doxycycline inducible lentiviral vector ptripz ctrl  (Thermo Fisher)


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    Structured Review

    Thermo Fisher doxycycline inducible lentiviral vector ptripz ctrl
    Doxycycline Inducible Lentiviral Vector Ptripz Ctrl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible lentiviral vector ptripz ctrl/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible lentiviral vector ptripz ctrl - by Bioz Stars, 2020-07
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Amplification:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Synthesized:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Mutagenesis:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Construct:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Polymerase Chain Reaction:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

    Plasmid Preparation:

    Article Title: Direct non-productive HIV-1 infection in a T-cell line is driven by cellular activation state and NF?B
    Article Snippet: .. The Red-Blue-HIV-1 (RBH) molecular clone was created by cloning a synthesized tagBFP construct (GeneWiz) into the SapI/SphI sites of RGH. pTRIPz-EV, pTRIPz-DN-IκBα and pTRIPz-p65 are derivatives of the commercial doxycycline-inducible lentiviral vector pTRIPZ-Ctrl (Thermo Fisher). pTRIPz-EV (empty vector) was created by digestion with AgeI/MluI , blunting with Klenow polymerase, and re-ligation. pTRIPz-DN-IκBα contains the S32A/S36A mutant version of the IκBα repressor PCR amplified from pSVK3-IKBα-2N [ ], which was cloned into the AgeI/MluI sites of pTRIPz-Ctrl. pTRIPz-p65 contains a PCR amplified NFκB p65 open reading frame cloned into the AgeI/MluI sites of pTRIPz-Ctrl. .. Cell culture, virion production, and transduction Jurkat E6-1 [ ], HEK293T (ATCC), and derivative cell lines created in this study were cultured as previously described [ ].

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  • 86
    Thermo Fisher doxycycline inducible lentiviral vector ptripz ctrl
    Doxycycline Inducible Lentiviral Vector Ptripz Ctrl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/doxycycline inducible lentiviral vector ptripz ctrl/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    doxycycline inducible lentiviral vector ptripz ctrl - by Bioz Stars, 2020-07
    86/100 stars
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