doxycycline inducible lentiviral vector ptripz  (Thermo Fisher)


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    Structured Review

    Thermo Fisher doxycycline inducible lentiviral vector ptripz
    Doxycycline Inducible Lentiviral Vector Ptripz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible lentiviral vector ptripz/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible lentiviral vector ptripz - by Bioz Stars, 2020-03
    88/100 stars

    Related Products / Commonly Used Together

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    rat mef2c

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    Related Articles

    Clone Assay:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: .. The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment. .. Lentivirus was generated in HEK293T cells through cotransfection of the psPAX2 and pMD2.G packaging plasmids and used to transinfect SCC cell lines.

    Transfection:

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. 3μM doxycycline was added for 72 h after transfection to induce shRNA expression.

    shRNA:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment. .. GIPZ Human GRHL3 shRNA (Clone ID: V2LHS_65975, Thermoscientific, MA) was used to knock down GRHL3 in OKF-6 and HaCaT cells, as described previously ( ).

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. 3μM doxycycline was added for 72 h after transfection to induce shRNA expression.

    Expressing:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: Paragraph title: Cell Culture, Expression of S9A GSK3B, and Knockdown of GRHL3 ... The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment.

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. 3μM doxycycline was added for 72 h after transfection to induce shRNA expression.

    Luciferase:

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: .. An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. 3μM doxycycline was added for 72 h after transfection to induce shRNA expression.

    Infection:

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. Cultured rat cortical neurons were infected on DIV1.

    Generated:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment. .. Lentivirus was generated in HEK293T cells through cotransfection of the psPAX2 and pMD2.G packaging plasmids and used to transinfect SCC cell lines.

    Cell Culture:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: Paragraph title: Cell Culture, Expression of S9A GSK3B, and Knockdown of GRHL3 ... The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment.

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. Cultured rat cortical neurons were infected on DIV1.

    Construct:

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. MEF2 knockdown constructs targeting rat MEF2A, C or D in the vector pLKO.1 (TRCN0000095959, TRCN0000012068, and TRCN0000085268) were obtained (Thermo Scientific) and transfected for luciferase assays or packaged as lentiviral particles in 293T cells.

    Cotransfection:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment. .. Lentivirus was generated in HEK293T cells through cotransfection of the psPAX2 and pMD2.G packaging plasmids and used to transinfect SCC cell lines.

    Modification:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: HaCaT, HEK293T, SCC-1, SCC-47, SCC-22B, SQ-20b, Detroit 562, and FaDu cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum. .. The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment.

    Quantitative RT-PCR:

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: Paragraph title: RNA interference (RNAi) plasmids and quantitative RT-PCR ... An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays.

    Plasmid Preparation:

    Article Title: Identification of a Novel Proto-oncogenic Network in Head and Neck Squamous Cell Carcinoma
    Article Snippet: .. The S9A GSK3B cDNA was obtained in the Addgene plasmid 14754 ( ) and cloned into the doxycycline-inducible lentiviral vector pTRIPZ (Thermoscientific, MA) as an Xho I/ Mlu I fragment. .. Lentivirus was generated in HEK293T cells through cotransfection of the psPAX2 and pMD2.G packaging plasmids and used to transinfect SCC cell lines.

    Article Title: Members of the MEF2 transcription factor family differentially regulate Bdnf transcription in response to neuronal depolarization
    Article Snippet: .. An shRNAmir plasmid targeting rat MEF2C in the doxycycline-inducible lentiviral vector pTRIPZ (V2THS_151686, Thermo Scientific, Lafayette, CO) was used in luciferase assays. .. 3μM doxycycline was added for 72 h after transfection to induce shRNA expression.

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  • 88
    Thermo Fisher doxycycline inducible lentiviral vector ptripz
    Doxycycline Inducible Lentiviral Vector Ptripz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible lentiviral vector ptripz/product/Thermo Fisher
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible lentiviral vector ptripz - by Bioz Stars, 2020-03
    88/100 stars
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