell dormancy
    Article Snippet: .. Doxycycline-inducible pTRIP vectors for murine Smad7 (m Smad7 ) and human BMP4 cDNA expression were constructed by replacing the RFP-shRNA cassette downstream the TET/ON promoter in pTRIPZ- shp21 (Open Biosystems, Thermofisher Scientific Biosciences) with the corresponding cDNA. .. Excision of the RFP-shRNA cassette was achieved by Age 1- Mlu 1 digestion of pTRIPZ- shp21 followed by Klenow treatment. mSmad7 cDNA was recovered from pBabepuro-m Smad7 (previously constructed by T.T. from murine F9 cell cDNA) by BamH1-SphI digestion and Klenow blunting, BMP4 cDNA was derived from pCMV-Sport6-hBMP4 (sequence verified cDNA clone IMAGE n° 4399276 from RZPD; German Science Center for Genome Research) by Xho 1- Age 1 digestion and Klenow blunting. pTRIP-control vector refers to pTRIPZ-shp21 following excision of the RFP-shRNA cassette. pSuperRetro- sh-SMAD4 was constructed as previously described.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Thermo Fisher doxycycline inducible ptripz vector
    Doxycycline Inducible Ptripz Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible ptripz vector/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible ptripz vector - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results